DAPI（4′,6-二脒基-2-苯基吲哚二盐酸盐）*10mM水溶液* CAS 28718-90-3

	货号	17507	存储条件	在零下15度以下保存, 避免光照
	规格	2 mL	价格	984
	Ex (nm)	359	Em (nm)	457
	分子量	350.25	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17507

产品名称：DAPI[4,6-二脒基-2-苯基吲哚，二盐酸盐]* 10 mM水溶液*

CAS：28718-90-3

规格：2ml

储存条件：保存在冰箱-20℃干燥

保质期：6个月

产品物理化学光谱特性

分子量：350.25

溶剂：水

Ex(nm):358

Em(nm):461

产品介绍

DAPI是一种与DNA结合强烈的荧光染色剂。它广泛用于荧光显微镜。由于DAPI通过完整的细胞膜，它可用于除固定细胞外的活细胞染色。对于荧光显微镜，DAPI被紫外线激发。当与双链DNA结合时，其最大吸收在358nm，其发射最大值在461nm。DAPI的一个缺点是其排放相当广泛。DAPI也与RNA结合，但是它不像它与DNA结合时那样强烈地发荧光。 当与RNA结合时，其发射偏移至约500nm .DAPI的蓝色发射便于多重测定，因为DAPI与荧光素和绿色荧光蛋白（GFP）等绿色荧光分子或红色荧光染料之间的荧光重叠非常少。除了标记细胞核外，DAPI还用于检测细胞培养物中的支原体或病毒DNA。

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参考文献

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