详细描述: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MC
详细描述: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MC
Nagasawa T, Inada Y, Nakano S, Tamura T, Takahashi T, Maruyama K, Yamazaki Y, Kuroda J, Shibata N. Effects of bezafibrate, PPAR pan-agonist, and GW501516, PPARdelta agonist, on development of steatohepatitis in mice fed a methionine- and choline-deficient diet. Eur J Pharmacol 2006;536:182-91
Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) HRP substrate for low-picogram-level detection by Western blot analysis.
SuperSignal West Pico Substrate provides intense signal and picogram sensitivity for Western blotting with horseradish peroxidase (HRP) enzyme conjugates. The ECL substrate is compatible with different membranes, blocking reagents and a wide range of antibody dilutions. SuperSignal West Pico Substrate provides excellent performance, versatility and economy for routine Western blotting needs.
Features of SuperSignal West Pico Chemiluminescent Substrate:
• ECL—enhanced chemiluminescent substrate for horseradish peroxidase (HRP) • Picogram sensitivity—detect low-picogram amounts of protein in bands on nitrocellulose or PVDF membrane • Good signal duration—incubated blots provide 6 to 8 hours of usable light output when conditions are optimized • Stable reagent—24-hour working solution stability; 1-year kit stability at room temperature • Economical—optimized for dilute antibody concentrations: • 0.2 to 1.0 µg/mL primary antibody (1:1000 to 1:5000 dilution from 1 mg/mL stock) • 10 to 50 ng/mL secondary antibody (1:20,000 to 1:100,000 dilution from 1 mg/mL stock)
THERMO/pierce化学发光底物(皮克级)34077 34080
内容及储存
Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane • Luminol/Enhancer, 50 mL • Stable Peroxide Buffer, 50 mL
Gibco™ KnockOut™ Serum Replacement (KnockOut™ SR) is a more defined, FBS-free medium supplement that supports the growth of pluripotent stem cells (PSCs) cultured on fibroblast feeder cells. Designed to replace fetal bovine serum (FBS) in existing protocols, this serum replacement has been trusted by many for almost 20 years. Providing the documents required for regulatory review, KnockOut SR reduces your burden in qualifying reagents when transitioning from research applications to clinical applications. If you are interested in only basic research applications, see the Research Use Only version of this product to ensure that you receive the benefits and pricing that fit your needs.
With KnockOut Serum Replacement, you can: • Culture feeder-dependent PSCs with confidence • Rely on the improved performance of a qualified, FBS-free formulation • Enjoy the peace of mind of having a more stable price and supply compared to FBS
Culture PSCs with confidence KnockOut SR has been cited in more than 2000 publications. It has been shown to support feeder-dependent culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). It has also been shown to support reprogramming, embryoid body (EB) formation, in vitro differentiation, cryopreservation, ESC derivation, and many other applications.
qiagen石蜡组织DNA提取试剂盒56404,QIAamp DNA FFPE Tissue Kit
QIAamp DNA FFPE Tissue Kit专为从福尔马林固定、石蜡包埋组织中纯化DNA而设计。该试剂盒应用特殊的裂解方式脱离组织切片中的DNA,克服了福尔马林交联造成的抑制效应。试剂盒通过QIAamp MinElute离心柱纯化高品质DNA,洗脱体积小。QIAamp DNA FFPE Tissue Kit可在QIAcube全自动核酸纯化仪上自动纯化DNA。
qiagen石蜡组织DNA提取试剂盒56404,QIAamp DNA FFPE Tissue Kit
Principle
QIAamp DNA FFPE Tissue Kit使用成熟的QIAamp MinElute技术,从小体积样本中纯化基因组DNA和线粒体DNA。该试剂盒同时具备硅胶膜选择性结合DNA的特性,以及20 µl到100 µl的灵活洗脱体积。
Intended use For In Vitro diagnostic use. This product is suitable for mounting tissue specimens, cell smears, and cytospins which have been stained with fluorochromes such as fluorescein in preparation for viewing by fluorescence microscopy. Refer to the “General Instructions for Immunohistochemical Staining” or the Detection System “Instructions” of IHC procedures for: (1) Principle of Procedure, (2) Materials Required, Not Supplied, (3) Storage, (4) Specimen Preparation, (5) Staining Procedure, (6) Quality Control, (7) Troubleshooting, (8) Interpretation of Staining, (9) General Limitations. Reagent provided 15 mL of fluorescent mounting medium containing an anti-fading agent and 0.015 mol/L sodium azide. Precautions 1. For professional users. 2. Wear appropriate personal protective equipment to avoid eye and skin contact. 3. Unused solution should be disposed of according to local, State and Federal regulations Storage Store at 2–8°C. Do not freeze.
DAKO Fluorescence Mounting Medium货号S3023
Application Fluorescent Mounting Medium enhances the visualization of specimens when viewed under a fluorescent microscope. The unique formulation of this product retards the fading of fluorescence allowing the specimens to be reviewed long after mounting. Slides that are mounted with Fluorescent Mounting Medium and stored at 2–8°C in the dark preserve thei r fluorescent signal for at least one month. Fluorescent Mounting Medium dries compley when slides are coverslipped forming a solid coating that facilitates handling and storage. Procedure 1. Dispense one or two drops of Fluorescent Mounting Medium onto tissue sections or cell preparations which are mounted on a glass microscope slide. 2. Apply glass coverslip over Fluorescent Mounting Medium and allow the medium to spread evenly over the specimen. 3. Slides may be examined immediay under a fluorescent microscope. However, the coverslips should not be moved as mounting medium will not compley solidify for several minutes. 4. For long-term storage the edges of the coverslip may be sealed with nail polish to prevent air pockets from forming.
详细描述: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MC
详细资料: 产品简单说明:Isopore 表面滤膜,聚碳酸酯,亲水,0.22 µm,142 mm,白色,光面 商标名:Isopore 产品包装:50/PK 滤膜材质:聚碳酸酯(Polycarbonate) 滤膜商标名:Isopore 折射率:1.6 23 °C 时的泡点:≥5.5 bar, air with water zui高操作温度:140°C 滤膜类型:表面滤膜 滤膜孔径:0.22um 可润湿性:亲水 滤膜直径:142mm 滤膜代码:GTTP 滤膜颜色:白色 产品名称:Isopore 表面滤膜 滤膜表面:光面 重量分析溶出物:<1% 厚度:25–30um 孔隙率:13.8%
应用
应用
This 142 mm Isopore hydrophilic membrane with 0.2 µm pore size is a polycarbonate, track-etched screen filter recommended for analyses of airborne contaminants & other particles using optical or electron microscopy.