详细描述： The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded
PD SpinTrap G-25 is a single-use column for cleanup of large biomolecules
Convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr) with the possibility to run multiple samples in parallel.
Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
High desalting capacity.
Reproducible, high throughput, parallel cleanup of protein samples.
Sample preparation in the range of 100 to 180 μl without sample dilution.
PD SpinTrap G-25 is a single-use microspin column that is designed for desalting and buffer exchange of biological sample using a standard microcentrifuge. The column is prepacked with ~600 μl Sephadex G-25 Medium, and the column material is biocompatible polypropylene.
Instructions supplied with the columns
详细参数：
PD SpinTrap G-25
Certificate of Analysis
Yes
Sample Volume
100-180 μl
Complete Packsize
50 Columns
Storage Conditions
4 to 30°C, 0,05% Kathon CG/ICP Biocide
Pressure Max. [Over the Packed Bed During Operation]
N/A (N/A)
Wetted Part
No
Media
BioProcess resin
Yes
Matrix
Cross-linked dextran
pH Stability Working Range
2–13
pH Stability Cleaning
2–13
pH stability, CIP
2–13
Pressure/Flow Specification
>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.
Exclusion Limit [Mr] [Globular Proteins]
> 5 x 103
Exclusion Limit
Mr 5000
Chemical Stability
All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
Nitrocellulose blotting membrane, with 0.45 µm pore size, provides excellent sensitivity, resolution, and low background in fluorescence Western blotting detection applications.
High binding capacity (162 – 180 µg IgG/cm2).
Suitable for proteins of a wide range of molecular weights.
Unsupported, 100% pure nitrocellulose membrane for use with all ECL Western blotting systems and protein blotting applications.
Available in popular precut sizes for your application.
Amersham Protran Premium offers excellent sensitivity, resolution, and low background, especially when used with the fluorescence Amersham ECL Plex Western Blotting Detection System.
For convenience, Western blotting sandwiches (10 sandwiches/package) are available, consisting of precut Amersham Protran Premium 0.45 NC membranes preassembled with 2 x 3MM Chr Blotting papers.
详细描述： The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded
Nitrocellulose membrane, with a 0.45 µm pore size, is suitable for proteins of a wide range of molecular weights.
Low background and excellent signal-to-noise ratios.
Compatible with a variety of labeling and detection systems.
High binding capacity.
Suitable for proteins of a wide range of molecular weights.
Proprietary nitrocellulose formula gives a long shelf life of bound proteins.
Available in popular precut sizes for your application.
Amersham Protran offers excellent sensitivity, resolution, and low background, especially when used with Amersham ECL, Amersham ECL Prime, or Amersham ECL Select Western Blotting Systems.
For convenience, Western blotting sandwiches (10 sandwiches/package) are available, consisting of precut Amersham Protran 0.45 NC membranes preassembled with 2 x 3MM Chr Blotting papers.
Nitrocellulose membrane, with a 0.2 µm pore size and high surface area, for binding of small proteins (< Mr 20 000).
Low background and excellent signal-to-noise ratios, without the need for stringent washing conditions. Compatible with a variety of labeling and detection systems.
High binding capacity of small proteins (< Mr 20 000) reduces risk of “blow-through”.
Proprietary nitrocellulose formula gives a long shelf life of bound proteins.
Available in popular precut sizes for your application.
Amersham Protran offers excellent sensitivity, resolution, and low background, especially when used with Amersham ECL, Amersham ECL Prime, or Amersham ECL Select Western Blotting Systems.
For convenience, Western blotting sandwiches (10 sandwiches/package) are available, consisting of precut Amersham Protran 0.20 NC membranes preassembled with 2 x 3MM Chr Blotting papers.
Thermo Scientific Pierce EDTA-Free Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments.
THERMO pierce蛋白酶抑制剂Protease Inhibitor Mini Tablets, EDTA Free
Features of EDTA-Free Protease Inhibitor Mini Tablets:
Features of EDTA-Free Protease Inhibitor Mini Tablets:
• Economical—More cost effective than other commercially available formulations for equivalent volumes • Easy-to-use—the refrigerator-stable tablets are easily dissolved in buffers and lysis reagents • EDTA-free—formulated without EDTA, a metalloproteinase inhibitor • Compatible—added compatibility with IMAC purification methods and 2D gel electrophoresis
Pierce Protease Inhibitor Tablets protect proteins from proteolytic degradation through inhibition of the serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A. Stable for storage at 4°C for up to 12 months, each mini tablet is sufficient for 10 mL of solution, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.
Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids. Proteases are regulated and compartmentalized in living cells. Protein extraction from tissues and cultured cells causes their release and ability to degrade other valuable proteins,
详细描述： The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded
Life Technologies 大家庭中的细胞培养产品。 Life Technologies公司已准备就绪，以满足您的细胞培养需要一个全面的选择，包括培养基，营养添加剂，盐溶液，缓冲液，化学物质，解离试剂和转染的试剂。您可以依靠严格的质量控制和，以保证您的满意。仅供研究使用。 不用于任何动物或人类治疗或诊断使用。
