ddCTP [2′,3′-Dideoxycytidine-5′-triphosphate] 货号17207-AAT Bioquest荧光染料

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ddCTP [2′,3′-Dideoxycytidine-5′-triphosphate]

ddCTP [2′,3′-Dideoxycytidine-5′-triphosphate]

ddCTP [2',3'-Dideoxycytidine-5'-triphosphate]    货号17207 货号 17207 存储条件 在零下15度以下保存, 避免光照
规格 1 umole 价格 2412
Ex (nm) Em (nm)
分子量 451.16 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17207

产品名称:ddCTP [2′,3′-Dideoxycytidine-5′-triphosphate]

规格:1 umole

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:451.16

溶剂:水

 

产品介绍

Sanger测序,也称为链终止法,是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸(ddNTPs)的DNA测序技术。它是由Frederick Sanger及其同事于1977年开发的。尽管新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍,但Sanger测序仍具有99.99%的准确度,仍然是临床的“黄金标准”研究排序。 dd-CTP是执行Sanger测序的四个关键ddNTP组件之一。

 

参考文献

Involvement of DNA polymerase beta in DNA replication and mutagenic consequences.
Authors: Servant, Laurence and Bieth, Anne and Hayakawa, Hiroshi and Cazaux, Christophe and Hoffmann, Jean-Sébastien
Journal: Journal of molecular biology (2002): 1039-47

Fluorescence-based DHPLC for allelic quantification by single-nucleotide primer extension.
Authors: Kosaki, K and Yoshihashi, H and Ohashi, Y and Kosaki, R and Suzuki, T and Matsuo, N
Journal: Journal of biochemical and biophysical methods (2001): 111-9

Denaturation fingerprinting: two related mutation detection methods especially advantageous for high G + C regions.
Authors: Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S
Journal: BioTechniques (1998): 140-7

Synergistic inhibition of HIV-1 reverse transcriptase by combinations of chain-terminating nucleotides.
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31

A fidelity assay using “dideoxy” DNA sequencing: a measurement of sequence dependence and frequency of forming 5-bromouracil X guanine base mispairs.
Authors: Lasken, R S and Goodman, M F
Journal: Proceedings of the National Academy of Sciences of the United States of America (1985): 1301-5

说明书
ddCTP [2′,3′-Dideoxycytidine-5′-triphosphate].pdf