Bcl10 (331.3)抗体 (HRP 标记) Bcl10 (331.3) HRP

Bcl10 (331.3)抗体 (HRP 标记)

Bcl10 (331.3) HRP

详细描述:
Bcl10, also designated CIPER, c-CARMEN and mE10, was first identified as a gene truncated or mutated in MALT B cell lymphomas and other tumor types. Bcl10 is homologous to the equine herpesvirus-2 E10 gene and, like E10, it contains an N-terminal caspase

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:HRP。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5273 HRP Bcl10 (331.3)抗体 (HRP 标记) 200 µg/ml 咨询客服

Bcl10 (331.3)抗体 (FITC 标记) Bcl10 (331.3) FITC

Bcl10 (331.3)抗体 (FITC 标记)

Bcl10 (331.3) FITC

详细描述:
Bcl10, also designated CIPER, c-CARMEN and mE10, was first identified as a gene truncated or mutated in MALT B cell lymphomas and other tumor types. Bcl10 is homologous to the equine herpesvirus-2 E10 gene and, like E10, it contains an N-terminal caspase

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:FITC。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5273 FITC Bcl10 (331.3)抗体 (FITC 标记) 200 µg/ml 咨询客服

Bcl10 (331.3)抗体 ( PE 标记) Bcl10 (331.3) PE

Bcl10 (331.3)抗体 ( PE 标记)

Bcl10 (331.3) PE

详细描述:
Bcl10, also designated CIPER, c-CARMEN and mE10, was first identified as a gene truncated or mutated in MALT B cell lymphomas and other tumor types. Bcl10 is homologous to the equine herpesvirus-2 E10 gene and, like E10, it contains an N-terminal caspase

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5273 PE Bcl10 (331.3)抗体 ( PE 标记) 200 µg/ml 咨询客服

PicoPLEX Single Cell WGA Kit v3酶试剂盒Takara

上海金畔生物科技有限公司代理Takara酶试剂盒全线产品,欢迎访问官网了解更多产品信息。

PicoPLEX Single Cell WGA Kit v3
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Clontech R300718 PicoPLEX Single Cell WGA Kit v3 24 Rxns ¥5,518 PicoPLEX Single Cell WGA Kit v3 PicoPLEX Single Cell WGA Kit v3 PicoPLEX Single Cell WGA Kit v3
Clontech R300722 PicoPLEX Single Cell WGA Kit v3 96 Rxns ¥19,606 PicoPLEX Single Cell WGA Kit v3 PicoPLEX Single Cell WGA Kit v3 PicoPLEX Single Cell WGA Kit v3
Clontech R300723 PicoPLEX Single Cell WGA Kit v3 480 Rxns ¥83,328 PicoPLEX Single Cell WGA Kit v3 PicoPLEX Single Cell WGA Kit v3 PicoPLEX Single Cell WGA Kit v3
收藏产品 加入购物车
 
 
PicoPLEX Single Cell WGA Kit v3
· 单细胞(或<15 pg DNA)起始,可获得可重复的结果
· 卓越的等位基因再现性
· 易于使用和自动化
· 应用广泛,可以用于微阵列,PCR等
 
PicoPLEX Single Cell WGA Kit v3 (PicoPLEX WGA v3)可以从单细胞扩增DNA,获得高度可重复的文库。细胞裂解和预扩增后,进行低背景的PCR扩增,并在3小时内获得微克级产物。
除了延续之前PicoPLEX WGA Kit可进行高度可重复的拷贝数变异(CNV)分析外,该试剂盒通过使用高保真酶,尽可能减少扩增误差,并能检测单核苷酸变异(SNV)。
 
PicoPLEX Single Cell WGA Kit v3
图1. PicoPLEX Single Cell WGA Kit v3技术
 
表1 .使用PicoPLEX Single Cell WGA Kit v3进行高质量单核苷酸变异(SNV)检测
PicoPLEX Single Cell WGA Kit v3
 
以单细胞 (1cell) 和5个细胞 (5 cells) 的GM12878细胞系 (Coriell研究所) 起始 (一式两份),分别使用PicoPLEX WGA v3,A公司试剂盒和B公司试剂盒,以及bulk gDNA对照制备全基因组扩增产物,进行SNV检测的比较。其中B公司试剂盒虽然制备了足够的扩增产物,但只有5 cells样本中一个包含足够的扩增子进行测序,而其他样本无可用数据进行测序。根据Genome In a Bottle (GIB) 和hg19 (human Genome assembly GRCh37, Ensembl) 数据库的交叉分析,预计GM12879细胞系总共存在78个SNVs。由于扩增子的设计,2×75 bp的读取长度很短,78个SNVs中的4个未能检测到。因此,检测的SNV总数减少到74个。使用VarDict软件,以≥10reads (10X coverage) 和等位基因频率≥20%为基准从BAM文件分析SNV。与其他两个公司试剂盒相比,PicoPLEX WGA v3具有更高的call rates和更低的等位基因丢失率。
(数据来源于Takara Bio USA, Inc.)
 
PicoPLEX Single Cell WGA Kit v3
图2. 基于PicoPLEX Single Cell WGA Kit v3的CNV检测
 
以来自不同细胞系(GM22601、GM05067和GM12878)的单细胞起始,使用PicoPLEX Single Cell WGA Kit v3扩增基因组。之后取1ng的扩增产物,使用Illumina Nextera XT Kit制备文库,并在Illumina MiSeq(PE 75)进行测序。对Fastq文件去除接头序列信息后,与human genome assembly GRCh37进行比对。只分析常染色体。对于这两个panels,校准归一化至100万reads,使用bedtools 2.25.0计算每1 Mb的 bin的reads数。使用Integrative Genomics Viewer (IGV)绘制bin counts的log2 ratio (Sample/Reference)。PicoPLEX Single Cell WGA Kit v3还显示了可以高品质的进行染色体异倍性分析。
 
PicoPLEX Single Cell WGA Kit v3
图3. PicoPLEX Single Cell WGA Kit v3的全基因组覆盖范围的重现性高
 
以GM12878的单细胞样本起始(一式两份),分别使用PicoPLEX WGA v3,A公司试剂盒和B公司试剂盒扩增基因组。之后取1ng的扩增产物,使用Illumina Nextera XT Kit制备文库,并在MiSeq(PE 75)进行测序。然后与human genome assembly GRCh37进行比对,归一化至1M reads (0.5M paired-end reads)后,使用bedtools 2.25.0计算每个1 Mb的bin的reads数。对这两个单细胞文库的每个窗口的total read进行绘制,计算Pearson和Spearman相关性并在每个图中标明。
Panel A. 包含异常值的样本。*由于不同技术存在不同的偏差,没有排除相关点,所以图表的比例不同。
Panel B. 排除异常值的样本。与A公司和B公司试剂盒相比,PicoPLEX WGA v3具有更高的重现性和覆盖度。
(数据来源于Takara Bio USA, Inc.)
 
 
Technote:PicoPLEX Single Cell WGA Kit v3
 
资料下载:PicoPLEX Single Cell WGA Kit v3
 
产品详情请点击:PicoPLEX Single Cell WGA Kit v3
 
 
 

页面更新:2020-09-01 09:47:03

Bcl10 (331.3)抗体(Alexa Fluor® 488 标记) Bcl10 (331.3) Alexa Fluor® 488

Bcl10 (331.3)抗体(Alexa Fluor® 488 标记)

Bcl10 (331.3) Alexa Fluor® 488

详细描述:
Bcl10, also designated CIPER, c-CARMEN and mE10, was first identified as a gene truncated or mutated in MALT B cell lymphomas and other tumor types. Bcl10 is homologous to the equine herpesvirus-2 E10 gene and, like E10, it contains an N-terminal caspase

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:AF488 。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5273 AF488 Bcl10 (331.3)抗体(Alexa Fluor® 488 标记) 200 µg/ml 咨询客服

Bcl10 (331.3)抗体( Alexa Fluor® 647 标记) Bcl10 (331.3) Alexa Fluor® 647

Bcl10 (331.3)抗体( Alexa Fluor® 647 标记)

Bcl10 (331.3) Alexa Fluor® 647

详细描述:
Bcl10, also designated CIPER, c-CARMEN and mE10, was first identified as a gene truncated or mutated in MALT B cell lymphomas and other tumor types. Bcl10 is homologous to the equine herpesvirus-2 E10 gene and, like E10, it contains an N-terminal caspase

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:AF647 。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5273 AF647 Bcl10 (331.3)抗体( Alexa Fluor® 647 标记) 200 µg/ml 咨询客服

β Tubulin (D-10)抗体 β Tubulin (D-10)

β Tubulin (D-10)抗体

β Tubulin (D-10)

详细描述:
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) h

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG2b; 标记:无标记。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5274 β Tubulin (D-10)抗体 200 µg/ml 咨询客服

β Tubulin (D-10)抗体(琼脂糖偶联) β Tubulin (D-10) AC

β Tubulin (D-10)抗体(琼脂糖偶联)

β Tubulin (D-10) AC

详细描述:
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) h

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG2b; 标记:Agarose。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5274 AC β Tubulin (D-10)抗体(琼脂糖偶联) 500 µg/ml, 25% ag 咨询客服

Calcium Standard Solution (Ca : 1,000mg/l) 钙标准溶液 (Ca 1,000) 品牌:Wako


品牌:Wako
CAS No.:N/A
储存条件:25℃以下
纯度:990~1010mg/l
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

039-16161

JCSS (Japan Calibration Service System) 100 ml 400.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

产品描述相关资料下载相关产品浏览记录 请联系客服

β Tubulin (D-10)抗体 (HRP 标记) β Tubulin (D-10) HRP

β Tubulin (D-10)抗体 (HRP 标记)

β Tubulin (D-10) HRP

详细描述:
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) h

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG2b; 标记:HRP。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5274 HRP β Tubulin (D-10)抗体 (HRP 标记) 200 µg/ml 咨询客服

β Tubulin (D-10)抗体 (FITC 标记) β Tubulin (D-10) FITC

β Tubulin (D-10)抗体 (FITC 标记)

β Tubulin (D-10) FITC

详细描述:
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) h

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG2b; 标记:FITC。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5274 FITC β Tubulin (D-10)抗体 (FITC 标记) 200 µg/ml 咨询客服

β Tubulin (D-10)抗体 ( PE 标记) β Tubulin (D-10) PE

β Tubulin (D-10)抗体 ( PE 标记)

β Tubulin (D-10) PE

详细描述:
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) h

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG2b; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5274 PE β Tubulin (D-10)抗体 ( PE 标记) 200 µg/ml 咨询客服

(2-Nitrophenyl)methyl 4-(Methacryloyloxy)piperidine-1-carboxylate (2-硝基苯基)甲基- 4-(2-甲基丙烯酰氧)哌啶-1-羧酸盐(WPBG-165) 品牌:Wako


品牌:Wako
CAS No.:1292812-05-5
储存条件:2-10℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

351-33733

5 g 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

产品描述相关资料下载相关产品浏览记录 请联系客服

β Tubulin (D-10)抗体(Alexa Fluor® 488 标记) β Tubulin (D-10) Alexa Fluor® 488

β Tubulin (D-10)抗体(Alexa Fluor® 488 标记)

β Tubulin (D-10) Alexa Fluor® 488

详细描述:
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) h

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG2b; 标记:AF488 。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5274 AF488 β Tubulin (D-10)抗体(Alexa Fluor® 488 标记) 200 µg/ml 咨询客服

β Tubulin (D-10)抗体( Alexa Fluor® 647 标记) β Tubulin (D-10) Alexa Fluor® 647

β Tubulin (D-10)抗体( Alexa Fluor® 647 标记)

β Tubulin (D-10) Alexa Fluor® 647

详细描述:
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) h

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG2b; 标记:AF647 。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5274 AF647 β Tubulin (D-10)抗体( Alexa Fluor® 647 标记) 200 µg/ml 咨询客服

CD63 (MX-49.129.5)抗体 CD63 (MX-49.129.5)

CD63 (MX-49.129.5)抗体

CD63 (MX-49.129.5)

详细描述:
The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 (also known as LAMP-3, Melanoma-associate

应用范围:WB, IP, IF, IHC(P), FCM, ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:无标记。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5275 CD63 (MX-49.129.5)抗体 200 µg/ml 咨询客服

CD63 (MX-49.129.5)抗体(琼脂糖偶联) CD63 (MX-49.129.5) AC

CD63 (MX-49.129.5)抗体(琼脂糖偶联)

CD63 (MX-49.129.5) AC

详细描述:
The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 (also known as LAMP-3, Melanoma-associate

应用范围:WB, IP, IF, IHC(P), FCM, ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:Agarose。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5275 AC CD63 (MX-49.129.5)抗体(琼脂糖偶联) 500 µg/ml, 25% ag 咨询客服

Sodium Standard Solution (Na 1000) 钠标准溶液(Na 1000) 品牌:Wako


品牌:Wako
CAS No.:N/A
储存条件:25℃以下
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

199-10831

JCSS (Japan Calibration Service System) 100 ml 390.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

产品描述相关资料下载相关产品浏览记录 请联系客服

CD63 (MX-49.129.5)抗体 (HRP 标记) CD63 (MX-49.129.5) HRP

CD63 (MX-49.129.5)抗体 (HRP 标记)

CD63 (MX-49.129.5) HRP

详细描述:
The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 (also known as LAMP-3, Melanoma-associate

应用范围:WB, IP, IF, IHC(P), FCM, ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:HRP。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5275 HRP CD63 (MX-49.129.5)抗体 (HRP 标记) 200 µg/ml 咨询客服

CD63 (MX-49.129.5)抗体 (FITC 标记) CD63 (MX-49.129.5) FITC

CD63 (MX-49.129.5)抗体 (FITC 标记)

CD63 (MX-49.129.5) FITC

详细描述:
The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 (also known as LAMP-3, Melanoma-associate

应用范围:WB, IP, IF, IHC(P), FCM, ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:FITC。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-5275 FITC CD63 (MX-49.129.5)抗体 (FITC 标记) 200 µg/ml 咨询客服