TaKaRa EpiTaq™ HS (for bisulfite-treated DNA)酶试剂盒Takara

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TaKaRa EpiTaq HS (for bisulfite-treated DNA)
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara R110Q TaKaRa EpiTaq HS (for bisulfite-treated DNA) 50 U ¥481 TaKaRa EpiTaq™ HS (for bisulfite-treated DNA) TaKaRa EpiTaq™ HS (for bisulfite-treated DNA) TaKaRa EpiTaq™ HS (for bisulfite-treated DNA)
Takara R110A TaKaRa EpiTaq HS (for bisulfite-treated DNA) 250 U ¥2,003 TaKaRa EpiTaq™ HS (for bisulfite-treated DNA) TaKaRa EpiTaq™ HS (for bisulfite-treated DNA) TaKaRa EpiTaq™ HS (for bisulfite-treated DNA)
Takara R110B (A × 4) TaKaRa EpiTaq HS (for bisulfite-treated DNA) 250 U × 4 ¥7,209 TaKaRa EpiTaq™ HS (for bisulfite-treated DNA) TaKaRa EpiTaq™ HS (for bisulfite-treated DNA) TaKaRa EpiTaq™ HS (for bisulfite-treated DNA)
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■ 制品内容 (Code No.: R110A)
TaKaRa EpiTaq HS (5 U/μl) 250 U
10 × EpiTaq PCR Buffer (Mg2+ free) 1 ml
dNTP Mixture (各2.5 mM) 1.2 ml
25 mM MgCl2 1.2 ml
 
 
■ 制品说明
TaKaRa EpiTaq HS (for bisulfite-treated DNA) 是用于亚硫酸氢盐修饰后含有尿嘧啶的模板DNA进行PCR扩增时所需的理想DNA聚合酶。亚硫酸氢盐修饰后的DNA有时会影响PCR反应性能,本制品中的镁离子及dNTP浓度经调整后可调节扩增效率和反应特异性,能够很好地扩增难扩增的目的片段。
本制品适用于Hot Start PCR,高温加热前,抗Taq单克隆抗体与Taq酶结合,抑制聚合酶的活性,从而抑制低温条件下由引物的非特异性退火或引物二聚体引起的非特异性扩增。抗体在PCR反应最初的DNA变性步骤已变性,因此无需特殊变性,在常规的PCR反应条件下即可使用。
 
■ 保存
-20℃。
 
 
Technote:
Superior amplification of bisulfite-treated DNA for methylation studies
 

页面更新:2022-07-29 13:39:28