特点

本试剂盒用于监测系统热应力条件下的蛋白稳定性。

品质保证

每批次 PROTEOSTAT^®Thermal shift 稳定性检测试剂盒的样品试剂盒均已按照操作手册中的步骤进行测试。

16 μg/ml β-乳球蛋白的聚集温度单峰位于76°±2°C处。

使用/稳定性

在产品标签注明的日期前，试剂盒组分在适当的储存条件下可稳定保存。

可存放于-20°C的无霜冰箱中。

储存条件

避光。避免反复冻融。

运输方式

干冰运输

储存温度

-20°C

组分

· PROTEOSTAT^® TS 检测试剂

· β-乳球蛋白对照

· 10X 检测缓冲液

技术信息/产品说明

应用说明：

Prediction of Aggregation Propensity   and Monitoring of Aggregation of Antibody-Drug Conjugates (ADC) using   ProteoStat^® Reagents

检测样本：

PROTEOSTAT^® Cited Samples

Enzo及PROTEOSTAT均为Enzo Life Sciences, Inc. 的商标。Grenier为Grenier Bio-One 的注册商标。Enzo的部分产品和产品应用受美国、外国专利以及正在申请的专利的保护。本产品由ENZO LIFE SCIENCES, INC. 制造和销售，仅供研究市场的终端用户作研究使用，不得用于诊断或治疗用途。购买行为不包括在商业上使用、开发或以其他方式利用本产品的任何权利或许可。未经ENZO LIFE SCIENCES, INC. 事先明确书面授权，严禁对本产品进行任何商业用途、开发或利用，或使用本产品进行研发。

监管状态

RUO – 仅供研究使用

产品编号

产品名称

包装

ENZ-51027-K400

PROTEOSTAT^® Thermal shift stability assay kit
PROTEOSTAT^® 热稳定检测试剂盒

400 tests

ENZ-51027-K100

100 tests

1.

Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system: S. Murakami, et al.; Sci. Rep. 9, 671 (2019), Application(s): Purified IgG or Trastuzumab, 摘要; 全文

2.

Structural insights into humanization of anti-tissue factor antibody 10H10: A. Teplyakov, et al.; MAbs. 10, 269 (2018), Application(s): Monoclonal antibodies, 摘要; 全文

3.

Protein catalyzed capture agents with tailored performance for in vitro and in vivo applications: M.B. Coppock, et al.; Biopolymers 108, e22934 (2017), Application(s): Stability of peptide-based ligands, 摘要;

4.

Time-Dependent Protein Thermostability Assay: I. Vandecaetsbeek, et al.; Methods Mol. Biol. 1377, 79 (2016), 摘要;

5.

Effect of Polyethylene Glycol Conjugation on Conformational and Colloidal Stability of a Monoclonal Antibody Antigen-Binding Fragment (Fab'): C. Roque, et al.; Mol. Pharm. 12, 562 (2015), 摘要;

6.

Modulating the thermostability of Endoglucanase I from Trichoderma reesei using computational approaches: G. Bayram Akcapinar, et al.; Protein Eng. Des. Sel. 28, 127 (2015), 摘要;

7.

Site-specific conjugation of monomethyl auristatin E to anti-CD30 antibodies improves their pharmacokinetics and therapeutic index in rodent models: F. Lhospice, et al.; Mol. Pharm. 12, 1863 (2015), 摘要;

8.

A general approach to antibody thermostabilization: A.D. McConnell, et al.; MAbs 6, 1274 (2014), Application(s): Stability of antibodies, 摘要; 全文

9.

Modifications of cysteine residues in the transmembrane and cytoplasmic domains of a recombinant hemagglutinin protein prevents cross-linked multimer formation and potency loss: K.M. Holtz, et al.; BMC Biotechnol. 14, 111 (2014), 摘要;

10.

An integrated approach to extreme thermostabilization and affinity maturation of an antibody: A.D. McConnell, et al.; Protein Eng. Des. Sel. 26, 151 (2013), 摘要;

11.

Mouse, but not human STING, binds and signals in response to the vascular disrupting agent 5,6-dimethylxanthenone-4-acetic acid: J. Conlon, et al.; J. Immunol. 190, 5216 (2013), 摘要;

12.

Optimization of protein purification and characterization using Thermofluor screens: S. Boivin, et al.; Protein Expr. Purif. 91, 192 (2013), (Review), 摘要;

13.

Protein quality control acts on folding intermediates to shape the effects of mutations on organismal fitness: S. Bershtein, et al.; Mol. Cell 49, 133 (2013), Application(s): Aggregation propensity of the DHFR proteins, 摘要;

其他参考文献

1.

Estimation of protein aggregation propensity with a melting point apparatus: A.A. Raibekas; Anal. Biochem. 380, 331 (2008), 摘要;

2.

Thermofluor-based high-throughput stability optimization of proteins for structural studies: U.B. Ericsson, et al.; Anal. Biochem. 357, 289 (2006), 摘要;

3.

Structure of the cross-beta spine of amyloid-like fibrils: R. Nelson, et al.; Nature 435, 773 (2005), 摘要;

4.

Study of the thermal denaturation of ribonuclease A by differential thermal analysis and susceptibility to proteolysis: B.G. Winchester, et al.; Biochem J. 117, 299 (1970), 摘要;