LBIS® 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒 LBIS® GLP-1(active) ELISA KIT

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  • 相关资料
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  • 参考文献

LBIS® GLP-1(active) ELISA KITLBIS® 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒                              LBIS® GLP-1(active) ELISA KIT

LBIS® 胰高血糖素样肽-1(GLP-1)(活性) ELISA 试剂盒

LBIS® 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒                              LBIS® GLP-1(active) ELISA KIT


胰高血糖素样肽-1(Glucagon-like peptide-1,GLP-1)是胰高血糖素前驱体的一部分。胰高血糖素前驱体于胰脏、小肠下部以及下丘脑中表达。该前驱体的构造中含有与糖代谢有关的各种各样的生理活性物质(胰高血糖素,肠高血糖素,胃泌酸调节素,GLP-1,GLP-2)的氨基酸序列。根据表达部位加工酶的特异性,胰脏主要分泌胰高血糖素,而小肠下部主要分泌肠高血糖素和胃泌酸调节素。GLP-1 和 GLP-2 则存在于胰高血糖素前驱体后半的结构中。GLP-1由37个氨基酸组成,有2种生物活性形式,分别为 GLP-1(7-37)和GLP-1(7-36)酰胺。两者都存在于小肠下部、胰脏和下丘脑中,GLP-1(7-36)酰胺在下丘脑中占免疫反应 GLP-1(IR-GLP-1)总量的 55-94%,在小肠中占 27-73%。但在胰脏中只有极少量存在。大部分哺乳类(如人类、大鼠、小鼠、牛、猪、狗等)的 GLP-1 结构相似。

 

GLP-1: hdeferhaegtftsdvssylegqaakefiawlvkgrg  

GLP 1(7-37): haegtftsdvssylegqaakefiawlvkgrg

GLP 1(7-36) amide: haegtftsdvssylegqaakefiawlvkgr-NH2

 

GLP-1 与小肠上部分泌的 GIP 统称为肠促胰素。该类激素是葡萄糖浓度依赖性方式促进胰岛素分泌。同时具有抑制胃肠道蠕动和胃液分泌、抑制胰高血糖素的释放、促进生长抑素的分泌、使食欲减退,促进肠上皮细胞生长、以及外周组织促进非胰岛素依赖性的葡萄糖的消耗,并促进细胞的生长的作用。有报告指出该类激素与垂体激素的分泌也有关系。

GLP-1(7-36)酰胺在生物体内代谢迅速,DPP-IV(dipeptidyl peptidase IV)会使其失去N-末端的两个氨基酸变为 GLP-1(9-36)酰胺,GLP-1(7-37)变为 GLP-1(9-37)后会失去活性。有报告指出,体外实验中,在犬的血浆中 GLP-1(7-36)酰胺的半衰期是为 61±9 分,GLP-1(7-37)为 132±16 分。因此 GLP-1 的测定,取样的时候有必要使用 DPP-IV 抑制剂。

此外,肠促胰素中的 GIP 则是有力促进 GLP-1 分泌的激素。回肠中 GLP-1 的分泌不是食物直接刺激肠道而产生的,而是由于胆碱能和肽类的刺激所产生的。

◆特点

● 短时间测定(完全反应时间:5小时)

● 微量样品(标准操作用量:10 μL)可测

● 使用对环境无害的防腐剂

● 全部试剂均为液体,可直接使用

● 精密的测定精度和高再现性

◆构成

 

组成

状态

容量

(A) 抗体固相化 96 孔板

洗净后使用

96   wells(8×12)/1 块

(B) GLP-1标准溶液(500 pg/mL

稀释后使用

200 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D)生物素结合抗GLP-1抗体

稀释后使用

100 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

100 μL/1 瓶

(F) 显色液(TMB)

即用

12 mL/1 瓶

(H) 反应终止液(1M H2SO4)※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液(10×)

稀释后使用

100 mL/1 瓶

封板膜

4 张

使用说明书

1 份

◆交叉反应

※交差率是 1,000 pg/mL 浓度时的数据

动物类型

对象物质

反应性和反应率(%)

Mouse/Rat

GLP-1(7-36)amide

100

GLP-1(7-37)

<   0.1

GLP-1(1-37)

GLP-1(9-36)amide

GLP-2

Glucagon(1-29)

Insulin

Secretin

GIP

VIP

GRF

Bovine

Glucagon(1-29)

VIP

Porcine

Glucagon(1-29)

VIP

―:不存在交叉反应

◆样品信息

 

小鼠和大鼠的血清及血浆

10 μL/well(标准操作方法)

※测量中由于酶(DPP-IV 等)的影响,采血时请注意防止 GLP-1(7-36)酰胺的分解,再使用。

 

 

◆测定范围

 

1.56~50.0 pg/mL 【0.47~15.16 pmol/L(分子量3298)】(标准曲线范围)

7.8~250 pg/mL(样品量 10 μL)

3.9~125 pg/mL(样品量 20 μL)

◆Validation data

 

精度测试(检测内变异系数)

 

样品

A

B

1

23.7

6.44

2

23.2

5.97

3

23.4

6.39

4

24.0

5.87

5

24.1

6.44

mean

23.7

6.22

SD

0.35

0.28

CV(%)

1.5

4.5

单位:pg/mL

 

 

再现性测试(检测内变异系数)

 

测量日/样品

E

F

第0天

25.1

6.31

第1天

25.1

6.16

第2天

25.0

6.24

第3天

25.0

6.37

mean

25.0

6.27

SD

0.03

0.09

CV(%)

0.13

1.4

单位:pg/mL n=4

 

 

添加回收测试

 

样品C

添加量

实测值

回收量

回收率(%)

0.00

3.93

3.26

7.28

3.35

103

6.51

10.3

6.37

97.8

8.14

12.1

8.17

100

 

样品D

添加量

实测值

回收量

回收率(%)

0.00

11.8

7.16

19.1

7.30

102

14.3

25.5

13.7

95.8

21.5

32.4

20.6

95.8

 

 

稀释直线性测试

 

用稀释缓冲液分三次连续稀释2个血清样品的结果,直线回归方程的R2在0.997~0.9999之间。

相关资料


LBIS® 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒                              LBIS® GLP-1(active) ELISA KIT LBIS® 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒                              LBIS® GLP-1(active) ELISA KIT

ELISA试剂盒选择指南①②

ELISA试剂盒选择指③④

参考文献

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Intestinal Bile Acid Composition Modulates Prohormone Convertase 1/3 (PC1/3) Expression and Consequent GLP-1 Production in male mice. Morimoto K, Watanabe M, Sugizaki T, Irie JI, Itoh H. Endocrinology. 2016 Jan 20:en20151551.


16.

Hypoxia decreases glucagon-like peptide-1 secretion from the GLUTag cell line. Kihira Y, Burentogtokh A, Itoh M, Izawa-Ishizawa Y, Ishizawa K, Ikeda Y, Tsuchiya K, Tamaki T. Biol Pharm Bull. Vol.38(4), p514-21, 2015.


17.

Dietary obacunone supplementation stimulates muscle hypertrophy, and suppresses hyperglycemia and obesity through the TGR5 and PPARγ pathway. Horiba T, Katsukawa M, Mita M, Sato R. Biochem Biophys Res Commun. Vol.463(4), p846-52, Aug 2015.


18.

Combination of DPP-4 inhibitor and PPARγ agonist exerts protective effects on pancreatic β-cells in diabetic db/db mice through the augmentation of IRS-2 expression. Hirukawa H, Kaneto H, Shimoda M, Kimura T, Okauchi S, Obata A, Kohara K, Hamamoto S, Tawaramoto K, Hashiramoto M, Kaku K. Mol Cell Endocrinol. Jun 2015.


19.

Glucagon-like peptide-1 is specifically involved in sweet taste transmission. Takai S, Yasumatsu K, Inoue M, Iwata S, Yoshida R, Shigemura N, Yanagawa Y, Drucker DJ, Margolskee RF, Ninomiya Y. FASEB J. Vol.29(6), p2268-80, Jun 2015.


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22.

Duodenal-jejunal bypass improves diabetes and liver steatosis via enhanced glucagon-like peptide-1 elicited by bile acids. Kashihara H, Shimada M, Kurita N, Sato H, Yoshikawa K, Higashijima J, Chikakiyo M, Nishi M, Takasu C. J Gastroenterol Hepatol. Vol.30(2), p308-15, Feb 2015.


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26.

Oral administration of osteocalcin improves glucose utilization by stimulating glucagon-like peptide-1 secretion. Mizokami A, Yasutake Y, Higashi S, Kawakubo-Yasukochi T, Chishaki S, Takahashi I, Takeuchi H, Hirata M. Bone. 16;69C:68-79. Sep 2014.


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产品编号 产品名称 产品规格 产品等级
637-15129 (AKMGP-011) LBIS® GLP-1(Active) ELISA Kit
LBIS® 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒 
96 tests

胰高血糖素ELISA试剂盒 Glucagon-Like ELISA kit

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

胰高血糖素ELISA试剂盒胰高血糖素ELISA试剂盒                              Glucagon-Like ELISA kit


原理

  Glicentin:肠高血糖素(Glicentin)是69个氨基酸的多肽,包含胰高血糖素和胃泌酸调节素两部分序列。在哺乳动物的小肠中,胰高血糖素原经过加工后可形成肠高血糖素、胃泌酸调节素和胰高血糖素样肽-1(GLP-1)、胰高血糖素样肽-2(GLP-2)。



优点、特色

●  简单快速

●  高特异性

●  超灵敏度


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产品编号 产品名称 产品规格 产品等级
YII-YK111-EX Glicentin, EIA
 肠高血糖素ELISA试剂盒
1 kit
YII-YK140-EX Glucagon-Like Peptide 2 EIA
 大鼠胰高血糖素样肽2(GLP-2)ELISA试剂盒
1 kit
YII-YK141-EX Glucagon-Like Peptide 2 EIA
 人胰高血糖素样肽2(GLP-2)ELISA试剂盒
1 kit
YII-YK142-EX Glucagon-Like Peptide 2 EIA
 小鼠胰高血糖素样肽2(GLP-2)ELISA试剂盒
1 kit
YII-YK160-EX GlucagonLike Peptide 1 EIA KIT
 胰高血糖素样肽1(GLP-1)ELISA试剂盒
1 kit