ShellDish Cell Culture Dish ShellDish Cell Culture Dish（含胶原涂层）品牌：Sanplatec

发表于2024年3月19日由上海金畔生物科技有限公司

ShellDish Cell Culture Dish

ShellDish Cell Culture Dish（含胶原涂层）

品牌：Sanplatec
CAS No.：
储存条件：室温
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
WEB26003	–	1个/包	–	咨询	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Micro Culture Capsule 0.2 um HT Tuffryn 品牌：Pall

发表于2024年2月23日由上海金畔生物科技有限公司

Micro Culture Capsule 0.2 um HT Tuffryn

品牌：Pall
CAS No.：
储存条件：室温
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
367-03181	–	1 pcs	–	咨询	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

Maxi Culture Capsule 0.2 um HT Tuffryn 品牌：Pall

发表于2024年2月20日由上海金畔生物科技有限公司

Maxi Culture Capsule 0.2 um HT Tuffryn

品牌：Pall
CAS No.：
储存条件：室温
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
360-03171	–	1 pcs	–	咨询	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

Culture Bag A-350NL 品牌：Funakoshi

发表于2024年1月29日由上海金畔生物科技有限公司

Culture Bag A-350NL

品牌：Funakoshi
CAS No.：
储存条件：参考产品说明书
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
87-352	–	5 sets	–	咨询	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

Culture Bag A-1000NL 品牌：Funakoshi

发表于2024年1月29日由上海金畔生物科技有限公司

Culture Bag A-1000NL

品牌：Funakoshi
CAS No.：
储存条件：参考产品说明书
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
87-302	–	5 sets	–	咨询	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

Connecting tube for culture bag 品牌：Funakoshi

发表于2024年1月28日由上海金畔生物科技有限公司

Connecting tube for culture bag

品牌：Funakoshi
CAS No.：
储存条件：参考产品说明书
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
87-360	–	50 pieces	–	咨询	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

96孔菌液培养板 Culture Plates

发表于2024年1月25日由上海金畔生物科技有限公司

96孔菌液培养板

Culture Plates

详细描述：

货号	产品名称	品牌	购买

货号	名称	单位	购买
740488.24	96孔菌液培养板	24 set	咨询客服
740488	96孔菌液培养板	4 set	咨询客服

96孔菌液培养板 Culture Plates

发表于2024年1月23日由上海金畔生物科技有限公司

96孔菌液培养板

Culture Plates

详细描述：

货号	产品名称	品牌	购买

货号	名称	单位	购买
740488.24	96孔菌液培养板	24 set	咨询客服
740488	96孔菌液培养板	4 set	咨询客服

Petri Dish for bacteria culture, 95mmφ 有盖培养皿（用于细菌培养）950mm 品牌：Sumitomo Bakelite

发表于2023年12月4日由上海金畔生物科技有限公司

Petri Dish for bacteria culture, 95mmφ

有盖培养皿（用于细菌培养）950mm

品牌：Sumitomo Bakelite
CAS No.：
储存条件：室温
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
MC-30950Z	–	500个	–		–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

Human Stem Cell Culture System (DEF-CS, all-in-one format)酶试剂盒Takara

发表于2023年11月27日由上海金畔生物科技有限公司

上海金畔生物科技有限公司代理Takara酶试剂盒全线产品，欢迎访问官网了解更多产品信息。

Human Stem Cell Culture System (DEF-CS, all-in-one format)
品牌	Code No.	产品名称	包装量	价格（元）	说明书	数量
Cellartis	Y30010	Cellartis^® DEF-CS^™ 500 Culture System	1 Kit	￥5,979

收藏产品加入购物车

无标题文档

Human iPS和Human ES增殖用培养基
(DEF-CS Culture System, all-in-one format)

作为一款高端创新型的产品，Cellartis DEF-CS培养系统使得单细胞干细胞操作成为常规的操作。该产品是专门针对人类诱导干细胞（hiPS）和人类胚胎干细胞（hES）研发的高效率增殖用培养基产品体系。该产品系统为all-in-one型，包括了所有组分，客户不需要另外求购组分。该产品是成分确定的培养基产品，而且不需要饲养层。DEF-CS既可以实现单细胞培养，也可以用于传统的iPS培养模式以及大规模干细胞增殖。在使用DEF-CS培养基增殖干细胞时，几乎没有背景分化的问题，这使得细胞筛选不再必需。作为一款创新产品，利用DEF-CS培养干细胞时，可以使用酶消化法（enzymatic passaging）实现需要单细胞操作，这一特点十分有利于高通量细胞鉴别筛选（high-throughput screening）、转染（transfection）、框架接种（scaffold seeding）等。

■ 产品特点

· Feeder-free and chemically defined culture system for efficient expansion of human iPS cells
· Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal and differentiation
· Complete kit includes 500 ml basal medium, additives, and coating compound
· Robust system provides high reproducibility and stable growth
· Maintains cells in an undifferentiated state with virtually no background differentiation

■ 产品应用

· Scale-up and mass production of human iPS cells
· Single-cell culture of human iPS cells
· Transfection and reprogramming
· High-throughput screening
· Tissue engineering (seeding cells on a scaffold)

■ 产品详情请点击：

Human Stem Cell Culture System (DEF-CS, all-in-one format)

Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The number of iPS cells was quantified after being cultured for three weeks using either the Cellartis DEF-CS Culture System, a reference feeder system, or four other stem cell culture systems.

参考文献：

1. Sivertsson, Louise, et al. "Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor." Stem cells and development 22.4 (2012): 581-594.
2. Hanson, Charles, et al. "Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro." Acta ophthalmologica 91.2 (2013): 127-130.
3. Norrman, Karin, et al. "Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level." Methods 59.1 (2013): 59-70.
4. Ulvestad, Maria, et al. "Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells." Biochemical pharmacology 86.5 (2013): 691-702.
5. Ramirez JM, et al. Side scatter intensity is highly heterogeneous in undifferentiated pluripotent stem cells and predicts clonogenic self-renewal. Stem Cells Dev.2013 Jun 15;22(12):1851-1860.
6. Borestrom, Cecilia, et al. “Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: A first step toward a clinical-grade cell source.” Stem Cells Trans. Med. (2014) 3, 433-447.
7. Kia, Richard, et al. "MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity." Toxicological Sciences (2014): kfu269.
8. Valton, Julien, et al. "Efficient strategies for TALEN-mediated genome editing in mammalian cell lines." Methods 69.2 (2014): 151-170.
9. Zandén, Carl, et al. "Stem cell responses to plasma surface modified electrospun polyurethane scaffolds." Nanomedicine: Nanotechnology, Biology and Medicine 10.5 (2014): 949-958.
10. Asplund, Annika, et al. “One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying? Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells” Stem Cell Rev and Rep (2015)

页面更新：2020-02-14 15:52:16

Human Stem Cell Culture Medium (DEF-CS Xeno-Free)酶试剂盒Takara

发表于2023年11月25日由上海金畔生物科技有限公司

上海金畔生物科技有限公司代理Takara酶试剂盒全线产品，欢迎访问官网了解更多产品信息。

Human Stem Cell Culture Medium (DEF-CS Xeno-Free)
品牌	Code No.	产品名称	包装量	价格（元）	说明书	数量
Cellartis	Y30045	Cellartis^® DEF-CS^™ 500 Xeno-Free Culture Medium w/o antibiotics	1 Kit	￥2,923
Cellartis	Y30047	Cellartis^® DEF-CS^™ 500 Xeno-Free 3D Spheroid Culture Medium	1 Kit	￥3,747
Cellartis	Y30071	Cellartis^® DEF-CS^™ 500 Xeno-Free GMP Grade Basal Medium (Prototype)	500 ml	￥6,088

收藏产品加入购物车

无标题文档

临床前扩增人干细胞培养基系列（Xeno-free)
(The Xeno-Free Culture Media Product Family for Preclinical Expansion of Human iPS Cells)

Cellartis DEF-CS (Xeno-free) 培养基系列，完全无任何人源及动物源成分，被用于人类诱导多能干细胞 (iPSCs) 扩增。该款产品不使用饲养层 (feeder)，而是使用xeno-free的基质物，比如新型的iMatrix-511。使用DEF-CS 培养基 (xeno-free) 培养的细胞，拥有高的增殖率和稳定的核型，允许2D-单层培养和3D-悬浮培养进行有效扩增。

这些产品主要适用于：

· 基础研究和临床前概念研究—Cellartis DEF-CS 500 Xeno-Free Culture Medium帮助您从基础研究过渡到下一阶段相关疾病模型的验证和生产制造战略的开展。

· 扩增到足够的细胞数—Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium搭配灌注生物反应器系统，提供了一个产生大量高质量的、未分化的、用作起始细胞治疗材料的理想方法。

· GMP 级生产和下游使用于临床研究—Cellartis DEF-CS 500 Xeno-Free GMP Grade Basal Medium (Prototype)遵循严格的生产准则，确保没有可能损害患者的人类和动物来源的物质。

■ 产品特点

Cellartis DEF-CS 500 Xeno-Free Culture Medium
· Maintains pluripotency and stable karyotype in a 2D non-colony monolayer culture
· Ideal for maintenance of undifferentiated pluripotent cells and scale-up to therapeutically relevant cell numbers
· Fully compatible with the GMP-grade version for a seamless transition to the clinic
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium
· Pluripotent cells in the spheroids retain full differentiation potential and stable karyotype
· Produces 5 × 10⁹ cells in a 1 L bioreactor within 3–4 passages
· Provides a distinct advantage for scale-up to therapeutically relevant cell numbers
Cellartis DEF-CS 500 Xeno-Free GMP Grade Basal Medium (Prototype)
· Optimized for small- or large-scale production of pluripotent cells for downstream use in clinical research settings
· Manufactured as a quality-assured product, according to the guidelines for GMP for investigational products
· Rigorous manufacturing standards ensure quality and consistency; all materials are of clinical quality, with traceable production processes

■ 产品应用

· Culturing human iPS cells in completely xeno-free conditions for preclinical studies.

■ 产品目录

Code No.	Product	Size
Y30045	Cellartis^® DEF-CS^™ 500 Xeno-Free Culture Medium w/o antibiotics	1 kit
Y30042	Cellartis^® DEF-CS^™ 500 Xeno-Free Additives	1 kit
Y30046	Cellartis^® DEF-CS^™ 500 Xeno-Free Basal Medium w/o antibiotics	500 ml

Code No.	Product	Size
Y30047	Cellartis^® DEF-CS^™500 Xeno-Free 3D Spheroid Culture Medium w/o antibiotics	1 kit
Y30046	Cellartis^® DEF-CS^™ 500 Xeno-Free Basal Medium w/o antibiotics	500 ml
Y30048	Cellartis^® DEF-CS^™500 Xeno-Free 3D Spheroid Additives	1 kit

Code No.	Product	Size
Y30071	Cellartis^® DEF-CS^™ 500 Xeno-Free GMP Grade Basal Medium (Prototype)	500 ml

■ 关联产品

Code No.	Product	Size
T303	iMatrix-511	2 × 175 μg

■ 产品详情请点击

Y30045：
Y30047：
Y30071：

Human Stem Cell Culture Medium (DEF-CS Xeno-Free)

High-quality hiPS cell aggregates grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). hiPS cell aggregates grown under 4% O2 perfusion conditions were sequentially passaged three times by mechanical disruption (protocol adapted from Otsuji et al. 2014); cells were counted at passage on days 4 and 7, and at the final time point on day 11, to determine the expansion factor relative to the initial seeding number.

High-quality hiPS cell aggregates were grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). After expansion of hiPS cells as aggregates, cells were harvested on day 4 and expression of OCT4, SSEA-4, TRA-1-60 (pluripotency markers) and SSEA-1 and SOX17 (differentiation markers) was analyzed by immunostaining.

Takara Bio USA, Inc.比较结果

页面更新：2019-11-25 15:36:15