组成

状态

容量

(A) 抗体固相化   96 孔板

洗净后使用

96 wells（8×12）/1 块

(B) 标准溶液（6,000 pg/mL）

稀释后使用

500 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D) 抗C-肽抗体生物素结合

稀释后使用

100 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

100 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1 瓶

(H) 反应停止液（1M   H₂SO₄）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

4 张

使用说明书

1 份

样本

A

B

1

976

238

2

969

230

3

965

230

4

1023

235

5

977

231

6

1018

228

7

1038

229

8

995

225

mean

995

231

SD

27.7

4.10

CV(%)

2.78

1.78

测量日/检体

C

D

E

第0天

1502

301

60.9

第1天

1500

302

63.8

第2天

1499

301

62.2

第3天

1501

300

58.8

mean

1500

301

61.4

SD

1.12

0.66

2.13

CV(%)

0.07

0.22

3.46

添加量

实测值

回收量

回收率(%)

0.00

300

–

–

265

551

250

94

398

683

382

96

531

827

527

99

添加量

实测值

回收量

回收率(%)

0.00

58.2

–

–

28.9

86.7

28.5

99

38.6

98.2

40.0

104

77.4

139

80.8

104

ELISA试剂盒选择指南①②

 1.

Inhibition of Gastric Inhibitory Polypeptide Receptor Signaling in Adipose Tissue Reduces Insulin Resistance and Hepatic Steatosis in High-Fat Diet-Fed Mice. Joo E, Harada N, Yamane S, Fukushima T, Taura D, Iwasaki K, Sankoda A, Shibue K, Harada T, Suzuki K, Hamasaki A, Inagaki N. Diabetes. 2017 Apr;66(4):868-879.

 2.

Inhibition of Gastric Inhibitory Polypeptide Receptor Signaling in Adipose Tissue Reduces Insulin

Resistance and Hepatic Steatosis in High Fat Diet-Fed Mice. Joo E, Harada N, Yamane S, Fukushima

T, Taura D, Iwasaki K, Sankoda A, Shibue K, Harada T, Suzuki K, Hamasaki A, Inagaki N. Diabetes.

2017 Jan 17.

 3.

Insulin Release from the Beta Cells in Acatalasemic Mice Is Highly Susceptible to Alloxan-Induced Oxidative Stress. Kazunori Takemoto, Wakana Doi, Ken Kataoka, Kohji Ishihara, Da-Hong Wang, Hitoshi Sugiyama, Noriyoshi Masuoka. Journal of Diabetes Mellitus, 2015, 5, 81-89

 4.

Effect of Burdock Root and the Fermented Product on Alloxan-Induced Mouse Hyperglycemia

Wakana Doi, Yumi Asada, Ayaka Ohno, Yoshiko Okuda, Shota Masuda, Ayano Matsumoto,

Chihiro Mori, Takaya Agarie, Kohji Ishihara, Takayuki Murakami & Noriyoshi Masuoka Journal of

Food Research; Vol. 4, No. 4; 201

 5.

Tissue Complex of Adult Pancreatic Duct and Vascular Endothelial Cells Promotes In Vitro Differentiation into Insulin-Producing Cells. Jun Kanamune, Chongmun Kim, Yasuhiro Iwanaga, Jorge David Rivas-Carrillo, Shoichiro Sumi, Shinji Uemoto and Kazuyuki Yokokawa. J Stem Cell Res Dev 2015, 2: 005

 6.

Anti-diabetic effect of purple corn extract on C57BL/KsJ db/db mice. Bo Huang, Zhiqiang Wang, Jong Hyuk Park, Ok Hyun Ryu, Moon Ki Choi, Jae-Yong Lee, Young-Hee Kang, and Soon Sung Lim. Nutr Res Pract. 2015 Feb;9(1):22-29.

 7.

Xanthohumol Improves Diet-induced Obesity and Fatty Liver by Suppressing Sterol Regulatory Element-binding Protein (SREBP) Activation. Miyata S, Inoue J, Shimizu M, Sato R. J Biol Chem. 2015 Aug 14;290(33):20565-79.

 8.

Effect of Aspergillus awamori-Fermented Burdock Root on Mouse Diabetes Induced by

Alloxan—Prevention of Cell Apoptosis. Kazunori Takemoto, Wakana Doi, Ayumi Zukeran, Junji Inoue, Kohji Ishihara, Noriyoshi Masuoka. Food and Nutrition Sciences, Vol.5 No.16(2014), Article ID:49228,7 pages

 9.

Engineering of pseudoislets: effect on insulin secretion activity by cell number, cell population, and microchannel networks. Kojima N, Takeuchi S, Sakai Y. Transplant Proc. Vol.46(4), p1161-65, May 2014.

10.

Evaluation of 7-O-galloyl-d-sedoheptulose, isolated from Corni Fructus, in the adipose tissue of type 2 diabetic db/db mice. Park CH., Tanaka T., Yokozawa T. Fitoterapia, Vol.89, p131-142, Sep 2013.

11.

Periaortic adipose tissue-specific activation of the renin-angiotensin system contributes to atherosclerosis development in uninephrectomized apoE-/- mice. Kawahito H., Yamada H., Irie D., Kato T., Akakabe Y., Kishida S., Takata H., Wakana N., Ogata T., Ikeda K., Ueyama T., Matoba S., Mori Y., Matsubara H. American Journal of Physiology – Heart and Circulatory Physiology, Vol.305, p667-675, Sep 2013.

12.

Effect of vitamin E on alloxan-induced mouse diabetes. Kamimura W., Doi W., Takemoto K., Ishihara K., Wang D-H., Sugiyama H., Oda S., Masuoka N. Clinical Biochemistry, Vol.46(9), p795-798, Jun 2013.

13.

Evaluation of 7-O-galloyl-d-sedoheptulose, isolated from Corni Fructus, in the adipose tissue of type 2 diabetic db/db mice. C.H.Park, T.Tanaka, T.Yokozawa. Fitoterapia,  Vol.89, p131-42, Sep 2013.

14.

Therapeutic approach for type 1 diabetes mellitus using the novel immunomodulator FTY720 (fingolimod) in combination with once-daily injection of insulin glargine in non-obese diabetic mice. T.Tsuji, M.Inoue, Y.Yoshida, T.Fujita, Y.Kaino, T.Kohno. Journal of Diabetes Investigation, Vol.3(2), p132-137, Apr 2012.

15.

Effect of vitamin E on alloxan-induced mouse diabetes. Kamimura W, Doi W, Takemoto K, Ishihara K, Wang D-H, Sugiyama H, Oda S, Masuoka N. Clinical Biochemistry, Mar 2013.

16.

Intramedullary Cavity as an Implant Site for Bioartificial Pancreas: An In Vivo Study on Diabetic Canine. Y, Kai-Chiang., W, Chang-Chin., S, Shoichiro., K, Tzong-Fu., L, Sheng-Chuan., L, Feng-Huei. Transplantation, Vol. 90(6), p604-611, Sep 2010.

17.

The in vivo performance of bioartificial pancreas in bone marrow cavity: A case report of a spontaneous diabetic feline. K, C, Yang., C, C, Wu., S, C, Lin., S, Sumi., F, H, Lin. Biochemical and Biophysical Research Communications, Vol.393(3), p362-364, Mar 2010.

18.

In vitro reprogramming of adult hepatocytes into insulin-producing cells without viral vectors . H, Motoyama., S, Ogawa., A, Kubo., S, Miwa., J, Nakayama., Y, Tagawa., S, Miyagawa. Biochemical and Biophysical Research Communications, Vol.385(1),  p123-128, Jul 2009.

19.

Efficient differentiation of insulin-producing cells from skin-derived stem cells. Guo,W.,Miao,C.,Liu,S.,Qiu,Z.,Li,J., and Duan, E. Cell Proliferation, Vol.42(1), p49-62, 2009.

20.

Enrichment of Putative Pancreatic Progenitor Cells From Mice by Sorting for Prominin1(CD133)and PDGFRb. Yuichi Hori,Miki Fukomoto,Yoshikazu Kuroda. Stem Cells;0:2008-0192v1,2008

21.

Possibility of insulin-producing cells derived from mouse embryonic stem cells for diabetes treatment. T, Ibii., H, Shimada., S, Miura., E, Fukuma., H, Sato., H, Iwata. Journal of Bioscience and Bioengineering,Vol.103(2), p140-146, Feb 2007.

22.

The dual function of hepatic SOCS3 in insulin resistance in vivo. Torisu, T., Sato, N., Yoshiga, D., Kobayashi, T., Yoshioka, T., Mori, H., Iida, M. and Yoshimura, A. Genes to Cells, 12, p143-154, 2007.

23.

Prolonged remission of diabetes by regeneration of bold italic beta cells in diabetic mice treated with recombinant adenoviral vector expressing glucagon-like peptide-1. Liu, M.J., Shin, S., Li, N., Shigehara, T., Lee, Y.S., Yoon, J.W., and Jun H.S. Molecular Therapy 15: p86-93, 2007.

24.

Possibility of insulin-producing cells derived from mouse embryonic stem cells for diabetes treatment. Ibii, T., Shimada, H, Miura, S., Fukuma, E.,Sato, H.,and Iwata,H. J Bioscience Bioengineering 103: p140-146, 2007.

25.

A human b-cell line for transplantation therapy to control type 1 diabetes. Narushima, M., Kobayashi, N., Okitsu, T., Tanaka, Y., Li, S.A., Chen, Y., Miki, A., Tanaka, K., Nakaji, S., Takei, K., Gutierrez, A.S., Rivas-Carrillo, J.D., Navarro-Alvarez, N., Jun, H.S., Westerman, K.A., Noguchi, H., Lakey, J.R.T.,, Leboulch, P., Tanaka, N., and Yoon, J.W. Nature Biotechnology, Vol. 23(10), p1274-1282, 2005.

组成

状态

容量

(A)   抗体固相化 96 孔板

洗净后使用

96   wells（8×12）/1 块

(B)   胰岛素标准溶液（大鼠）（200 ng/mL）

稀释后使用

300 μL/1 瓶

(C)   缓冲液（蓝色）

即用

60 mL/1 瓶

(D)  生物素结合抗胰岛素抗体

稀释后使用

200 μL/1 瓶

(E)   过氧化物・抗生物素蛋白结合物

稀释后使用

200 μL/1 瓶

(F)   显色液（TMB）

即用

12 mL/1 瓶

(H)  反应终止液（1M   H₂SO₄）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

样品

A

B

C

1

2.73

12.9

86.7

2

2.78

12.6

86.2

3

2.78

12.8

85.6

4

2.78

12.6

85.2

5

2.73

12.6

85.9

mean

2.76

12.7

85.9

SD

0.025

0.147

0.575

CV(%)

0.9

1.2

0.7

测量日/样品

D

E

F

第０天

3.34

25.5

70.3

第１天

3.28

25.7

70.3

第２天

3.15

25.2

71.6

mean

3.26

25.5

70.7

SD

0.097

0.280

0.765

CV(%)

3.0

1.1

1.1

添加量

实测值

回收量

回收率(%)

0.00

31.3

–

–

20.0

51.2

19.9

99.5

40.0

73.1

41.8

105

60.0

94.3

63.0

105

添加量

实测值

回收量

回收率(%)

0.00

1.36

–

–

1.21

2.58

1.22

101

2.46

3.75

2.39

97.2

3.46

4.64

3.28

94.8

ELISA试剂盒选择指南①②

1.

Duodenal-jejunal bypass improves diabetes and liver steatosis via enhanced glucagon-like peptide-1 elicited by bile acids. Kashihara H, Shimada M, Kurita N, Sato H, Yoshikawa K, Higashijima J, Chikakiyo M, Nishi M, Takasu C. J Gastroenterol Hepatol. Vol.30(2), p308-15, Feb 2015.

2.

Improving Effects of Narazuke Lees on Fatty Liver of Rats Induced by High-Fat and High-Cholesterol Diets. Nakasa T, Yamagami S, Tanaka T, Tanaka H, Hariu H, Okinaka O. Food Science and Technology Research, Vol.20 (2014), No. 4, p849-857.

3.

Duodenal-jejunal bypass improves diabetes and liver steatosis via enhanced glucagon-like peptide-1 elicited by bile acids. Kashihara H, Shimada M, Kurita N, Sato H, Yoshikawa K, Higashijima J, Chikakiyo M, Nishi M, Takasu C. J Gastroenterol Hepatol. Aug 2014.

4.

Effect of diets with different fat contents on the development of diabetes in female Zucker diabetic fatty rat with leptin mutation. Kohlerova, Renata; Sznapkova, Martina; Slavkovsky, Rastislav; Jiroutova, Alena. Acta Veterinaria Brno, Vol.82(3), p289-296, 2013.

5.

Regulation of oxidative stress and inflammation by hepatic adiponectin receptor 2 in an animal model of nonalcoholic steatohepatitis. Matsunami,T.,Sato,Y.,Ariga,S.,Sato,T.,Kashimura,H.,Hasegawa,Y.,Yukawa, M. Int J Clin Exp Pathol. Vol.3(5), p472-481, 2010.

组成

状态

容量

(A) 抗体固相化 96 孔板

洗净后使用

96   wells（8×12）/1 块

(B) 胰岛素标准溶液（大鼠）（200 ng/mL）

稀释后使用

25 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D) 生物素结合抗胰岛素抗体

稀释后使用

10 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

20 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1 瓶

(H) 反应终止液（1M H₂SO₄）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

样品

A

B

C

D

1

0.589

1.211

2.600

4.991

2

0.568

1.228

2.600

4.971

3

0.568

1.228

2.532

5.036

4

0.557

1.211

2.538

5.026

5

0.557

1.253

2.582

4.925

6

0.578

1.220

2.563

4.880

7

0.578

1.228

2.618

5.031

8

0.536

1.228

2.618

4.885

mean

0.566

1.226

2.581

4.968

SD

0.0165

0.0131

0.0340

0.0645

CV(%)

2.92

1.07

1.32

1.30

测量日/样品

E

F

G

第０天

6.74

3.31

1.16

第１天

6.69

3.25

1.22

第２天

6.23

3.21

1.21

mean

6.55

3.25

1.20

SD

0.2792

0.0479

0.0325

CV(%)

4.3

1.5

2.7

添加量

理论值

实测值

回收率(%)

0

–

0.996

–

0.500

1.496

1.484

99.2

1.000

1.996

2.048

103

2.000

2.996

2.779

92.7

添加量

理论值

实测值

回收率(%)

0

–

1.086

–

0.500

1.586

1.562

98.5

1.000

2.086

2.061

98.8

2.000

3.086

2.753

89.2

添加量

理论值

实测值

回收率(%)

0

–

1.160

–

0.500

1.660

1.637

98.6

1.000

2.160

2.054

95.1

2.000

3.166

2.963

93.6

ELISA试剂盒选择指南①②

 1.

Canagliflozin potentiates GLP-1 secretion and lowers the peak of GIP secretion in rats fed a high-fat high-sucrose diet. Hira T, Koga T, Sasaki K, Hara H. Biochem Biophys Res Commun. 2017 Oct 14;492(2):161-165.

 2.

Imidacloprid insecticide exposure induces stress and disrupts glucose homeostasis in male rats. Khalil SR, Awad A, Mohammed HH, Nassan MA. Environ Toxicol Pharmacol. 2017 Oct;55:165-174.

 3.

Effects of short-term fasting on the Akt-mediated pathway involved in protein metabolism in chicken skeletal muscle. Saneyasu T, Tsuchii N, Nakano Y, Kitashiro A, Tsuchihashi T, Shindo H, Honda K, Kamisoyama H. Domest Anim Endocrinol. 2017 Oct;61:54-61.

 4.

Impact of difructose anhydride III, raffinose, and fructooligosaccharides on energy intake, gut hormones, and cecal fermentation in rats fed a high-fat and high-sucrose diet. Hira T, Yanagihara K, Koga T, Takahashi K, Nagura T, Uchino H, Hara H. Biosci Biotechnol Biochem. 2017 Sep 27:1-9.

 5.

Branched-chain amino acid supplementation restores reduced insulinotropic activity of a low-protein diet through the vagus nerve in rats. Horiuchi M, Takeda T, Takanashi H, Ozaki-Masuzawa Y, Taguchi Y, Toyoshima Y, Otani L, Kato H, Sone-Yonezawa M, Hakuno F, Takahashi SI, Takenaka A. Nutr Metab (Lond). 2017 Sep 15;14:59.

 6.

Role of Glyceraldehyde-derived AGEs and Mitochondria in Superoxide Production in Femoral Artery of OLETF Rat and Effects of Pravastatin. Hori E, Kikuchi C, Nagami C, Kajikuri J, Itoh T, Takeuchi M, Matsunaga T. Biol Pharm Bull. 2017 Aug 22.

 7.

Glucagon-like peptide-1 reduces pancreatic β-cell mass through hypothalamic neural pathways in high-fat diet-induced obese rats. Ando H, Gotoh K, Fujiwara K, Anai M, Chiba S, Masaki T, Kakuma T, Shibata H. Sci Rep. 2017 Jul 17;7(1):5578.

 8.

Beneficial effect of combined treatment with octreotide and pasireotide in PCK rats, an orthologous model of human autosomal recessive polycystic kidney disease. Kugita M, Nishii K, Yamaguchi T, Suzuki A, Yuzawa Y, Horie S, Higashihara E, Nagao S PLoS One. 2017 May 18;12(5):e0177934.

 9.

Green tea extract intake during lactation modified cardiac macrophage infiltration and AMP-activated protein kinase phosphorylation in weanling rats from undernourished mother during gestation and lactation. Matsumoto E, Kataoka S, Mukai Y, Sato M, Sato S. J Dev Orig Health Dis. 2017 Apr;8(2):178-187.

10.

Exogenous thyroxine improves glucose intolerance in insulin-resistant rats. Vazquez-Anaya G, Martinez B, Sonanez-Organis JG, Nakano D, Nishiyama A, Ortiz RM. J Endocrinol. 2017 Mar;232(3):501-511.

11.

Beneficial effects of metformin on energy metabolism and visceral fat volume through a possible mechanism of fatty acid oxidation in human subjects and rats. Tokubuchi I, Tajiri Y, Iwata S, Hara K, Wada N, Hashinaga T, Nakayama H, Mifune H, Yamada K PLoS One. 2017 Feb 3;12(2):e0171293.

12.

Resistant maltodextrin or fructooligosaccharides promotes GLP-1 production in male rats fed a high-fat and high-sucrose diet, and partially reduces energy intake and adiposity. Hira T, Suto R, Kishimoto Y, Kanahori S, Hara H. Eur J Nutr. 2017 Feb 4.

13.

Lactoferrin ameliorates corticosterone-related acute stress and hyperglycemia in rats. Maekawa Y. Sugiyama A, Takeuchi T. J Vet Med Sci. 2017 Feb 28;79(2):412-417.

14.

Dioscorea esculenta-induced increase in muscle sex steroid hormones is associated with enhanced insulin sensitivity in a type 2 diabetes rat model. Sato K, Fujita S, Iemitsu M. FASEB J. 2017 Feb;31(2):793-801.

15.

Mild Hyperbaric Oxygen Inhibits Growth-related Decrease in Muscle Oxidative Capacity of Rats with Metabolic Syndrome. Takemura A, Ishihara A. J Atheroscler Thromb. 2017 Jan 1;24(1):26-38.

16.

The Sodium Glucose Cotransporter 2 Inhibitor Ipragliflozin Promotes Preferential Loss of Fat Mass in Non-obese Diabetic Goto?Kakizaki Rats. Toshiyuki Takasu,  Yuka Hayashizaki,  Jiro Hirosumi,  Hideaki Minoura,  Nobuaki Amino,  Eiji Kurosaki,  Shoji Takakura. Biological and Pharmaceutical Bulletin, Vol.40 (2017), No. 5, p.675-680

17.

Anti-Prediabetic Effect of 6-O-Caffeoylsophorose in Prediabetic Rats and Its Stimulation of Glucose Uptake in L6 Myotubes. Gonzalo Miyagusuku-Cruzado,  Naoki Morishita,  Keiichi Fukui,  Norihiko Terahara,  Toshiro Matsui. Food Science and Technology Research, Vol.23(2017),  No.3, p.449-456

18.

The Sodium Glucose Cotransporter 2 Inhibitor Ipragliflozin Promotes Preferential Loss of Fat Mass in Non-obese Diabetic Goto-Kakizaki Rats. Takasu T, Hayashizaki Y, Hirosumi J, Minoura H, Amino N, Kurosaki E, Takakura S. Biol Pharm Bull. 2017;40(5):675-680.

19.

Evaluation of the Effects and Mechanism of L-Citrulline on Anti-obesity by Appetite Suppression in Obese/Diabetic KK-Ay Mice and High-Fat Diet Fed SD Rats. Kudo M, Yoshitomi H, Momoo M, Suguro S, Yamagishi Y, Gao M. Biol Pharm Bull. 2017;40(4):524-530.

20.

Exogenous thyroxine improves glucose intolerance in insulin-resistant rats. Vazquez-Anaya G, Martinez B, Sonanez-Organis JG, Nakano D, Nishiyama A, Ortiz RM. J Endocrinol. 2017 Mar;232(3):501-511

21.

Anti-prediabetic effect of rose hip (Rosa canina) extract in spontaneously diabetic Torii rats. Chen SJ, Aikawa C, Yoshida R, Kawaguchi T, Matsui T. J Sci Food Agric. 2017 Feb 9.

22.

O leuropein aglycone enhances UCP1 expression in brown adipose tissue in high-fat-diet-induced obese rats by activating β-adrenergic signaling. Oi-Kano Y, Iwasaki Y, Nakamura T, Watanabe T, Goto T, Kawada T, Watanabe K, Iwai K. J Nutr Biochem. 2017 Feb;40:209-218.

23.

Beneficial effects of metformin on energy metabolism and visceral fat volume through a possible mechanism of fatty acid oxidation in human subjects and rats. Tokubuchi I, Tajiri Y, Iwata S, Hara K, Wada N, Hashinaga T, Nakayama H, Mifune H, Yamada K. PLoS One. 2017 Feb 3;12(2):e0171293

24.

Resistant maltodextrin or fructooligosaccharides promotes GLP-1 production in male rats fed a high-fat and high-sucrose diet, and partially reduces energy intake and adiposity. Hira T, Suto R, Kishimoto Y, Kanahori S, Hara H. Eur J Nutr. 2017 Feb 4.

25.

Lactoferrin potentially facilitates glucose regulation and enhances the incretin effect. Maekawa Y， Sugiyama A, Takeuchi T. Biochem Cell Biol. 2017 Feb;95(1):155-161.

26.

Mild Hyperbaric Oxygen Inhibits Growth-related Decrease in Muscle Oxidative Capacity of Rats with Metabolic Syndrome. Takemura A, Ishihara A. J Atheroscler Thromb. 2017 Jan 1;24(1):26-38.

27.

Intracellular alkalinization by phosphate uptake via type III sodium-phosphate cotransporter participates in high-phosphate-induced mitochondrial oxidative stress and defective insulin secretion. Nguyen TT, Quan X, Xu S, Das R, Cha SK, Kong ID, Shong M, Wollheim CB, Park KS. FASEB J. 2016 Dec;30(12):3979-3988

28.

Plekhs1 and Prdx3 are candidate genes responsible for mild hyperglycemia associated with obesity in a new animal model of F344-fa-nidd6 rat. Kotoh J, Sasaki D, Matsumoto K, Maeda A. J Vet Med Sci. 2016 Dec 1;78(11):1683-1691.

29.

Melinjo (Gnetum gnemon) extract intake during lactation stimulates hepatic AMP-activated protein kinase in offspring of excessive fructose-fed pregnant rats. Kataoka S, Mukai Y, Takebayashi M, Kudo M, Acuram UR, Kurasaki M, Sato S. Reprod Biol. 2016 Jun;16(2):165-73

30.

Rice (Oryza sativa japonica) Albumin Suppresses the Elevation of Blood Glucose and Plasma Insulin Levels after Oral Glucose Loading. Ina S, Ninomiya K, Mogi T, Hase A, Ando T, Matsukaze N, Ogihara J, Akao M, Kumagai H, Kumagai H. J Agric Food Chem. 2016 Jun 22;64(24):4882-90.

31.

PPARγ activation alters fatty acid composition in adipose triglyceride, in addition to proliferation of small adipocytes, in insulin resistant high-fat fed rats. Sato D, Oda K, Kusunoki M, Nishina A, Takahashi K, Feng Z, Tsutsumi K, Nakamura T. Eur J Pharmacol. 2016 Feb 15;773:71-7.

32.

Exercise and dietary change ameliorate high fat diet induced obesity and insulin resistance via mTOR signaling pathway. Bae JY, Shin KO, Woo J, Woo SH, Jang KS, Lee YH, Kang S. J Exerc Nutrition Biochem. 2016 Jun;20(2):28-33.

33.

The IGF-1/Akt/S6 Signaling Pathway is Age-Dependently Downregulated in the Chicken Breast Muscle. Takaoki Saneyasu, Mariko Inui, Sayaka Kimura, Yu Yoshimoto, Nami Tsuchii, Haruka Shindo, Kazuhisa Honda, Hiroshi Kamisoyama. The Journal of Poultry Science, Article ID: 0150171

34.

The dipeptidyl peptidase IV inhibitor vildagliptin suppresses development of neuropathy in diabetic rodents: Effects on peripheral sensory nerve function, structure and molecular changes. Tsuboi K, Mizukami H, Inaba W, Baba M, Yagihashi S. J Neurochem. Vol.136, Issue 4, Feb. 2016, p859–870

35.

Characterization of bioactive agents in five types of marketed sprouts and comparison of their antihypertensive, antihyperlipidemic, and antidiabetic effects in fructose-loaded SHRs. Nakamura K, Koyama M, Ishida R, Kitahara T, Nakajima T, Aoyama T. J Food Sci Technol. 2016 Jan;53(1):581-90.

36.

Beneficial effect of D-allose for isolated islet culture prior to islet transplantation. Kashiwagi H, Asano E, Noguchi C, Sui L, Hossain A, Akamoto S, Okano K, Tokuda M, Suzuki Y. J Hepatobiliary Pancreat Sci. 2016 Jan;23(1):37-42.

37.

Characterization of bioactive agents in five types of marketed sprouts and comparison of their antihypertensive, antihyperlipidemic, and antidiabetic effects in fructose-loaded SHRs. Nakamura K, Koyama M, Ishida R, Kitahara T, Nakajima T, Aoyama T. J Food Sci Technol. 2016 Jan;53(1):581-90.

38.

Characterization of the Prediabetic State in a Novel Rat Model of Type 2 Diabetes, the ZFDM Rat. Gheni G, Yokoi N, Beppu M, Yamaguchi T, Hidaka S, Kawabata A, Hoshino Y, Hoshino M, Seino S. J Diabetes Res. 2015:261418.

39.

The dipeptidyl peptidase IV inhibitor vildagliptin suppresses development of neuropathy in diabetic rodents: Effects on peripheral sensory nerve function, structure and molecular changes. Tsuboi K, Mizukami H, Inaba W, Baba M, Yagihashi S. J Neurochem. 2015 Nov 25.

40.

Resistant maltodextrin promotes fasting glucagon-like peptide-1 secretion and production together with glucose tolerance in rats. Hira T, Ikee A, Kishimoto Y, Kanahori S, Hara H. Br J Nutr. Vol.114(1), p34-42, Jul 2015.

41.

Rice protein hydrolysates stimulate GLP-1 secretion, reduce GLP-1 degradation, and lower the glycemic response in rats. Ishikawa Y, Hira T, Inoue D, Harada Y, Hashimoto H, Fujii M, Kadowaki M, Hara H. Food Funct. Jun 2015.

42.

L-Citrulline increases hepatic sensitivity to insulin by reducing the phosphorylation of serine 1101 in insulin receptor substrate-1. Yoshitomi H, Momoo M, Ma X, Huang Y, Suguro S, Yamagishi Y, Gao M. BMC Complement Altern Med. Vol.15:188, Jun 2015.

43.

Black soybean extract reduces fatty acid contents in subcutaneous, but not in visceral adipose triglyceride in high-fat fed rats Sato D, Kusunoki M, Seino N, Nishina A, Feng Z, Tsutsumi K, Nakamura T. International Journal of Food Sciences and Nutrition , Published online: 20 May 2015.

44.

Fasting for 3 days during the suckling-weaning transient period in male rats induces metabolic abnormalities in the liver and is associated with impaired glucose tolerance in adulthood. Ikeda M, Honma K, Mochizuki K, Goda T. Eur J Nutr. May 2015.

45.

Stimulatory effect of insulin on renal proximal tubule sodium transport is preserved in type 2 diabetes with nephropathy. Nakamura M, Satoh N, Suzuki M, Kume H, Homma Y, Seki G, Horita S. Biochem Biophys Res Commun. Vol.461(1), p154-8, May 2015.

46.

Preserved Na/HCO3 cotransporter sensitivity to insulin may promote hypertension in metabolic syndrome. Nakamura M, Yamazaki O, Shirai A, Horita S, Satoh N, Suzuki M, Hamasaki Y, Noiri E, Kume H, Enomoto Y, Homma Y, Seki G. Kidney Int. Vol.87(3), p535-42, Mar 2015.

47.

Dietary protein derived from dried bonito fish improves type-2 diabetes mellitus-induced bone frailty in Goto-Kakizaki rats. Ochiai M, Kuroda T, Gohtani S, Matsuo T. J Food Sci. Vol.80(4), p848-56, Apr 2015.

48.

Ability of natural astaxanthin from shrimp by-products to attenuate liver oxidative stress in diabetic rats. Sila A, Kamoun Z, Ghlissi Z, Makni M, Nasri M, Sahnoun Z, Nedjar-Arroume N, Bougatef A. Pharmacol Rep. Vol.67(2), p310-6. Apr 2015.

49.

The effects of black garlic (Allium satvium) extracts on lipid metabolism in rats fed a high fat diet. Ha AW, Ying T, Kim WK. Nutr Res Pract. 2015 Feb;9(1):30-6.

50.

Resistant maltodextrin promotes fasting glucagon-like peptide-1 secretion and production together with glucose tolerance in rats. Hira T, Ikee A, Kishimoto Y, Kanahori S, Hara H. Br J Nutr. Vol.11:1-9. Feb 2015.

51.

 Effects of sleeve gastrectomy and gastric banding on the hypothalamic feeding center in an obese rat model. Kawasaki T, Ohta M, Kawano Y, Masuda T, Gotoh K, Inomata M, Kitano S. Surg Today. Feb 2015.

52.

Preventive effects of the angiotensin-converting enzyme inhibitor, captopril, on the development of azoxymethane-induced colonic preneoplastic lesions in diabetic and hypertensive rats. Kochi T, Shimizu M, Ohno T, Baba A, Sumi T, Kubota M, Shirakami Y, Tsurumi H, Tanaka T, Moriwaki H. Oncol Lett. Vol.8(1), p223-229. Jul 2014.

53.

Dietary Protein Derived from Dried Bonito Fish Improves Type-2 Diabetes Mellitus-Induced Bone Frailty in Goto-Kakizaki Rats. Ochiai M1, Kuroda T, Gohtani S, Matsuo T. J Food Sci. Feb 2015.

54.

Pancreatic fat accumulation, fibrosis, and acinar cell injury in the Zucker diabetic fatty rat fed a chronic high-fat diet. Matsuda A, Makino N, Tozawa T, Shirahata N, Honda T, Ikeda Y, Sato H, Ito M, Kakizaki Y, Akamatsu M, Ueno Y, Kawata S. Pancreas. Vol.43(5), p735-743. Jul 2014.

55.

Comparison of mechanisms underlying changes in glucose utilization in fasted rats anesthetized with propofol or sevoflurane: Hyperinsulinemia is exaggerated by propofol with concomitant insulin resistance induced by an acute lipid load. Li X, Kitamura T, Kawamura G, Mori Y, Sato K, Araki Y, Sato R, Yamada Y. Biosci Trends.Vol.8(3), p155-162. Jun 2014.

56.

Chronic Administration of Bovine Milk-Derived α-Lactalbumin Improves Glucose Tolerance via Enhancement of Adiponectin in Goto-Kakizaki Rats with Type 2 Diabetes. Yamaguchi M., Takai S. Biological and Pharmaceutical Bulletin, Vol. 37 (2014) No. 3, p.404-408, 2014.

57.

Maternal fructose intake during pregnancy modulates hepatic and hypothalamic AMP-activated protein kinase signaling in gender-specific manner in offspring. Mukai Y., Ozaki H., Serita Y. and Sato S. Clinical and Experimental Pharmacology and Physiology, 2014.

58.

SGLT2 selective inhibitor ipragliflozin reduces body fat mass by increasing fatty acid oxidation in high-fat diet-induced obese rats. Yokono M., Takasu T., Hayashizaki Y., Mitsuoka K., Kihara R., Muramatsu Y., Miyoshi S., Tahara A, Kurosaki E., Li Q., Tomiyama H., Sasamata M., Shibasaki M., Uchiyama Y. European Journal of Pharmacology, Vol.727, p66-74, Mar 2014.

59.

Non-alcoholic steatohepatitis and preneoplastic lesions develop in the liver of obese and hypertensive rats: suppressing effects of EGCG on the development of liver lesions. Kochi T, Shimizu M, Terakura D, Baba A, Ohno T, Kubota M, Shirakami Y, Tsurumi H, Tanaka T, Moriwaki H. Cancer Lett. Vol.342(1), p60-69, Jan 2014.

60.

Novel GPR40 agonist AS2575959 exhibits glucose metabolism improvement and synergistic effect with sitagliptin on insulin and incretin secretion. Tanaka H., Yoshida S., Minoura H., Negoro K., Shimaya A., Shimokawa T., Shibasaki M. Life Sciences, Vol.94(2), p115-121, Jan 2014.

61.

Taurine Alleviates the Progression of Diabetic Nephropathy in Type 2 Diabetic Rat Model. Koh JH., Lee ES., Hyun M., Kim HM., Choi YJ., Lee EY., Yadav D. and Chung CH. International Journal of Endocrinology, Vol.2014 (2014).

62.

 Effects of Sleeve Gastrectomy on Lipid Metabolism in an Obese Diabetic Rat Model. Kawano, Y., Ohta, M., Hirashita, T., Masuda, T., Inomata, M., Kitano. S. Obesity Surgery, Vol.23(12), p1947-1956, Dec 2013.

63.

 Effects of Sleeve Gastrectomy on Lipid Metabolism in an Obese Diabetic Rat Model. Kawano, Y., Ohta, M., Hirashita, T., Masuda, T., Inomata, M., Kitano. S. Obesity Surgery, Vol.23(12), p1947-1956, Dec 2013.

64.

Effects of electrical microstimulation of peripheral sympathetic nervous fascicle on glucose uptake in rats. Sato D., Shinzawa G., Kusunoki M., Matsui T., Sasaki H., Feng Z., Nishina A., Nakamura T. Journal of Artificial Organs, Vol.16(3), p352-358, Sep 2013.

65.

Long-term effect of green tea extract during lactation on AMPK expression in rat offspring exposed to fetal malnutrition. S.Sato, Y.Mukai, M.Hamaya, Y.Sun, M.Kurasaki. Nutrition, Vol.29(9), p1152-1158, Sep 2013.

66.

Effects of Sleeve Gastrectomy on Lipid Metabolism in an Obese Diabetic Rat Model. Y.Kawano, M.Ohta, T.Hirashita, T.Masuda, M.Inomata, S.Kitano. Obesity Surgery, Jul 2013.

67.

Glucose Use in Fasted Rats Under Sevoflurane Anesthesia and Propofol Anesthesia. K.Sato, T.Kitamura, G.Kawamura, Y.Mori, R.Sato, Y.Araki, Y.Yamada. Anesth Analg, Jun 2013.

68.

Oral administration of corn Zein hydrolysate stimulates GLP-1 and GIP secretion and improves glucose tolerance in male normal rats and Goto-Kakizaki rats. N.Higuchi, T.Hira, N.Yamada and H.Hara. Endocrinology, Jun 2013.

69.

Pancreatic stellate cells reduce insulin expression and induce apoptosis in pancreatic β-cells. K.Kikuta, A.Masamune, S.Hamada, T.Takikawa, E.Nakano, T.Shimosegawa. Biochemical and Biophysical Research Communications, Vol.433(3), p292-297, Apr 2013.

70.

Pancreatic stellate cells reduce insulin expression and induce apoptosis in pancreatic β-cells. Kikuta K, Masamune A, Hamada S, Takikawa T, Nakano E, Shimosegawa T. Biochemical and Biophysical Research Communications,Available online 13, Mar 2013

71.

Hypothalamic Brain-Derived Neurotrophic Factor Regulates Glucagon Secretion Mediated by Pancreatic Efferent Nerves. Gotoh K, Masaki T, Chiba S, Ando H, Fujiwara K, Shimasaki T, Mitsutomi K, Katsuragi I, Kakuma T, Sakata T, Yoshimatsu H. Journal of Neuroendocrinology, Vol.25(3), p302-311, Mar 2013.

72.

Effects of electrical microstimulation of peripheral sympathetic nervous fascicle on glucose uptake in rats. Sato D, Shinzawa G, Kusunoki M, Matsui T, Sasaki H, Feng Z, Nishina A, Nakamura T. Journal of Artificial Organs, Mar 2013.

73.

Improvement of erectile function by Korean red ginseng (Panax ginseng) in a male rat model of metabolic syndrome. Kim S-D, Kim Y-J, Huh J-S, Kim S-W and Sohn D-W. Asian Journal of Andrology , Feb 2013.

74.

Quercetin intake during lactation modulates the AMP-activated protein kinase pathway in the livers of adult male rat offspring programmed by maternal protein restriction. Sato S., Mukai Y., Saito T. The Journal of Nutritional Biochemistry, Vol.24(1), p118-123, Jan 2013.

75.

Reduction of reactive oxygen species ameliorates metabolism-secretion coupling in islets of diabetic GK rats by suppressing lactate overproduction. Sasaki M, Fujimoto S, Sato Y, Nishi Y, Mukai E, Yamano G, Sato H, Tahara Y, Ogura K, Nagashima K and Inagaki N. Diabetes, January 24, 2013 , In press.

76.

Derangement of ghrelin secretion after long-term high-fat diet feeding in rats. Sugiishi A, Kimura M, Kamiya R, Ueki S, Yoneya M, Saito Y, Saito H. Hepatology Research, 2013, In press.

77.

Proteomic and bioinformatic analysis of membrane proteome in type 2 diabetic mouse liver. Kim G-H, Park E C, Yun S-H, Hong Y, Lee D-G, Shin E-Y, Jung J, Kim Y H, Lee K-B, Jang I-S, Lee Z-W, Chung Y-H, Choi J-S, Cheong C, Kim S, Kim S II. PROTEOMICS, 2013, In press.

78.

A Novel Rat Model of Type 2 Diabetes: The Zucker Fatty Diabetes Mellitus ZFDM Rat. Yokoi N, Hoshino M, Hidaka S, Yoshida E, Beppu M, Hoshikawa R, Sudo K, Kawada A, Takagi S and Seino S. Journal of Diabetes Research, Vol.2013 (2013)

79.

Urinary cystatin C as a biomarker for diabetic nephropathy and its immunohistochemical localization in kidney in Zucker diabetic fatty (ZDF) rats. Togashi Y, Miyamoto Y. Experimental and Toxicologic Pathology,Available online 12 Jul 2012.

80.

Artemisia campestris leaf extract alleviates early diabetic nephropathy in rats by inhibiting protein oxidation and nitric oxide end products. Mediha S, Hamadi F, Nejla S, Yassine C, Mohamed M, Najiba Z. Pathology – Research and Practice, Vol.208(3), p157-162, Mar 2012.

81.

Fenugreek with reduced bitterness prevents diet-induced metabolic disorders in rats. Muraki E, Chiba H, Taketani K, Hoshino S, Tsuge N, Tsunoda N and Kasono K. Lipids in Health and Disease, Vol.11(58), 2012.

82.

Oral Ingestion of Aloe vera Phytosterols Alters Hepatic Gene Expression Profiles and Ameliorates Obesity-Associated Metabolic Disorders in Zucker Diabetic Fatty Rats. E. Misawa., M. Tanaka., K. Nomaguchi., K. Nabeshima., M. Yamada., T. Toida., and K. Iwatsuki. J. Agric. Food Chem., 2012, 60 (11), pp 2799-2806

83.

 Myocardial Infarction-Prone Watanabe Heritable Hyperlipidemic Rabbits with Mesenteric Fat Accumulation Are a Novel Animal Model for Metabolic Syndrome. M. Shiomi., T. Kobayashi., N. Kuniyoshi., S. Yamada., T. Ito. Pathobiology 2012;Vol. 79 No. 6 P329-338

84.

High-fat diet-induced reduction of peroxisome proliferator-activated receptor-γ coactivator-1α messenger RNA levels and oxidative capacity in the soleus muscle of rats with metabolic syndrome. F. Nagatomo., H. Fujino., H. Kondo., I. Takeda., K. Tsuda., A. Ishihara. Nutrition Research, Vol. 32, Issue 2, February 2012, Pages 144-151

85.

The effects of running exercise on oxidative capacity and PGC-1α mRNA levels in the soleus muscle of rats with metabolic syndrome. F. Nagatomo., H. Fujino., H. Kondo., M. Kouzaki., N. Gu., I. Takeda., K. Tsuda., and A. Ishihara. The Journal of Physiological Sciences, Vol. 62, Number 2 (2012), 105-114

86.

A Comparative Study of Gastric Banding and Sleeve Gastrectomy in an Obese Diabetic Rat Model. T. Masuda., M. Ohta., T. Hirashita., Y. Kawano., H. Egucji., K. Yada., Y. Iwashita., S. Kitano. Obesity Surgery, Published online:27 August 2011

87.

Site dependency of fatty acid composition in adipose triacylglycerol in rats and its absence as a result of high-fat feeding. D. Sato., T. Nakamura., K. Tsutsumi., G. Shinzawa., T. Karimata., T. Okawa., Z. Fengc., and M. Kusunoki. Metabolism.Article in Press

88.

Dietary fructo-oligosaccharides improve insulin sensitivity along with the suppression of adipocytokine secretion from mesenteric fat cells in rats. A. Shinoki., and H. Hara. British Journal of Nutrition.Published online :02 June 2011.

89.

Food restriction improves glucose and lipid metabolism through Sirt1 expression: A study using a new rat model with obesity and severe hypertension. K. Takemori.,T. Kimura.,N. Shirasaka.,T. Inoue.,K. Masuno., and H. Ito. Life Sciences.Vol.88, Issues 25-26, 1088-1094. 2011

90.

Enhanced Urinary Bladder, Liver and Colon Carcinogenesis in Zucker Diabetic Fatty Rats in a Multiorgan Carcinogenesis Bioassay: Evidence for Mechanisms Involving Activation of PI3K Signaling and Impairment of P53 on Urinary Bladder Carcinogenesis. N. Ishii., M. Wei., A. Kakehashi., K. Doi., S. Yamano., M. Inaba., and H.Wanibuchi. Journal of Toxicologic Pathology .Vol. 24 (2011) , No. 1 pp.25

91.

Maternal low-protein diet suppresses vascular and renal endothelial nitric oxide synthase phosphorylation in rat offspring independent of a postnatal fructose diet. S. Sato.,Y. Mukai., and T. Norikura. Journal of Developmental Origins of Health and Disease (2011), 2: 168-175

92.

Combined Effects of Short-term Calorie Restriction and Exercise on Insulin Action in Normal Rats. H,Y,Jiang.,T,Koike.,P,Li.,Z,H,Wang.,Y,Kawata.,Y,Oshida. Horm Metab Res 2010; 42(13): 950-954

93.

Dietary Hesperidin Exerts Hypoglycemic and Hypolipidemic Effects in Streptozocin-Induce Marginal Type 1 Diabetic Rats. Akiyama,S., Katsumata,S., Suzuki,K., Ishimi,Y.,Wu,J., and Uehara,M.. J Clin Biochem Nutr.January;46(1):87-92.2010

94.

Hypoglycemic and Hypolipidemic Effects of Hesperidin and Cyclodextrin-Clathrated Hesperetin in Goto-Kakizaki Rats with Type 2 Diabetes. Akiyama,S., Katsumata,S., Suzuki,K., Nakayama,Y., Ishimi,Y. and Uehara,M. Bioscience,Biotechnology,and Biochemistry.Vol.73,No.12 pp.2779-2782(2009)

95.

Anti-Diabetic Effects of Pumpkin and Its Components,Trigonelline and Nicotinic Acid,on Goto-Kakizaki Rats. Yoshinari,O.,Sato,H.and Igarashi,K. Bioscience,Biotechnology,and Biochemistry.Vol.73,No5pp.1033-1041,2009

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Dietary Phosphatidylinositol Prevents the Development of Nonalcoholic Fatty Liver Disease in Zucker(fa/fa)Rats Shirouchi,B.,Nagao,K.,Inoue,N.,Furuya,K.,Koga,S.,Matsumoto,H. and Yanagita,T. J.Agric.Food Chem.56,2375-2379,2008

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Investigation of the anti-obesity action of licorice flavonoid oil in diet-induced obese rats. Kamisoyama,H.,Honda,K.,Tominaga,Y.,Yokota,S.,Hasegawa,S. Bioscience.Biotechnology and Biochemistry 72.(12)3225-3231,2008

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Erythrophagocytosis by Liver Macrophages(Kupffer Cells)Promotes Oxidative Stress, Inflammation,and Fibrosis in a Rabbit Model of Steatohepatitis. Otogawa,K.,Kinoshita,K.,Fujii,H.,Sakabe,M.,Shiga,R.,Nakatani,K.,Ikeda,K.,Nakajima,Y.,Ikura,Y.,Ueda,M., Arakawa,T.,Hato,F., and Kawada,N. American Journal of Pathology. 170:967-980, 2007

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Age- and sex-related diferences in spontaneous hemorrhage and fibrosis of the pancreatic islets in Sprague-Dawley rats. Imaoka, M., Satoh, H. and Furuhama, K. Toxicologic Pathology 35: 388-394, 2007

100.

Effect of 5-Campestenone (24-methylcholest-5-en-3-one) on Zucker Diabetic Fatty Rats as a Type 2 Diabetes Mellitus Model. R.Konno, Y.,Kaneko, K.,Suzuki, Y.,Matsui. Horm Metab Res ; 37(3): 79-83,2005

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Oxidized but not acetylated low-density lipoprotein reduces preproinsulin mRNA expression and secretion of insulin from HIT-T15 cells. Okajima,F.,Kurihara,M.,Ono,C.,Nakajima,Y.,Tanimura,K.,Sugihara,H.,Ttsuguchi,A.,Nakagawa,K., Miyazawa,T.,and Oikawa,S. Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids, Volume 1687, Issues 1-3, pp.173-180, 2005

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The role of calcium/calmodulin-dependent protein kinase cascade in glucose upregulation of insulin gene expression. Xiao Yu, Koji Murao, Yoshitaka Sayo, Hitomi Imachi, Wen M. Cao, Shouji Ohtsuka, Michio Niimi, Hiroshi Tokumitsu, Hiroyuki Inuzuka, Norman C.W. Wong, Ryoji Kobayashi, and Toshihiko Ishida. Diabetes, 53: 1475-1481, 2004

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PVA hydrogel sheet macroencapsulation for the bioartificial pancreas. Qi M, Gu Y, Sakata N, Kim D, Shirouzu Y, Yamamoto C, Hiura A, Sumi S, Inoue K. Biomaterials. 25: 5885-5892, 2004

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Alterations in vascular endothelial function in the aorta and mesenteric artery in type II diabetic rats. Takayuki Matsumoto, Kentaro Wakabayashi, Tsuneo Kobayashi, and Katsuo Kamata

Can. J. Physiol. Pharmacol. 82(3): 175-182, 2004

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Effect of eicosapentaenoic acid ethyl ester v. oleic acid-rich safflower oil on insulin resistance in type 2 diabetic model rats with hypertriacylglycerolaemia. Asako Minami, Noriko Ishimura, Sadaichi Sakamoto, Eiko Takishita, Kazuaki Mawatari, Kazuko Okada and Yutaka Nakaya. British J Nutrition 87, 157-162, 2002.

106.

Evaluation of Insulin Secretion of Isolated Rat Islets Cultured in Extracellular Matrix. Nagata N.; Gu Y.; Hori H.; Balamurugan A.N.; Touma M.; Kawakami Y.; Wang W.; Baba T.T.; Satake A.; Nozawa M.; Tabata Y.; Inoue K. Cell Transplantation, 10, 447-451, 2001

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Lactoferrin ameliorates corticosterone-related acute stress and hyperglycemia in rats. Maekawa Y, Sugiyama A, Takeuchi T. J Vet Med Sci. 2017 Feb 28;79(2):412-417.

组成部分

状态

容量

(A) 抗体固相化 96孔板

洗净后使用

96 wells（8×12）/1 块

(B) 胰岛素标准溶液（小鼠）（200 ng/mL）

稀释后使用

25 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D) 生物素结合抗胰岛素抗体

稀释后使用

10 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

20 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1 瓶

(H) 反应终止液（1M   H₂SO₄）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

样品

A

B

C

D

mean

0.882

1.15

3.67

5.25

SD

0.0245

0.0213

0.0649

0.0792

CV(%)

2.78

1.87

1.77

1.51

测量日/样品

E

F

G

第０天

5.253

1.224

0.513

第１天

5.322

1.312

0.523

第２天

5.365

1.269

0.512

第３天

5.362

1.281

0.535

mean

5.326

1.272

0.521

SD

0.0520

0.0366

0.0109

CV(%)

3.31

3.76

4.65

添加量

实测值

回收量

回收率(%)

0

1.350

–

–

0.25

1.593

0.243

97.2

0.50

1.841

0.491

98.2

0.75

2.065

0.715

95.3

1.00

2.299

0.949

94.9

添加量

实测值

回收量

回收率(%)

0

1.941

–

–

0.50

2.496

0.505

101

0.75

2.728

0.737

98.3

1.00

2.955

0.964

96.4

1.50

3.431

1.440

96.0

ELISA试剂盒选择指南①②

 1.

Endoplasmic Reticulum Stress in Mice Increases Hepatic Expression of Genes Carrying a Premature Termination Codon via a Nutritional Status-Independent GRP78-Dependent Mechanism.Harada N, Okuyama M, Yoshikatsu A, Yamamoto H, Ishiwata S, Hamada C, Hirose T, Shono M, Kuroda M, Tsutsumi R, Takeo J, Taketani Y, Nakaya Y, Sakaue H.J Cell Biochem. 2017 Nov;118(11):3810-3824.

 2.

Effects of β-Glucan Content and Pearling of Barley in Diet-Induced Obese MiceSeiichiro Aoe, Yasunori Ichinose, Noriko Kohyama, Kozo Komae, Asuka Takahashi, Toji Yoshioka, and Takashi Yanagisawa.Posted online on 27 Sep 2017. https://doi.org/10.1094/CCHEM-04-17-0083-R

 3.

10-Hydroxy-2-decenoic acid, a natural product, improves hyperglycemia and insulin resistance in obese/diabetic KK-Ay mice, but does not prevent obesity Risa WATADANI, Jun KOTOH, Daiki SASAKI, Azusa SOMEYA, Kozo MATSUMOTO, Akihiko MAEDA Journal of Veterinary Medical Science, Vol. 79 (2017) No. 9 Sept, p.1596-1602

 4.

S-Equol Activates cAMP Signaling at the Plasma Membrane of INS-1 Pancreatic β-Cells and Protects against Streptozotocin-Induced Hyperglycemia by Increasing β-Cell Function in Male Mice. Horiuchi H, Usami A, Shirai R, Harada N, Ikushiro S, Sakaki T, Nakano Y, Inui H, Yamaji R. J Nutr. 2017 Sep;147(9):1631-1639.

 5.

Pathological and gene expression analysis of a polygenic diabetes model, NONcNZO10/LtJ mice. Hirata T, Yoshitomi T, Inoue M, Iigo Y, Matsumoto K, Kubota K, Shinagawa A. Gene. 2017 Sep 20;629:52-58.

 6.

Suppression of GRK2 expression reduces endothelial dysfunction by restoring glucose homeostasis. Taguchi K, Hida M, Hasegawa M, Narimatsu H, Matsumoto T, Kobayashi T. Sci Rep. 2017 Aug 16;7(1):8436.

 7.

Gender difference in NASH susceptibility: Roles of hepatocyte Ikkβ and Sult1e1. Matsushita N, Hassanein MT, Martinez-Clemente M, Lazaro R, French SW, Xie W, Lai K, Karin M, Tsukamoto H. PLoS One. 2017 Aug 10;12(8):e0181052.

 8.

Intake of mulberry 1-deoxynojirimycin prevents colorectal cancer in mice. E S, Yamamoto K, Sakamoto Y, Mizowaki Y, Iwagaki Y, Kimura T, Nakagawa K, Miyazawa T, Tsuduki T. J Clin Biochem Nutr. 2017 Jul;61(1):47-52.

 9.

Sake lees extract improves hepatic lipid accumulation in high fat diet-fed mice. Kubo H, Hoshi M, Matsumoto T, Irie M, Oura S, Tsutsumi H, Hata Y, Yamamoto Y, Saito K Lipids Health Dis. 2017 Jun 3;16(1):106.

10.

Moringa oleifera from Cambodia Ameliorates Oxidative Stress, Hyperglycemia, and Kidney Dysfunction in Type 2 Diabetic Mice. Tang Y, Choi EJ, Han WC, Oh M, Kim J, Hwang JY, Park PJ, Moon SH, Kim YS, Kim EK. J Med Food. 2017 May;20(5):502-510.

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4μ8C Inhibits Insulin Secretion Independent of IRE1α RNase Activity. Sato H, Shiba Y, Tsuchiya Y, Saito M, Kohno K. Cell Struct Funct. 2017 May 3;42(1):61-70

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Inhibition of Gastric Inhibitory Polypeptide Receptor Signaling in Adipose Tissue Reduces Insulin Resistance and Hepatic Steatosis in High-Fat Diet-Fed Mice. Joo E, Harada N, Yamane S, Fukushima T, Taura D, Iwasaki K, Sankoda A, Shibue K, Harada T, Suzuki K, Hamasaki A, Inagaki N. Diabetes. 2017 Apr;66(4):868-879.

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Royal jelly improves hyperglycemia in obese/diabetic KK-Ay mice. Mei YOSHIDA, Kaori HAYASHI,  Risa WATADANI,  Yoshiyasu OKANO, Keiya TANIMURA, Jun KOTOH, Daiki SASAKI,  Kozo MATSUMOTO,  Akihiko MAEDA Journal of Veterinary Medical Science, Vol. 79 (2017)  No. 2 February p. 299-307

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PDK1-FoxO1 pathway in AgRP neurons of arcuate nucleus promotes bone formation via GHRH-GH-IGF1 axis. Sasanuma H, Nakata M, Parmila K, Nakae J, Yada T. Mol Metab. 2017 Feb 17;6(5):428-439.

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Soy Protein Isolate Suppresses Lipodystrophy-induced Hepatic Lipid Accumulation in Model Mice. Nagao K, Matsumoto A, Kai S, Kayashima T, Yanagita T. J Oleo Sci. 2017 Feb 1;66(2):161-169.

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Dietary Mung Bean Protein Reduces Hepatic Steatosis, Fibrosis, and Inflammation in Male Mice with Diet-Induced, Nonalcoholic Fatty Liver Disease. Watanabe H, Inaba Y, Kimura K, Asahara SI, Kido Y, Matsumoto M, Motoyama T, Tachibana N, Kaneko S, Kohno M, Inoue H. J Nutr. 2017 Jan;147(1):52-60.

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Agmatine ameliorates type 2 diabetes induced-Alzheimer’s disease-like alterations in high-fat diet-fed mice via reactivation of blunted insulin signalling. Kang S, Kim CH, Jung H, Kim E, Song HT, Lee JE. Neuropharmacology. 2017 Feb;113(Pt A):467-479

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Dietary nitrite reverses features of postmenopausal metabolic syndrome induced by high fat diet and ovariectomy in mice. Ohtake K, Ehara N, Chiba H, Nakano G, Sonoda K, Ito J, Uchida H, Kobayashi J. Am J Physiol Endocrinol Metab. 2017 Feb 14

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Attenuated secretion of glucose-dependent insulinotropic polypeptide (GIP) does not alleviate hyperphagic obesity and insulin resistance in ob/ob mice. Satoko Shimazu-Kuwahara,Norio Harada,Shunsuke Yamane,Erina Joo,Akiko Sankoda,Timothy J. Kieffer,Nobuya Inagaki. Molecular Metabolism,Available online 19 January 2017

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Sodium alginate prevents progression of non-alcoholic steatohepatitis and liver carcinogenesis in obese and diabetic mice. Miyazaki T, Shirakami Y, Kubota M, Ideta T, Kochi T, Sakai H, Tanaka T, Moriwaki H, Shimizu M. Oncotarget. 2016 Mar 1;7(9):10448-58.

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Fatty Liver and Insulin Resistance in the Liver-Specific Knockout Mice of Mitogen Inducible Gene-6. Park BK, Lee EA, Kim HY, Lee JC, Kim KS, Jeong WH, Kim KY, Ku BJ, Rhee SD. J Diabetes Res. 2016;2016:1632061

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Reactive sulfur species regulate tRNA methylthiolation and contribute to insulin secretion. Takahashi N, Wei FY, Watanabe S, Hirayama M, Ohuchi Y, Fujimura A, Kaitsuka T, Ishii I, Sawa T, Nakayama H, Akaike T, Tomizawa K.

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Effects of caloric restriction on O-GlcNAcylation, Ca(2+) signaling, and learning impairment in the hippocampus of ob/ob mice. Jeon BT, Heo RW, Jeong EA, Yi CO, Lee JY, Kim KE, Kim H, Roh GS. Neurobiol Aging. 2016 Aug;44:127-37.

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Chronic Repression of mTOR Complex 2 Induces Changes in the Gut Microbiota of Diet-induced Obese Mice. Jung MJ, Lee J, Shin NR, Kim MS, Hyun DW, Yun JH, Kim PS, Whon TW, Bae JW. Sci Rep. 2016 Jul 29;6:30887.

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Single ingestion of soy β-conglycinin induces increased postprandial circulating FGF21 levels exerting beneficial health effects. Hashidume T, Kato A, Tanaka T, Miyoshi S, Itoh N, Nakata R, Inoue H, Oikawa A, Nakai Y, Shimizu M, Inoue J, Sato R. Sci Rep. 2016 Jun 17;6:28183.

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Pharmacological Inhibition of Monoacylglycerol O-Acyltransferase 2 Improves Hyperlipidemia, Obesity, and Diabetes by Change in Intestinal Fat Utilization. Take K, Mochida T, Maki T, Satomi Y, Hirayama M, Nakakariya M, Amano N, Adachi R, Sato K, Kitazaki T, Takekawa S. PLoS One. 2016 Mar 3;11(3):e0150976.

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Alteration of gut microbiota by vancomycin and bacitracin improves insulin resistance via glucagon-like peptide 1 in diet-induced obesity. Hwang I, Park YJ, Kim YR, Kim YN, Ka S, Lee HY, Seong JK, Seok YJ, Kim JB. FASEB J. Vol.29(6), p2397-411, Jun 2015.

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Insulin Release from the Beta Cells in Acatalasemic Mice Is Highly Susceptible to Alloxan-Induced Oxidative Stress. Kazunori Takemoto, Wakana Doi, Ken Kataoka, Kohji Ishihara, Da-Hong Wang., Hitoshi Sugiyama, Noriyoshi Masuoka. JDM, Vol.5 No.2, May 2015

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Essential role of mitochondrial Ca2+ uniporter in the generation of mitochondrial pH gradient and metabolism-secretion coupling in insulin-releasing cells. Quan X, Nguyen TT, Choi SK, Xu S, Das R, Cha SK, Kim N, Han J, Wiederkehr A, Wollheim CB, Park KS. J Biol Chem. Vol.290(7), p4086-96, Feb 2015.

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Endogenous Interleukin 18 Suppresses Hyperglycemia and Hyperinsulinemia during the Acute Phase of Endotoxemia in Mice. Yamashita H, Aoyama-Ishikawa M, Takahara M, Yamauchi C, Inoue T, Miyoshi M, Maeshige N, Usami M, Nakao A, Kotani J. Surg Infect (Larchmt). 2015 Feb;16(1):90-6.

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Alteration of gut microbiota by vancomycin and bacitracin improves insulin resistance via glucagon-like peptide 1 in diet-induced obesity. Hwang I, Park YJ, Kim YR1, Kim YN, Ka S, Lee HY, Seong JK, Seok YJ, Kim JB. FASEB J. Feb 2015.

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Chronic exposure to valproic acid promotes insulin release, reduces KATP channel current and does not affect Ca2+ signaling in mouse islets. Manaka K., Nakata M., Shimomura K., Rita RS., Maejima Y., Yoshida M., Dezaki K., Kakei M., Yada T. The Journal of Physiological Sciences, Vol.64(1), p77-83, Jan 2014.

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Azilsartan, an angiotensin II type 1 receptor blocker, restores endothelial function by reducing vascular inflammation and by increasing the phosphorylation ratio Ser1177/Thr497 of endothelial nitric oxide synthase in diabetic mice. Matsumoto S., Shimabukuro M., Fukuda D., Soeki T., Yamakawa K., Masuzaki H. and Sata M. Cardiovascular Diabetology, 13:30, 2014.

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Chronic treatment with novel GPR40 agonists improve whole-body glucose metabolism based on the glucose-dependent insulin secretion. H.Tanaka, S.Yoshida, H.Oshima, H.Minoura, K.Negoro, T.Yamazaki, S.Sakuda, F.Iwasaki, T.Matsui and M. Shibasaki. JPET, Jul 2013.

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Contribution of insulin signaling to the regulation of pancreatic beta-cell mass during the catch-up growth period in a low birth weight mouse model. Y.Yoshida, M.Fuchita, M.Kimura-Koyanagi, A.Kanno, T.Matsuda, S.Asahara, N.Hashimoto, T.Isagawa, W.Ogawa, H.Aburatani, T.Noda, S.Seino, M.Kasuga, Y.Kido. Diabetology International, Jul 2013.

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Ras-related C3 botulinum toxin substrate 1 (RAC1) regulates glucose-stimulated insulin secretion via modulation of F-actin. S.Asahara, Y.Shibutani, K.Teruyama, H.Y.Inoue, Y.Kawada, H.Etoh, T.Matsuda, M.Kimura-Koyanagi, N.Hashimoto, M.Sakahara, W.Fujimoto, H.Takahashi, S.Ueda, T.Hosooka, T.Satoh, H.Inoue, M.Matsumoto, A.Aiba, M.Kasuga, Y.Kido. Diabetologia, Vol.56(5), p1088-1097, May 2013.

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Anti-diabetic effect of amorphastilbol through PPARα/γ dual activation in db/db mice. Lee W, Ham J, Kwon H C, Kim Y K, Kim S-N. Biochemical and Biophysical Research Communications, Jan 2013.

101.

Transcriptional Regulatory Factor X6 (Rfx6) Increases Gastric Inhibitory Polypeptide (GIP) Expression in Enteroendocrine K-cells and Is Involved in GIP Hypersecretion in High Fat Diet-induced Obesity. Suzuki K, Harada N, Yamane S, Nakamura Y, Sasaki K, Nasteska D, Joo E, Shibue K, Harada T, Hamasaki A,Toyoda K, Nagashima K and Inagaki N. The Journal of Biological Chemistry, Vol.288, p1929-1938, Jan 2013.

102.

Apelin inhibits diet-induced obesity by enhancing lymphatic and blood vessel integrity. Sawane M, Kajiya K, Kidoya H, Takagi M, Muramatsu F and Takakura N. Diabetes, 2013.

103.

Beneficial effects of Allium sativum L. stem extract on lipid metabolism and antioxidant status in obese mice fed a high fat diet. Kim I, Kim H-R, Kim J-H, Om A-S. Journal of the Science of Food and Agriculture, 2013.

104.

Intake of mulberry 1-deoxynojirimycin prevents diet-induced obesity through increases in adiponectin in mice. Tsuduki T, Kikuchi I, Kimura T, Nakagawa K, Miyazawa T. Food Chemistry, Vol.139(14), p16-23, 2013.

105.

Effect of Mukitake mushroom (Panellus serotinus) on the pathogenesis of lipid abnormalities in obese, diabetic ob/ob mice. Inoue N, Inafuku M, Shirouchi B, Nagao K and Yanagita T. Lipids in Health and Disease, Vol.12(18), 2013.

106.

Pax6 Directly Down-Regulates Pcsk1n Expression Thereby Regulating PC1/3 Dependent Proinsulin Processing. Liu T., Zhao Y., Tang N., Feng R., Yang X., Lu N., Wen J., Li L. PLOS ONE, Vol.7(10), Oct 2012.

107.

Anti-Diabetic Atherosclerosis Effect of Prunella vulgaris in db/db Mice with Type 2 Diabetes. Hwang S M, Kim J K, Lee Y J, Yoon J J, Lee S M, Kang D G, Lee H S. Am J Chin Med, Vol.40, 2012.

108.

Prevention mechanisms of glucose intolerance and obesity by cacao liquor procyanidin extract in high-fat diet-fed C57BL/6 mice. Y. Yamashita., M. Okabe., M. Natsume., H. Ashida. Archives of Biochemistry and Biophysics, Available online 23 March 2012, In Press

109.

The Action of D-Dopachrome Tautomerase as an Adipokine in Adipocyte Lipid Metabolism. T. Iwata., H. Taniguchi., M. Kuwajima., T. Taniguchi., Y. Okuda., A. Sukeno., K. Ishimoto., N. Mizusawa., K. Yoshimoto. PLos ONE, Vol. 7(3), Mar 2012.

110.

Soymorphin-5, a soy-derived μ-opioid peptide, decreases glucose and triglyceride levels through activating adiponectin and PPARα systems in diabetic KKAy mice. Y. Yamada.,.A. Muraki.,.M. Oie.,.N. Kanegawa.,.A. Oda., Y. Sawashi., K. Kaneko., M. Yoshikawa., T. Goto., N. Takahashi., T. Kawada., and K. Ohinata. American Physiological Society, Vol.302, No.4, E433-E440, 2012.

111.

Effects of Gametophytes of Ecklonia Kurome on the Levels of Glucose and Triacylglycerol in db/db, Prediabetic C57BL/6J and IFN-γ KO Mice. F. Dwiranti., M. Hiraoka., T. Taguchi., Y. Konishi., M. Tominaga., A. Tominaga. Int J B 64 iomed Sci, Vol.8, No.1, Mar 2012.

112.

Endoplasmic Reticulum Stress Inhibits STAT3-Dependent Suppression of Hepatic Gluconeogenesis via Dephosphprylation and Deacetylation. K. Kimura., T. Yamada., M. Matsumoto., Y. Kido., T. Hosooka., S. Asahara., T. Matsuda., T. Ota., H. Watanabe., Y. Sai., K. Miyamoto., S. Kaneko., M. Kasuga., H. Inoue. Diabetes, Vol.61, No.1, p61-73, 2012.

113.

Melatonin protects mice with intermittent hypoxia from oxidative stress-induced pancreatic injury. LI G., HOU G., LU W., KANG J. Sleep and Biological Rhythms, Vol.9(2), p78-85, Apr 2011.

114.

A novel dipeptidyl peptidase IV inhibitor DA-1229 ameliorates streptozotocin-induced diabetes by increasing β-cell replication and neogenesis. J, M, Cho., H, W, Jang., H, Cheon.,Y, T, Jeong., D-H, Kim., Y-M, Lim., S, Choi., E, Yang., C-Y, Shin., M, H, Son., S, H, Kim., H, Kim., M, Lee. Diabetes Research and Clinical Practice.Vol. 91(1), p72-79, 2011.

组成部分

状态

容量

(A) 抗体固相化 96 孔板

洗净后使用

96 wells（8×12）/1 块

(B) 胰岛素标准溶液（小鼠）（200 ng/mL）

稀释后使用

300 μL/1 瓶

(C) 缓冲液（蓝色）

即用

60 mL/1 瓶

(D) 生物素结合抗胰岛素抗体

稀释后使用

200 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

200 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1 瓶

(H) 反应终止液（1M H₂SO₄）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

样品

A

B

C

1

0.749

10.6

81.9

2

0.749

10.7

80.9

3

0.749

10.6

82.3

4

0.711

10.8

82.9

5

0.711

10.8

81.9

mean

0.734

10.7

82.0

SD

0.021

0.11

0.72

CV(%)

2.8

1.0

0.88

测量日/样品

F

G

H

第0天

5.61

37.3

78.9

第1天

5.85

37.9

77.9

第2天

5.65

37.6

78.2

mean

5.70

37.6

78.3

SD

0.126

0.287

0.544

CV(%)

2.2

0.8

0.7

添加量

实测值

回收量

回收率(%)

0

1.10

–

–

1.0

2.16

1.06

106

2.5

3.60

2.50

100

5.1

6.06

4.96

97.3

添加量

实测值

回收量

回收率(%)

0

31.5

–

–

20

51.5

20.0

100

40

73.1

41.6

104

60

95.1

63.6

106

ELISA试剂盒选择指南①②

1.

Does a Treadmill Running Exercise Contribute to Preventing Deterioration of Bone Mineral Density and Bone Quality of the Femur in KK-Ay Mice, a Type 2 Diabetic Animal Model? Takagi S, Yamashita T, Miura T. Calcif Tissue Int. 2017 Aug 4. 

2.

Further characterization of diabetes mellitus and body weight loss in males of the congenic mouse strain DDD. Cg-Ay. Suto J, Satou K. The Journal of Veterinary Medical Science, 2015.

3.

Fat and carbohydrate in western diet contribute differently to hepatic lipid accumulation. Wu W, Tsuchida H, Kato T, Niwa H, Horikawa Y, Takeda J, Iizuka K. Biochem Biophys Res Commun. Vol.461(4), p681-6, Jun 2015.

4.

C-C Chemokine Receptor 2 Inhibitor Ameliorates Hepatic Steatosis by Improving ER Stress and Inflammation in a Type 2 Diabetic Mouse Model. Kim HM, Lee ES, Lee BR, Yadav D, Kim YM, Ko HJ, Park KS, Lee EY, Chung CH. PLoS One. 2015 Mar 27;10(3):e0120711.

5.

Viability and functional assessment of murine pancreatic islets after transportation between Korea and Japan. Lee S, Takahashi Y, Lee KM, Mizuno M, Nemeno JG, Takebe T, Lee JI. Transplant Proc. Vol.47(3), p738-41, Apr 2015.

6.

Calorie restriction-mediated restoration of hypothalamic signal transducer and activator of transcription 3 (STAT3) phosphorylation is not effective for lowering the body weight set point in IRS-2 knockout obese mice. Satoko Senda,  Atsushi Inoue,  Arshad Mahmood,  Ryo Suzuki,  Nozomu Kamei,  Naoto Kubota,  Taku Watanabe,  Masashi Aoyama,  Allah Nawaz,  Yoshiaki Ohkuma,  Koichi Tsuneyama,  Yukiko Koshimizu,  Isao Usui,  Kumiko Saeki,  Takashi Kadowaki,  Kazuyuki Tobe. Diabetology International February 2015

7.

Overweight in Mice and Enhanced Adipogenesis In Vitro are Associated with Lack of the Hedgehog Coreceptor Boc. Lee HJ, Jo SB, Romer AI, Lim HJ, Kim MJ, Koo SH, Krauss RS, Kang JS. Diabetes. 2015 Jan 9.

8.

Investigating the suspension culture on aggregation and function of mouse pancreatic β-cellsK.-C.Yang, C.-C.Wu, S.-H.Yang, C.-C.Chiu, S.Sumi, H.-S. Lee. Journal of Biomedical Materials Research Part A, Vol.101A(8), p2273-2282, Aug 2013.

9.

Microenvironment-regulated gene expression, morphology, and in vivo performance of mouse pancreatic β-cells. Chen P-Y., Wu C-C., Lu D-H., Sumi S., Lin F-H., Yange K-C. Process Biochemistry, Vol.48(1), p58-67, Jan 2013.

10.

The anti-ulcer agent, irsogladine, increases insulin secretion by MIN6 cells. T.Matsumoto，K.Sakurai, A.Tanaka, T.Ishibashi, K.Tachibana, K.Ishikawa, K.Yokote. European Journal of Pharmacology, Vol.685(1-3), p213-217, Jun 2012.

11.

Cell coupling regulates Ins1, Pdx-1 and MafA to promote insulin secretion in mouse pancreatic beta cells. K.-C. Yanga., Z. Qi., G. Yanai., Y. Shirouza., D.-H. Lu., H.-S. Lee., S. Sumi. Process Biochemistry, Vol.46(9), p1853-1860, 2011.

12.

Dietary Combination of Fish Oil and Taurine Decreases Fat Accumulation and Ameliorates Blood Glucose Levels in Type 2 Diabetic/Obese KK-Ay Mice. N. Mikami., M. Hosokawa., K. Miyashita. Journal of Food Science, Vol. 77(6), pH114-H120, Jun 2012.

13.

Cell coupling regulates Ins1, Pdx-1 and MafA to promote insulin secretion in mouse pancreatic beta cells. K.C. Yang., Z. Qi., G. Yanai., Y. Shirouza., D.H. Lu., H.S. Lee., and S. Sumi. Process Biochemistry.

组成

状态

容量

(A)   抗体固相化 96 孔板

洗净后使用

96 wells（8×12）/1 块

(B) 胰岛素标准液（猪）（240 ng/mL）

稀释后使用

25 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D) 生物素结合抗胰岛素抗体

稀释后使用

10 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

20 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1瓶

(H) 反应终止液（1M H₂SO₄）※小心轻放

即用

12 mL/1瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1瓶

封板膜

3 张

使用说明书

1 份

样品

A

B

C

mean

0.991

0.482

0.201

SD

0.0321

0.0175

0.0099

CV(%)

3.2

3.6

4.9

测量日/样品

D

E

F

mean

1.452

0.901

0.346

SD

0.0562

0.0321

0.0162

CV(%)

3.9

3.6

4.7

添加量

实测值

回收量

回收率(%)

–

0.343

–

–

0.25

0.576

0.233

93.2

0.5

0.802

0.459

91.7

1

1.295

0.952

95.2

2

2.301

1.958

97.9

添加量

实测值

回收量

回收率(%)

–

0.202

–

–

0.25

0.437

0.235

93.8

0.5

0.666

0.464

92.8

1

1.201

0.999

99.9

2

2.240

2.038

102

ELISA试剂盒选择指南①②