Cell Meter VX450固定化细胞活性染料 货号22540-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Cell Meter VX450固定化细胞活性染料

Cell Meter VX450固定化细胞活性染料

Cell Meter VX450固定化细胞活性染料    货号22540 货号 22540 存储条件 在零下15度以下保存, 避免光照
规格 200 Tests 价格 2412
Ex (nm) 406 Em (nm) 445
分子量 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:22540

产品名称:Cell Meter VX450固定化细胞活性染料

规格:200 Tests

储存条件:保存在冰箱-15℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

激发波长(nm):406

发射波长(nm):445

 

适用仪器


流式细胞仪  
激发: 405nm激光
发射: 525/40nm滤波片
通道: AmCyan通道
荧光显微镜  
激发: 紫色滤波片组
发射: 紫色滤波片组
推荐孔板: 黑色透明

 

产品介绍

从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群。Cell Meter VX450固定化细胞活性染料是一类细胞不可渗透的荧光活性染料,它们经过优化以匹配常见流式细胞仪的主要激发激光,例如350、405、488、633和647 nm。这些染料不渗入活细胞,但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter VX450固定化细胞活性染料发生反应,因此比具有完整膜的活细胞的染色更亮,因此这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时,需要考虑一些因素,例如,每种染料的滴定,以确保活细胞几乎没有染色。Cell Meter VX450固定化细胞活性染料经过优化,可以在405 nm的紫色激光激发下以450 nm的波长发射。与其他商业上类似的活性染料相比,这种固定化细胞活性染料更加耐用、稳定。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Cell Meter VX450固定化细胞活性染料。 

点击查看光谱

 

图示

 

 

Cell Meter VX450固定化细胞活性染料    货号22540

图1.通过Cell Meter 固定化活性染料检测Jurkat细胞活性。处理Jurkat细胞并用Cell Meter VX450(Cat#22540)染色,然后固定在3.7%甲醛中并通过流式细胞仪进行分析。通过AmCyan通道可以很容易地将死细胞群体(蓝色峰)与活细胞群体(红色峰)区分开,在固定之前和之后都获得了几乎相同的结果。

 

 

参考文献

CFSE dilution to study human T and NK cell proliferation in vitro.
Authors: Terrén, Iñigo and Orrantia, Ane and Vitallé, Joana and Zenarruzabeitia, Olatz and Borrego, Francisco
Journal: Methods in enzymology (2020): 239-255

PGC-1α Regulates Cell Proliferation and Invasion via AKT/GSK-3β/β-catenin Pathway in Human Colorectal Cancer SW620 and SW480 Cells.
Authors: Yun, Seong-Hoon and Park, Joo-In
Journal: Anticancer research (2020): 653-664

The miR-3940-5p inhibits cell proliferation of gingival mesenchymal stem cells.
Authors: Han, Xiao and Yang, Haoqing and Cao, Yangyang and Ge, Lihua and Han, Nannan and Zhang, Chen and Fan, Zhipeng and Yao, Rui
Journal: Oral diseases (2019): 1363-1373

Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing.
Authors: Nagarkatti, Mitzi and Rieder, Sadiye Amcaoglu and Nagarkatti, Prakash S
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 209-230

Toll-Like Receptor 4 (TLR4)/Cyclooxygenase-2 (COX-2) Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion by NF-κB Activation.
Authors: Wang, Wei and Wang, Jiye
Journal: Medical science monitor : international medical journal of experimental and clinical research (2018): 5588-5597

Gag-Specific CD4 T Cell Proliferation, Plasmacytoid Dendritic Cells, and Ethnicity in Perinatally HIV-1-Infected Youths: The ANRS-EP38-IMMIP Study.
Authors: Scott-Algara, Daniel and Warszawski, Josiane and Chenadec, Jérôme Le and Didier, Céline and Montange, Thomas and Viard, Jean-Paul and Dollfus, Catherine and Avettand-Fenoel, Véronique and Rouzioux, Christine and Blanche, Stéphane and Buseyne, Florence
Journal: AIDS research and human retroviruses (2017): 21-28

Quantitative analysis of cell proliferation by a dye dilution assay: Application to cell lines and cocultures.
Authors: Chung, Soobin and Kim, Seol-Hee and Seo, Yuri and Kim, Sook-Kyung and Lee, Ji Youn
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2017): 704-712

[Myeloid-derived suppressor cells promoted autologous B cell proliferation in rheumatoid arthritis].
Authors: Chen, W and Hu, F L and Liu, H J and Xu, L L and Li, Y N and Li, Z G
Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (2017): 819-823

A comparative study of colorimetric cell proliferation assays in immune cells.
Authors: Koyanagi, Madoka and Kawakabe, So and Arimura, Yutaka
Journal: Cytotechnology (2016): 1489-98

Hypoxia inhibits mesenchymal stem cell proliferation through HIF1α-dependent regulation of P27.
Authors: Kumar, Sanjay and Vaidya, Meenal
Journal: Molecular and cellular biochemistry (2016): 29-38

说明书
Cell Meter VX450固定化细胞活性染料.pdf