Screen Quest 多巴胺受体1表达细胞株

	货号	38004	存储条件	在零下80度以下保存
	规格	Each	价格	0
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

产品基本信息

货号：38004

产品名称：Screen Quest 多巴胺受体1表达细胞株

规格：Each

产品介绍

G蛋白偶联受体（GPCR）是药物发现计划针对的最大受体类别之一。钙通量（通过Gq途径耦合）测定法是药物发现中筛选GPCR靶标的首选方法。但是，超过60％的已知GPCR通过与cAMP偶联的腺苷酸环化酶活性发出信号。大多数现有的cAMP分析不仅需要细胞裂解，而且缺乏时间和空间分辨率。 Screen Quest 多巴胺受体1表达细胞株能够研究通常不通过细胞内钙偶联的GPCR。 Screen Quest 多巴胺受体1表达细胞株基于一系列G蛋白嵌合体，包括混杂G蛋白Gα16和外源性环状核苷酸门控通道（CNGC）。嵌合体由Gq蛋白复合物的α亚基组成，该复合物的5个羧基末端氨基酸已被其他G蛋白之一（Gαs，Gαi，Gαo或Gαz）取代。这些氨基酸负责受体与其G蛋白的偶联。这些嵌合体或CNGC与特定的非Gq偶联受体共表达可能会在受体刺激后导致细胞内钙信号的产生。 Screen Quest HEK-CNGC-多巴胺受体1（DRD1）细胞株是用CNGC和人多巴胺受体D1稳定转染的HEK-293细胞。细胞中组成型表达的CNGC通过协调改变阳离子通量（例如钙，钾或钠）实时响应细胞内cAMP水平的升高或降低。可以使用钙敏感的荧光指示剂（例如Calbryte 520 AM，Cal-520 AM，Fluo-8 AM或Fluo-4 AM和相应的免洗钙试剂盒）检测特定配体对DRD1的激活。 ）或AAT优化的膜电位测定试剂盒。该细胞系已成功用于药物发现和筛选环境中，以研究通常不通过细胞内钙偶联的GPCR。它已被FLIPR和FDSS系统有效使用。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Screen Quest 多巴胺受体1表达细胞株。 

参考文献

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