钙红素 AM

钙红素 AM

	货号	22011	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	2544
	Ex (nm)	643	Em (nm)	663
	分子量	~1000	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：22011

产品名称：钙红素 AM

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：~1000

溶剂：DMSO

激发波长(nm)：643

发射波长(nm)：663

 

 

产品介绍

钙黄绿素AM是最流行的荧光探针之一，用于标记和监测活细胞的细胞功能。但是，钙黄绿素AM的单色使得无法在多色应用中使用这种有价值的试剂。例如，与GFP相同的颜色，不可能将Calcein AM与GFP转染的细胞结合使用。为了解决钙黄绿素AM的颜色限制，我们开发了Calcein Orange，Calcein Red和Calcein Deep Red。这些新的钙黄绿素AM酯与钙黄绿素AM结合使用可对活细胞进行多色标记和功能分析。非荧光钙红绿素 AM酯很容易进入活细胞并水解，产生强荧光的钙红绿素 （Cat＃：21902）染料。钙红绿素染料可以用通用的Cy5滤波器组进行检测。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的钙红素 AM。 

点击查看光谱

 

图示

图1.用钙黄绿素Deep Red AM酯（Cat＃22011）染色的Jurkat细胞的流式细胞术分析。将Jurkat细胞用HH缓冲液洗涤一次，并在37°C下用含0.02％PF-127（Cat＃20053）和1mM PBC（Cat＃20061）的2 uM Calcein Deep Red AM酯（Cat＃22011）染色30分钟。然后用HH缓冲液洗涤细胞，并重悬于HH缓冲液中。使用蓝色激光APC发射通道，用NovoCyte 3000流式细胞仪测量活细胞（红色）和死细胞（在55°C水浴中处理30分钟，绿色）的荧光强度。 

 

参考文献

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Factors Affecting the Acoustic In Vitro Release of Calcein from PEGylated Liposomes
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Ratio fluorometric determination of ATP base on the reversion of fluorescence of calcein quenched by Eu(III) ion using carbon dots as reference
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Calcein Release from Cells In Vitro via Reversible and Irreversible Electroporation
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Calcein leakage as a robust assay for cytochrome c/H2O2-mediated liposome permeabilization
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Light scattering corrections to linear dichroism spectroscopy for liposomes in shear flow using calcein fluorescence and modified Rayleigh-Gans-Debye-Mie scattering
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A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis
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