UBF (F-9) 抗体 (Alexa Fluor® 488 标记) UBF (F-9) Alexa Fluor® 488

UBF (F-9) 抗体 (Alexa Fluor® 488 标记)

UBF (F-9) Alexa Fluor® 488

详细描述:
The transcription of ribosomal RNA genes by RNA polymerase I (Pol I) is tightly coordinated with the growth state of the cell. In addition to Pol I, transcription of ribosomal genes requires the trans-activating factor UBF (upstream binding factor). UBF f

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:AF488 。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-13125 AF488 UBF (F-9) 抗体 (Alexa Fluor® 488 标记) 200 µg/ml 咨询客服

Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 (mammalian derived)

详细介绍

 

Recombinant Human TGF-β1 (mammalian derived)

Catalog Number:
100-21
Synonyms:
Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor
Description:
The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Human TGF-β1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond.
AA Sequence:
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Source:
HEK293 cells
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 was determined by TGF-beta1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
 
 
 
 
Cross Reactivity:
Chicken, Cow, Dog, Frog, Monkey, Mouse, Pig, Rabbit, Rat, Human, Mink, Donkey, N/A
Research Interests:
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 (mammalian derived) Angiogenesis/ Cardiovascular Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 (mammalian derived) Apoptosis
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 (mammalian derived) Cancer Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 (mammalian derived) Immune System
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 (mammalian derived) Inflammation Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 (mammalian derived) Proliferation
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 (mammalian derived) Stem Cells & Differentiation Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 (mammalian derived) TGF-β Superfamily
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 (mammalian derived) Wound Healing Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 (mammalian derived) Allergy
 
 
Country of Origin:
USA

Biolegend LEAF™ Purified anti-human CD3 Antibody

详细介绍

LEAF™ Purified anti-human CD3 Antibody

Product Details
 
 
  All CD3 Reagents
Clone: OKT3 (See other available formats)
Isotype: Mouse IgG2a, κ
Isotype Control: LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl
Reactivity: Human
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The CD3 antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:

FC – Quality tested
IHC, Activ – Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

COA:

Enter Lot#:     Biolegend LEAF&trade; Purified anti-human CD3 Antibody

Application
Notes:

Clone OKT3 can block the binding of clones SK7 and UCHT1.8 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application
References:

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
4. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
5. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
6. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
7. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
8. Li B, et al. 2005. Immunology 116:487.

  Publication Library
Biolegend LEAF&trade; Purified anti-human CD3 Antibody

Human peripheral blood lymphocytes stained with LEAF™ purified OKT3, followed by anti-mouse IgG FITC

Compare all formats 
 

 
 

Description:

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Other Names: T3, CD3ε
Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK-T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand Receptor: Peptide antigen bound to MHC
Antigen
References:

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 
3. Lanier L, et al. 1986. J. Immunol. 137:2501.

GeneID: 916 Biolegend LEAF&trade; Purified anti-human CD3 Antibody
Latest Publications: View the latest CD3 articles on HighwirePress.com
UniProt: View information about CD3 on UniProt.org
Keywords: LEAF™ Purified anti-human CD3, OKT3, LEAF™, T3, CD3ε
Related Products
 
Description Clone Applications
LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC, WB, IP, ICFC, ICC, IF, IHC, FA
Cell Staining Buffer   FC, ICC, ICFC
RBC Lysis Buffer (10X)   FC, ICFC

Other Formats

 
Description Clone Applications
Alexa Fluor® 488 anti-human CD3 OKT3 FC
Alexa Fluor® 647 anti-human CD3 OKT3 FC
APC anti-human CD3 OKT3 FC
Biotin anti-human CD3 OKT3 FC
Brilliant Violet 510™ anti-human CD3 OKT3 FC
Brilliant Violet 605™ anti-human CD3 OKT3 FC
Brilliant Violet 650™ anti-human CD3 OKT3 FC
Brilliant Violet 711™ anti-human CD3 OKT3 FC
Brilliant Violet 785™ anti-human CD3 OKT3 FC
FITC anti-human CD3 OKT3 FC
Pacific Blue™ anti-human CD3 OKT3 FC
PE anti-human CD3 OKT3 FC
PE/Cy7 anti-human CD3 OKT3 FC
PerCP/Cy5.5 anti-human CD3 OKT3 FC
Purified anti-human CD3 OKT3 FC, IHC
Ultra-LEAF™ Purified anti-human CD3 OKT3 FC, IHC, Activ

 

 

 

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上海金畔生物科技有限公司代理biolegend产品,咨询

 

PALL转印膜NC膜66485现货供应

详细介绍

PALL转印膜NC膜66485现货供应

纯自由NC膜非常适合菌落/菌斑提取和蛋白转印
1.坚固耐用。与同类NC膜相比更耐磨,更不容易破裂。
2.对蛋白质和核酸具有良好的结合能力。
3.比同等电泳传输设备的蛋白质溶穿率更低。
介质
NC

孔的尺寸
0.2µm
典型厚度
145µm(5.7mils)
蛋白质接合能力
209µg/cm2
1.坚固耐用。与同类NC膜相比更耐磨,更不容易破裂。
2.对蛋白质和核酸具有良好的结合能力。
3.比同等电泳传输设备的蛋白质溶穿率更低。

PALL转印膜NC膜66485现货供应订货信息:

Part Number Description Pkg
BioTrace NT Nitrocellulose Transfer Membrane
66487 82 mm discs 50/pkg
66595 85 mm discs (gamma irradiated) 50/pkg
66518 132 mm discs 50/pkg
66488 137 mm discs 50/pkg
66593 7 x 8.5 cm sheets 10/pkg
66489 20 x 20 cm sheets 10/pkg
66485 30 cm x 3 m roll 1/pkg
 

1.坚固耐用。与同类NC膜相比更耐磨,更不容易破裂。
2.对蛋白质和核酸具有良好的结合能力。
3.比同等电泳传输设备的蛋白质溶穿率更低。