PhosphoWorks 发光法ATP检测试剂盒* DTT – Free* 货号21613-AAT Bioquest荧光染料

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PhosphoWorks 发光法ATP检测试剂盒* DTT – Free*

PhosphoWorks 发光法ATP检测试剂盒* DTT – Free*

PhosphoWorks 发光法ATP检测试剂盒* DTT - Free*    货号21613 货号 21613 存储条件 在零下15度以下保存, 避免光照
规格 10 Plates 价格 12336
Ex (nm) Em (nm)
分子量 溶剂
产品详细介绍

简要概述

PhosphoWorks 发光法ATP检测试剂盒* DTT – Free*是美国AAT Bioquest生产的用于检测ATP的试剂盒,三磷酸腺苷(ATP)在细胞能量生成,代谢调节和细胞信号传导中起着基本作用。 PhosphoWorks ATP检测试剂盒提供了一种快速,简单且均一的发光检测方法,用于测定哺乳动物细胞中的细胞增殖和细胞毒性。 该测定可以以方便的96孔和384孔微量滴定板形式进行。 此测定法的高灵敏度允许在许多生物系统,环境样品和食品中检测ATP。 此PhosphoWorks ATP检测试剂盒不使用DTT,并且具有长达4小时的稳定发光信号。 它具有稳定的发光,无需混合或分离,并且配方具有最小的动手时间。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的PhosphoWorks 发光法ATP检测试剂盒* DTT – Free*。 

 

适用仪器


发光酶标仪  
推荐孔板: 白色孔板

产品说明书

操作步骤

简要概述

1.用测试化合物(100μL/ 96孔板或25μL/ 384孔板)制备细胞(样品)
2.加入等体积的ATP工作溶液(100μL/ 96孔板或25μL/ 384孔板)
3.在室温下孵育10-20分钟
4.监测发光强度

 

制备工作溶液

1.将10 mL反应缓冲液(组分C)转移到ATP传感器(组分B)中并充分混合。

2.将20μLATP监测酶(组分A)加入到组分B + C的瓶中并充分混合以制备ATP工作溶液。 注意:避免来自外源生物来源的潜在ATP污染。

有关细胞样品制备的指南(点击查看)

 

样品实验方案

1.运行ATP测定:

1.1用测试化合物处理细胞(或样品),在96孔板中加入10μL10X化合物,或在所需化合物缓冲液中加入5μL5X化合物用于384孔板。对于空白孔(没有细胞的培养基),加入相应量的化合物缓冲液。

1.2将细胞板在37℃,5%CO 2培养箱中孵育所需的一段时间,例如24,48或96小时。

1.3向每个孔中加入100μL(96孔板)或25μL(384孔板)的ATP工作溶液。

1.4在室温下孵育10-20分钟。

1.5用标准发光计监测发光强度。

 

2.生成标准ATP校准曲线:

        如果需要计算样品中ATP的绝对量,则应与上述分析一起生成ATP标准曲线。

2.1通过包含不含ATP的样品(作为对照)在含有0.1%BSA的PBS缓冲液中制备ATP系列稀释液以测量背景发光。注意:通常ATP浓度范围为0.1 nM至1μM是合适的。

2.2将相同量的稀释的ATP溶液加入空板中(对于96孔板为100μL或对于384孔板为25μL)。

2.3加入100μL/孔(96孔板)或25μL/孔(384孔板)的ATP工作溶液。

2.4将反应混合物在室温下孵育10至20分钟。

2.5用标准发光计记录发光强度。

2.6生成ATP标准曲线。

 

参考文献

High throughput cell-based assay for identification of glycolate oxidase inhibitors as a potential treatment for Primary Hyperoxaluria Type 1
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NT1014, a novel biguanide, inhibits ovarian cancer growth in vitro and in vivo
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Journal: Journal of Hematology & Oncology (2016): 91

The Different Effects of Atorvastatin and Pravastatin on Cell Death and PARP Activity in Pancreatic NIT-1 Cells
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BPA-induced DNA hypermethylation of the master mitochondrial gene PGC-1α contributes to cardiomyopathy in male rats
Authors: Ying Jiang, Wei Xia, Jie Yang, Yingshuang Zhu, Huailong Chang, Juan Liu, Wenqian Huo, Bing Xu, Xi Chen, Yuanyuan Li
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Glutamine promotes ovarian cancer cell proliferation through the mTOR/S6 pathway
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JQ1 suppresses tumor growth through downregulating LDHA in ovarian cancer
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Loss of histone deacetylase Hdac1 disrupts metabolic processes in intestinal epithelial cells
Authors: Alexis Gonneaud, Naomie Turgeon, Frančois-Michel Boisvert, Frančois Boudreau, Claude Asselin
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A neutrophil intrinsic impairment affecting Rab27a and degranulation in cystic fibrosis is corrected by CFTR potentiator therapy
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Fluorescence lifetime imaging unravels C. trachomatis metabolism and its crosstalk with the host cell
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G protein coupled receptor kinase 2 interacting protein 1 (GIT1) is a novel regulator of mitochondrial biogenesis in heart
Authors: Jinjiang Pang, Xiangbin Xu, Michael R Getman, Xi Shi, Stephen L Belmonte, Heidi Michaloski, Amy Mohan, Burns C Blaxall, Bradford C Berk
Journal: Journal of molecular and cellular cardiology (2011): 769–776

 

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说明书
PhosphoWorks 发光法ATP检测试剂盒* DTT – Free*.pdf