ReadiUse 预活化 PE 马来酰亚胺 [活化 R-藻红蛋白] 货号2565-AAT Bioquest荧光染料

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ReadiUse 预活化 PE 马来酰亚胺 [活化 R-藻红蛋白]

ReadiUse 预活化 PE 马来酰亚胺 [活化 R-藻红蛋白]

货号 2565 存储条件 避免受潮, 在2-8度冷藏保存, 避免光照
规格 1 mg 价格 1140
Ex (nm) 566 Em (nm) 574
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:2565

产品名称:ReadiUse 预活化 PE 马来酰亚胺 [活化 R-藻红蛋白]

规格:1mg

储存条件:2-8℃避光防潮

保质期:6个月

 

产品物理化学光谱特性

溶剂:水

激发波长(nm):566

发射波长(nm):574

 

产品介绍

ReadiUse 预活化 PE 马来酰亚胺是通过高度纯化的 R-藻红蛋白 (PE) 与 SMCC 反应制备的。 SMCC 的 NHS 基团与 PE 的赖氨酸基团反应,留下马来酰亚胺基团可与待缀合的蛋白质上的游离巯基反应。 ReadiUse 预活化 PE 马来酰亚胺经过高度纯化,可完全去除未反应的 SMCC,并冻干成粉末形式。 它可以立即使用,并且在与含巯基的靶分子(如还原型 IgG)混合后无需进一步制备即可偶联。 这种活化的藻胆蛋白可以很容易地与抗体和其他蛋白质结合,而无需使用添加的化学交联剂。 制备的偶联物保持了 PE 的光谱特性。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的ReadiUse 预活化 PE 马来酰亚胺 [活化 R-藻红蛋白]。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

说明书
ReadiUse 预活化 PE 马来酰亚胺 [活化 R-藻红蛋白].pdf