详细描述： In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about
从凋亡到坏死的过渡是一个松散定义的连续体。质膜细胞外表面上磷脂酰丝氨酸（PS）的暴露仍是早期细胞凋亡的共同标志。这时候在Ca2+存在下，磷脂结合蛋白例如 Annexin V 与 PS 具有亲和力。由于 Annexin V 不具有细胞渗透性，所以这种胞外PS的结合是早期凋亡细胞对具有选择性的。
同样，质膜完整性的丧失，如不具有膜渗透性DNA嵌入染料若能标记出细胞核，则是表明晚期细胞凋亡和坏死的直接方法。考虑到Annexin V通常与荧光素（又称FITC）偶联，检测绿色荧光蛋白（GFP）表达细胞中的凋亡时可能会出现问题。以上两个荧光团的发射图谱会发生光谱重叠，使信号之间的区分变得困难或不可能，从而损害了总体上的数据质量。GFP-CERTIFIED® 细胞凋亡/坏死检测系统专为表达GFP的细胞系以及表达蓝色或青色荧光蛋白（BFP、CFPs）的细胞而设计，同时也可以应用于不带荧光蛋白的细胞的凋亡和坏死的细胞。另外，本试剂盒适合用于活细胞或结合了探针（如标记抗体、或与荧光素或香豆素显示相似光谱特性的其他荧光偶联物）的细胞。本试剂盒包含了用于确定细胞凋亡及坏死的早期和晚期阶段中所需的全部试剂。Annexin V -EnzoGold（增强型Cyanine-3）偶联物能够检测不同于荧光素或GFP的细胞凋亡。坏死检测试剂（红色）类似于发出红色的染料7-AAD，可促进晚期细胞凋亡和坏死检测。本试剂盒还包含了可用作阳性对照的凋亡诱导剂（Staurosporine）。检测试剂可单独使用或分别与其他单独或多重应用组合使用。本试剂盒还可以通过流式细胞仪和荧光/共焦显微镜提供快速、准确的检查结果。
应用说明： Image-Based Analysis of a Human Neurosphere Stem Cell Model for the Evaluation of Potential
Neurotoxicants 引用样本： For an overview on cited samples please clickhere.
监管状况：
RUO-仅供研究用
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PKM2 coordinates glycolysis with mitochondrial fusion and oxidative phosphorylation: T. Li, et al.; Protein Cell 10, 583 (2019), Application(s): Flow cytometry using H1299 and HepG2 cells, 摘要;
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Cluster microRNAs miR‐194 and miR‐215 suppress the tumorigenicity of intestinal tumor organoids: T. Nakaoka, et al.; Cancer Sci. 108, 678 (2017), Application(s): Flow cytometry using organoid culture of mouse intestinal tumors, 摘要; 全文
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Metabolic modulation of Ewing sarcoma cells inhibits tumor growth and stem cell properties: A. Dasgupta, et al.; Oncotarget. 8, 77292 (2017), Application(s): Flow cytometry with A673 cells, 摘要; 全文
17.
Multiple hyperthermia-mediated release of TRAIL/SPION nanocomplex from thermosensitive polymeric hydrogels for combination cancer therapy: Z.Q. Zhang, et al.; Biomaterials. 132, 16 (2017), Application(s): Confocal microscopy with PC3 and U-87 MG cells, 摘要;
18.
Pro-metastatic intracellular signaling of the elaidic trans fatty acid: K. Fujii, et al.; Int. J. Oncol. 50, 85 (2017), 摘要;
19.
Visualization of ceramide channels in lysosomes following endogenous palmitoyl-ceramide accumulation as an initial step in the induction of necrosis: M. Yamane, et al.; Biochem. Biophys. Rep. 11, 174 (2017), Application(s): Confocal microscopy with A549 cells, 摘要; 全文
20.
Immunotherapy of acute leukemia by chimeric antigen receptor-modified lymphocytes using an improved Sleeping Beauty transposon platform: C.F. Magnani, et al.; Oncotarget 7, 51581 (2016), Application(s): Flow cytometry using KG-1, REH, Nalm-6, and THP-1 cells, 摘要; 全文
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Novel long chain fatty acid derivatives of quercetin-3-O-glucoside reduce cytotoxicity induced by cigarette smoke toxicants in human fetal lung fibroblasts: N. Sumudu, et al.; Eur. J. Pharmacol. 781, 128 (2016),摘要;
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23.
PKLR promotes colorectal cancer liver colonization through induction of glutathione synthesis: A. Nguyen, et al.; J. Clin. Invest. 126, 681 (2016), Application(s): Assessed Apoptosis, Flow cytometry, 摘要; 全文
24.
Protective effects of β‐casofensin, a bioactive peptide from bovine β‐casein, against indomethacin‐induced intestinal lesions in rats: C. Bessette, et al.; Mol. Nutr. Food Res. 60, 823 (2016), 摘要;
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BRCA1, PARP1 and γH2AX in acute myeloid leukemia: Role as biomarkers of response to the PARP inhibitor olaparib: I. Faraoni, et al.; Biochim. Biophys. Acta 1852, 462 (2015), Application(s): Flow cytometry using primary AML cells, 摘要;
26.
Growth-promoting and tumourigenic activity of c-Myc is suppressed by Hhex: V. Marfil, et al.; Oncogene 34, 3011 (2015), Application(s): Flow cytometry using HO15.19 and TGR-1 cells, 摘要;
27.
common set of proteins modulated in Chikungunya virus infection: R. Abraham, et al.; J. Proteomics. 120, 126 (2015), Application(s): Flow cytometry using HEK293 cells, 摘要;
28.
Identification of novel osteogenic compounds by an ex-vivo sp7: luciferase zebrafish scale assay: E. de Vrieze, et al.; Blood 74, 106 (2015), Application(s): Fluorescence microscopy using fish scales, 摘要;
29.
Impaired oxidative phosphorylation regulates necroptosis in human lung epithelial cells: M.J. Koo, et al.; Biochem. Biophys. Res. Commun. 464, 875 (2015), Application(s): Apoptosis/necrosis assay, 摘要;
30.
Influenza virus M2 targets cystic fibrosis transmembrane conductance regulator for lysosomal degradation during viral infection: J.D. Londino, et al.; FASEB J. 29, 2712 (2015), Application(s): Flow cytometry using HEK293 cells, 摘要; 全文
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Inhibition of autophagy exerts anti-colon cancer effects via apoptosis induced by p53 activation and ER stress: K. Sakitani, et al.; BMC Cancer 15, 795 (2015), Application(s): Flow cytometric analysis of apoptosis, 摘要; 全文
32.
N-terminal functional domain of Gasdermin A3 regulates mitochondrial homeostasis via mitochondrial targeting: P.H. Lin, et al.; J. Biomed. Sci. 22, 44 (2015), Application(s): Fluorescence microscopy using HaCaT and HEK293 cells, 摘要; 全文
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NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival: J.M. Calandria, et al.; Cell Death Differ. 22, 1363 (2015), Application(s): Assay, 摘要;
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Apoptotic and inhibitory effects on cell proliferation of hepatocellular carcinoma HepG2 cells by methanol leaf extract of Costus speciosus: S.V. Nair, et al.; Biomed. Res. Int. 2014, Article ID 637098 (2014), Application(s): Measurement by flow cytometry, 摘要; 全文
40.
Combination of antithrombin and recombinant thrombomodulin modulates neutrophil cell-death and decreases circulating DAMPs levels in endotoxemic rats: T. Iba, et al.; Throm. Res. 134, 169 (2014), Application(s): Detection Kit using live cells, 摘要;
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Analysis of protein translocation into the endoplasmic reticulum of human cells: J. Dudek, et al.; Methods Mol. Biol. 1033, 285 (2013), 摘要;
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55.
Methionine excess in diet induces acute lethal hepatitis in mice lacking cystathionine γ-lyase, an animal model of cystathioninuria: H. Yamada, et al.; Free Radic. Biol. Med. 52, 1716 (2012), Application(s): Fluorescence microscopy using mouse hepatocytes, 摘要;
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58.
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Two mutations impair the stability and function of ubiquitin-activating enzyme (E1): T. Lao, et al.; J. Cell. Physiol. 227, 1561 (2012), Application(s): Viability and induction of cell death observed by confocal microscopy, 摘要; 全文
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Supplemental Information for: Cell Surface Externalization of Annexin A1 as a Failsafe Mechanism Preventing Inflammatory Responses during Secondary Necrosis: K.E. Blume, et al.; J. Immunol. 183, (2009), (Supplemental Information), 全文
NUCLEAR-ID® DNA 红色染料细胞渗透性 DNA 染色，可广泛应用于高效液相色谱（HPLC）≥93％，流式细胞仪，荧光检测
产品编号
包装
ENZ-51014-100
200 µL
参考文献
1.
Growth-promoting and tumorigenic activity of c-Myc is suppressed by Hhex: V. Marfil, et al.; Oncogene 34, 3011 (2015), Abstract;
2.
Epigenetic regulation of planarian stem cells by the SET1/MLL family of histone methyltransferases: A. Hubert, et al.; Epigenetics 8, 79 (2013), Application(s): Cell cycle analysis of stem cells from freshwater planarian by flow cytometry, Abstract; Full Text
3.
Gene-specific factors determine mitotic expression and bookmarking via alternate regulatory elements: P. Arampatzi, et al.; Nucleic Acids Res. 41, 2202 (2013), Application(s): Fluorescence imaging of nuclear DNA of human cancer cells, Abstract; Full Text
4.
An intact retinoblastoma protein-binding site in Merkel cell polyomavirus large T antigen is required for promoting growth of Merkel cell carcinoma cells: R. Houben, et al.; Int. J. Cancer 130, 847 (2012), Application(s): Cell cycle analysis of cancer cell lines by flow cytometry, Abstract; Full Text
5.
Gomisin A enhances tumor necrosis factor-α-induced G1 cell cycle arrest via signal transducer and activator of transcription 1-mediated phosphorylation of retinoblastoma protein: P. Waiwut, et al.; Biol. Pharm. Bull. 35, 1997 (2012), Application(s): Cell cycle analysis of HeLa cells by flow cytometry,Abstract; Full Text
6.
A cell-permanent dye for cell cycle analysis by flow and laser-scanning microplatecytometry: Y.J. Xiang, et al.; Nat. Methods 6, an2 (2009), Abstract;
一般参考文献
1.
DNA measurement and cell cycle analysis by flow cytometry: R. Nunez; Curr. Issues Mol. Biol. 3, 67 (2001), Abstract;
2.
Methods in Cell Biology, Flow Cytometry: Z. Darzynkiewicz, H.A. Crissman and J.P. Robinson (editors and co-authors); Vol. I and II, (1994), Book,
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ENZ-51014-100
Nuclear-ID® Green cell cycle kit for flow cytometry 细胞周期检测试剂盒（绿色荧光）（流式）
1 Kit
–
ENZ-51008-100
Nuclear-ID® Red cell cycle kit (GFP-Certified®) for flow cytometry 细胞核检测试剂盒（红色荧光）（GFP细胞）（荧光显微镜）
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