钙离子荧光探针Mag-Fura-2, AM CAS 132319-57-4 货号20383-AAT Bioquest荧光染料

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钙离子荧光探针Mag-Fura-2, AM CAS 132319-57-4

钙离子荧光探针Mag-Fura-2, AM CAS 132319-57-4

钙离子荧光探针Mag-Fura-2, AM  CAS 132319-57-4    货号20383 货号 20383 存储条件 在零下15度以下保存, 避免光照
规格 10×50 ug 价格 2544
Ex (nm) 336 Em (nm) 505
分子量 722.56 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:20383

产品名称:钙离子荧光探针Mag-Fura-2, AM 

CAS:132319-57-4

规格:10x50ug

储存条件:-15℃避光防潮

保质期:12个月

化学和光谱特性
分子量:722.56
溶剂:DMSO
激发波长:336
发射波长:503

 

适用仪器


荧光显微镜  
激发: Fura 2滤波片
发射: Fura 2滤波片
通道: 黑色透明
荧光酶标仪  
激发: 340,380nm
发射: 510nm
cutoff: 475nm
推荐孔板: 黑色透明
读取模式 底读模式

 

产品介绍

钙离子荧光探针Mag-Fura-2, AM 是美国AAT Bioquest生产的钙离子荧光探针,Mag-Fura-2,AM是一种细胞内镁指示剂,它是成比例的且可被紫外线激发。它的光谱特性与Fura-2非常匹配。这种乙酰氧基甲基(AM)酯形式可用于无创性细胞内装载。它也用于测量活细胞中的高水平钙离子。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Mag-Fura-2, AM 。 

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点击查看实验方案

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-Fura-2 and Analogues
Authors: Zhang G, Jacquemin D, Buccella D.
Journal: J Phys Chem B. (2017)

Fluorescence measurements of free [Mg2+] by use of mag-fura 2 in Salmonella enterica
Authors: Froschauer EM, Kolisek M, Dieterich F, Schweigel M, Schweyen RJ.
Journal: FEMS Microbiol Lett (2004): 49

Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC
Authors: Hyrc KL, Bownik JM, Goldberg MP.
Journal: Cell Calcium (2000): 75

Characterization of Mg2+ transport in brush border membrane vesicles of rabbit ileum studied with mag-fura-2
Authors: Juttner R, Ebel H.
Journal: Biochim Biophys Acta (1998): 51

Intracellular Mg2+ regulation in voltage-clamped Helix aspersa neurones measured with mag-fura-2 and Mg(2+)-sensitive microelectrodes
Authors: Kennedy HJ.
Journal: Exp Physiol (1998): 449

Mag-Fura-2 (Furaptra) exhibits both low (microM) and high (nM) affinity for Ca2+
Authors: Martinez-Zaguilan R, Parnami J, Martinez GM.
Journal: Cell Physiol Biochem (1998): 158

Monitoring of Ca2+ release from intracellular stores in permeabilized rat parotid acinar cells using the fluorescent indicators Mag-fura-2 and calcium green C18
Authors: Tojyo Y, Tanimura A, Matsumoto Y.
Journal: Biochem Biophys Res Commun (1997): 189

Effects of monovalent cations, pH and temperature on the dissociation constant (KD) for the fluorescent indicator mag-fura-2 at different excitation wavelengths
Authors: Howarth FC, Singh J, Waring JJ, Hustler BI, Bailey M.
Journal: Magnes Res (1995): 299

Intrasynaptosomal free Mg2+ concentration measured with the fluorescent indicator mag-fura-2: modulation by Na+ gradient and by extrasynaptosomal ATP
Authors: Garcia-Martin E, Martin-Romero FJ, Gutierrez-Merino C.
Journal: J Neurochem (1995): 2757

Determination of cytosolic Mg2+ activity and buffering in BC3H-1 cells with mag-fura-2
Authors: Grubbs RD, Walter A.
Journal: Mol Cell Biochem (1994): 11

说明书
钙离子荧光探针Mag-Fura-2, AM CAS 132319-57-4.pdf