荧光底物Mca-PLAQAV-Dpa-RSSSR-NH2

	货号	13560	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	1800
	Ex (nm)	322	Em (nm)	381
	分子量	1638.96	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：13560

产品名称：荧光底物Mca-PLAQAV-Dpa-RSSSR-NH2

规格：1mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：1638.96

溶剂：DMSO

激发波长(nm)：322

发射波长(nm)：381

校正系数（280nm）:0.3

产品介绍

Mca-PLAQAV-Dpa-RSSSR-NH2是用于检测肿瘤坏死因子-α转换酶（TACE或ADAM17）和相关酶（如ADAM8，ADAM9和ADAM10）的荧光肽底物。其N端包含高荧光的7-甲氧基香豆素（MCA）荧光团，可通过荧光共振能量转移（Dark FRET）通过Dpa上的非荧光2,4-二硝基苯基有效地淬灭。它可用于测量能够裂解荧光基团和淬灭基团之间酰胺键的肽酶活性，从而增加荧光强度。该肽序列衍生自促肿瘤坏死因子-α（TNF-α）。 TACE / ADAM17和ADAM10的切割位点是A（Ala）和V（Val）残基之间的肽键。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的荧光底物Mca-PLAQAV-Dpa-RSSSR-NH2。 

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参考文献

Increased plasma TACE activity in subjects with mild cognitive impairment and patients with Alzheimer’s disease.
Authors: Sun, Qiying and Hampel, Harald and Blennow, Kaj and Lista, Simone and Levey, Allan and Tang, Beisha and Li, Rena and Shen, Yong
Journal: Journal of Alzheimer’s disease : JAD (2014): 877-86

Primary structure and characterization of a non hemorrhagic metalloproteinase with fibrinolytic activity, from the snake venom of Protobothrops tokarensis (Tokara-habu).
Authors: Oyama, Etsuko and Kitagawa, Yasuyuki and Takahashi, Hidenobu
Journal: Toxicon : official journal of the International Society on Toxinology (2013): 153-61

ADAM-17: the enzyme that does it all.
Authors: Gooz, Monika
Journal: Critical reviews in biochemistry and molecular biology (2010): 146-69

Expression of pro-inflammatory TACE-TNF-α-amphiregulin axis in Sjögren’s syndrome salivary glands.
Authors: Sisto, Margherita and Lisi, Sabrina and Lofrumento, Dario Domenico and Ingravallo, Giuseppe and Mitolo, Vincenzo and D’Amore, Massimo
Journal: Histochemistry and cell biology (2010): 345-53

Constitutive endocytosis of the chemokine CX3CL1 prevents its degradation by cell surface metalloproteases.
Authors: Huang, Yi-Wei and Su, Paul and Liu, Guang Ying and Crow, Min Rui and Chaukos, Deanna and Yan, Harry and Robinson, Lisa A
Journal: The Journal of biological chemistry (2009): 29644-53

Drug insight: tumor necrosis factor-converting enzyme as a pharmaceutical target for rheumatoid arthritis.
Authors: Moss, Marcia L and Sklair-Tavron, Liora and Nudelman, Raphael
Journal: Nature clinical practice. Rheumatology (2008): 300-9

Expressions of tumor necrosis factor-converting enzyme and ErbB3 in rats with chronic obstructive pulmonary disease.
Authors: Ju, Chun-rong and Xia, Xi-zheng and Chen, Rong-chang
Journal: Chinese medical journal (2007): 1505-10

Photic injury promotes cleavage of p75NTR by TACE and nuclear trafficking of the p75 intracellular domain.
Authors: Srinivasan, Bhooma and Wang, Zhaohui and Brun-Zinkernagel, Anne M and Collier, Robert J and Black, Roy A and Frank, Stuart J and Barker, Philip A and Roque, Rouel S
Journal: Molecular and cellular neurosciences (2007): 449-61

Relationships between early inflammatory response to bleomycin and sensitivity to lung fibrosis: a role for dipeptidyl-peptidase I and tissue inhibitor of metalloproteinase-3?
Authors: Pottier, Nicolas and Chupin, Cécile and Defamie, Virginie and Cardinaud, Bruno and Sutherland, Rachel and Rios, Géraldine and Gauthier, Francis and Wolters, Paul J and Berthiaume, Yves and Barbry, Pascal and Mari, Bernard
Journal: American journal of respiratory and critical care medicine (2007): 1098-107

Characterization of Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2, a fluorogenic substrate with increased specificity constants for collagenases and tumor necrosis factor converting enzyme.
Authors: Neumann, Ulf and Kubota, Hisashi and Frei, Karl and Ganu, Vishwas and Leppert, David
Journal: Analytical biochemistry (2004): 166-73

7-Deaza-7-Propargylamino-3′-azidomethyl-dATP

	货号	17090	存储条件	在零下15度以下保存, 避免光照
	规格	50 nmoles	价格	4884
	Ex (nm)		Em (nm)
	分子量	598.30	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17090

产品名称：7-Deaza-7-Propargylamino-3′-azidomethyl-dATP

规格：50 nmoles

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：598.30

溶剂：水

产品介绍

7-Deaza-7-Propargylamino-3′-azidomethyl-dATP是制备用于下一代测序（NGS）的荧光偶联物的关键组成部分。 NGS使用与早期Sanger测序相似的链终止方法，但NGS是通过荧光标记的核苷酸类似物作为扩增反应的可逆终止剂来进行的。 NGS依赖于可逆的DNA聚合阻断，而Sanger测序则使用ddNTP不可逆转的DNA聚合阻断。 NGS的另一个不同特征是通过桥式PCR进行体外克隆扩增以增加待测序分子的数量。在此实验上，片段与固定在固体表面上的引物连接，进行原位扩增，生成具有相同分子的DNA簇。在每个循环中，同时添加可逆终止的四个核苷酸，并通过它们互补的聚合酶掺入。通过将3′-OH基团替换为3′-o-叠氮基甲基，这些核苷酸被化学封闭，以防止聚合酶在每个循环中掺入一个以上的核苷酸。掺入核苷酸后，在不同通道中针对不同碱基测量荧光信号。关于下一循环，洗涤未掺入的核苷酸，并用TCEP去除3’端的化学封锁。一旦收集到荧光信号，就会开始一个新的循环，重复此动态过程，直到完成每个片段的测序为止。总之，NGS测序反应分三个步骤进行：核苷酸的添加，成像和通过荧光团裂解的3′-OH再生。

参考文献

Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR.
Authors: Castellanos-Rizaldos, Elena and Richardson, Katherine and Lin, Rui and Wu, Grant and Makrigiorgos, Mike G
Journal: Clinical chemistry (2015): 267-77

Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: assembly, annotation and molecular marker discovery.
Authors: Ahn, Yul-Kyun and Tripathi, Swati and Kim, Jeong-Ho and Cho, Young-Il and Lee, Hye-Eun and Kim, Do-Sun and Woo, Jong-Gyu and Cho, Myeong-Cheoul
Journal: Gene (2014): 494-9

Mutant firefly luciferases with improved specific activity and dATP discrimination constructed by yeast cell surface engineering.
Authors: Fushimi, Tatsuya and Miura, Natsuko and Shintani, Hideya and Tsunoda, Hiroyuki and Kuroda, Kouichi and Ueda, Mitsuyoshi
Journal: Applied microbiology and biotechnology (2013): 4003-11

Termination of DNA synthesis by N6-alkylated, not 3′-O-alkylated, photocleavable 2′-deoxyadenosine triphosphates.
Authors: Wu, Weidong and Stupi, Brian P and Litosh, Vladislav A and Mansouri, Dena and Farley, Demetra and Morris, Sidney and Metzker, Sherry and Metzker, Michael L
Journal: Nucleic acids research (2007): 6339-49

5-Propargylamino-3′-azidomethyl-dCTP

	货号	17091	存储条件	在零下15度以下保存, 避免光照
	规格	50 nmoles	价格	3036
	Ex (nm)		Em (nm)
	分子量	575.26	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17091

产品名称：5-Propargylamino-3′-azidomethyl-dCTP

规格：50 nmoles

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：575.26

溶剂：水

产品介绍

5-Propargylamino-3′-azidomethyl-dCTP是制备用于下一代测序（NGS）的荧光缀合物的关键组成部分。 NGS使用与早期Sanger测序相似的链终止方法，但NGS是通过荧光标记的核苷酸类似物作为扩增反应的可逆终止剂来进行的。 NGS依赖于可逆的DNA聚合阻断，而Sanger测序则使用ddNTP不可逆转的DNA聚合阻断。 NGS的另一个不同特征是通过桥式PCR进行体外克隆扩增以增加待测序分子的数量。在此平台上，片段与固定在固体表面上的引物连接，进行原位扩增，生成具有相同分子的DNA簇。在每个循环中，同时添加可逆终止的四个核苷酸，并通过它们互补的聚合酶掺入。通过将3′-OH基团替换为3′-o-叠氮基甲基，这些核苷酸被化学封闭，以防止聚合酶在每个循环中掺入一个以上的核苷酸。掺入核苷酸后，在不同通道中针对不同碱基测量荧光信号。关于下一循环，洗涤未掺入的核苷酸，并用TCEP去除3’端的化学封锁。一旦收集到荧光信号，就会开始一个新的循环，重复此动态过程，直到完成每个片段的测序为止。总之，NGS测序反应分三个步骤进行：核苷酸的添加，成像和通过荧光团裂解的3′-OH再生。

参考文献

Genome-wide analysis of the specificity and mechanisms of replication infidelity driven by imbalanced dNTP pools.
Authors: Watt, Danielle L and Buckland, Robert J and Lujan, Scott A and Kunkel, Thomas A and Chabes, Andrei
Journal: Nucleic acids research (2016): 1669-80

Fluorescence polarization-based method with bisulfite conversion-specific one-label extension for quantification of single CpG dinucleotide methylation.
Authors: Li, Shufen and Wang, Zhongju and Zhou, Lin and Luo, Fu and Zhao, Cunyou
Journal: Genome (2015): 357-63

Hi-C in Budding Yeast.
Authors: Belton, Jon-Matthew and Dekker, Job
Journal: Cold Spring Harbor protocols (2015): 649-61

Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: assembly, annotation and molecular marker discovery.
Authors: Ahn, Yul-Kyun and Tripathi, Swati and Kim, Jeong-Ho and Cho, Young-Il and Lee, Hye-Eun and Kim, Do-Sun and Woo, Jong-Gyu and Cho, Myeong-Cheoul
Journal: Gene (2014): 494-9

Structures of an apo and a binary complex of an evolved archeal B family DNA polymerase capable of synthesising highly cy-dye labelled DNA.
Authors: Wynne, Samantha A and Pinheiro, Vitor B and Holliger, Philipp and Leslie, Andrew G W
Journal: PloS one (2013): e70892

7-Deaza-7-Propargylamino-3′-azidomethyl-dGTP

	货号	17092	存储条件	在零下15度以下保存, 避免光照
	规格	50 nmoles	价格	4272
	Ex (nm)		Em (nm)
	分子量	614.30	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17092

产品名称：7-Deaza-7-Propargylamino-3′-azidomethyl-dGTP

规格：50 nmoles

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：614.30

溶剂：水

产品介绍

7-Deaza-7-Propargylamino-3′-azidomethylmethyl-dGTP是制备用于下一代测序（NGS）的荧光缀合物的关键组成部分。 NGS使用与早期Sanger测序相似的链终止方法，但NGS是通过荧光标记的核苷酸类似物作为扩增反应的可逆终止剂来进行的。 NGS依赖于可逆的DNA聚合阻断，而Sanger测序则使用ddNTP不可逆转的DNA聚合阻断。 NGS的另一个不同特征是通过桥式PCR进行体外克隆扩增以增加待测序分子的数量。在此平台上，片段与固定在固体表面上的引物连接，进行原位扩增，生成具有相同分子的DNA簇。在每个循环中，同时添加可逆终止的四个核苷酸，并通过它们互补的聚合酶掺入。通过将3′-OH基团替换为3′-o-叠氮基甲基，这些核苷酸被化学封闭，以防止聚合酶在每个循环中掺入一个以上的核苷酸。掺入核苷酸后，在不同通道中针对不同碱基测量荧光信号。关于下一循环，洗涤未掺入的核苷酸，并用TCEP去除3’端的化学封锁。一旦收集到荧光信号，就会开始一个新的循环，重复此动态过程，直到完成每个片段的测序为止。总之，NGS测序反应分三个步骤进行：核苷酸的添加，成像和通过荧光团裂解的3′-OH再生。

参考文献

A novel target enrichment strategy in next-generation sequencing through 7-deaza-dGTP-resistant enzymatic digestion.
Authors: Peng, Peng and Xu, Yanjuan and Di Bisceglie, Adrian M and Fan, Xiaofeng
Journal: BMC research notes (2020): 445

SAMHD1-deficient fibroblasts from Aicardi-Goutières Syndrome patients can escape senescence and accumulate mutations.
Authors: Franzolin, Elisa and Coletta, Sara and Ferraro, Paola and Pontarin, Giovanna and D’Aronco, Giulia and Stevanoni, Martina and Palumbo, Elisa and Cagnin, Stefano and Bertoldi, Loris and Feltrin, Erika and Valle, Giorgio and Russo, Antonella and Bianchi, Vera and Rampazzo, Chiara
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2020): 631-647

Suppressors of dGTP Starvation in Escherichia coli.
Authors: Itsko, Mark and Schaaper, Roel M
Journal: Journal of bacteriology (2017)

Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR.
Authors: Castellanos-Rizaldos, Elena and Richardson, Katherine and Lin, Rui and Wu, Grant and Makrigiorgos, Mike G
Journal: Clinical chemistry (2015): 267-77

Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: assembly, annotation and molecular marker discovery.
Authors: Ahn, Yul-Kyun and Tripathi, Swati and Kim, Jeong-Ho and Cho, Young-Il and Lee, Hye-Eun and Kim, Do-Sun and Woo, Jong-Gyu and Cho, Myeong-Cheoul
Journal: Gene (2014): 494-9

Deep sequencing analysis of mutations resulting from the incorporation of dNTP analogs.
Authors: Petrie, Katherine L and Joyce, Gerald F
Journal: Nucleic acids research (2010): 8095-104

Design and synthesis of a photocleavable fluorescent nucleotide 3′-O-allyl-dGTP-PC-Bodipy-FL-510 as a reversible terminator for DNA sequencing by synthesis.
Authors: Meng, Qinglin and Kim, Dae Hyun and Bai, Xiaopeng and Bi, Lanrong and Turro, Nicholas J and Ju, Jingyue
Journal: The Journal of organic chemistry (2006): 3248-52

5-Propargylamino-3′-azidomethyl-dUTP

	货号	17093	存储条件	在零下15度以下保存, 避免光照
	规格	50 nmoles	价格	3036
	Ex (nm)		Em (nm)
	分子量	576.24	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17093

产品名称：5-Propargylamino-3′-azidomethyl-dUTP

规格：50 nmoles

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：576.24

溶剂：水

产品介绍

5-Propargylamino-3′-azidomethyl-dUTP是制备用于下一代测序（NGS）的荧光缀合物的关键组成部分。 NGS使用与早期Sanger测序相似的链终止方法，但NGS是通过荧光标记的核苷酸类似物作为扩增反应的可逆终止剂来进行的。 NGS依赖于可逆的DNA聚合阻断，而Sanger测序则使用ddNTP不可逆转的DNA聚合阻断。 NGS的另一个不同特征是通过桥式PCR进行体外克隆扩增以增加待测序分子的数量。在此平台上，片段与固定在固体表面上的引物连接，进行原位扩增，生成具有相同分子的DNA簇。在每个循环中，同时添加可逆终止的四个核苷酸，并通过它们互补的聚合酶掺入。通过将3′-OH基团替换为3′-o-叠氮基甲基，这些核苷酸被化学封闭，以防止聚合酶在每个循环中掺入一个以上的核苷酸。掺入核苷酸后，在不同通道中针对不同碱基测量荧光信号。关于下一循环，洗涤未掺入的核苷酸，并用TCEP去除3’端的化学封锁。一旦收集到荧光信号，就会开始一个新的循环，重复此动态过程，直到完成每个片段的测序为止。总之，NGS测序反应分三个步骤进行：核苷酸的添加，成像和通过荧光团裂解的3′-OH再生。

参考文献

Application of Next Generation Sequencing in Laboratory Medicine.
Authors: Zhong, Yiming and Xu, Feng and Wu, Jinhua and Schubert, Jeffrey and Li, Marilyn M
Journal: Annals of laboratory medicine (2021): 25-43

Current scenario of the genetic testing for rare neurological disorders exploiting next generation sequencing.
Authors: Di Resta, Chiara and Pipitone, Giovanni Battista and Carrera, Paola and Ferrari, Maurizio
Journal: Neural regeneration research (2021): 475-481

A New Type of Chronic Wound Infection after Wisdom Tooth Extraction: A Diagnostic Approach with 16S-rRNA Gene Analysis, Next-Generation Sequencing, and Bioinformatics.
Authors: Böttger, Sebastian and Zechel-Gran, Silke and Streckbein, Philipp and Knitschke, Michael and Hain, Torsten and Weigel, Markus and Wilbrand, Jan-Falco and Domann, Eugen and Howaldt, Hans-Peter and Attia, Sameh
Journal: Pathogens (Basel, Switzerland) (2020)

A novel Next-Generation Sequencing-based approach for concurrent detection of mitochondrial DNA copy number and mutation.
Authors: Zhou, Kaixiang and Mo, Qinqin and Guo, Shanshan and Liu, Yang and Yin, Chun and Ji, Xiaoying and Guo, Xu and Xing, Jinliang
Journal: The Journal of molecular diagnostics : JMD (2020)

Amplicon-Based Next-Generation Sequencing for Detection of Fungi in Formalin-Fixed, Paraffin-Embedded Tissues: Correlation with Histopathology and Clinical Applications.
Authors: Larkin, Paige M K and Lawson, Katy L and Contreras, Deisy A and Le, Catherine Q and Trejo, Marisol and Realegeno, Susan and Hilt, Evann E and Chandrasekaran, Sukantha and Garner, Omai B and Fishbein, Gregory A and Yang, Shangxin
Journal: The Journal of molecular diagnostics : JMD (2020): 1287-1293

Analytical performance evaluation of a commercial next generation sequencing liquid biopsy platform using plasma ctDNA, reference standards, and synthetic serial dilution samples derived from normal plasma.
Authors: Verma, Suman and Moore, Mathew W and Ringler, Rebecca and Ghosal, Abhisek and Horvath, Kyle and Naef, Theodore and Anvari, Sheri and Cotter, Philip D and Gunn, Shelly
Journal: BMC cancer (2020): 945

Author Correction: Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction.
Authors: Ahmed, Ibrahim and Tucci, Felicia A and Aflalo, Aure and Smith, Kenneth G C and Bashford-Rogers, Rachael J M
Journal: Scientific reports (2020): 17010

Automation of Amplicon-Based Library Preparation for Next-Generation Sequencing by Centrifugal Microfluidics.
Authors: Hess, Jacob Friedrich and Kotrová, Michaela and Calabrese, Silvia and Darzentas, Nikos and Hutzenlaub, Tobias and Zengerle, Roland and Brüggemann, Monika and Paust, Nils
Journal: Analytical chemistry (2020): 12833-12841

Characterization of the novel HLA-B*15:474 allele by next-generation sequencing.
Authors: Genebrier, Steve and Elsermans, Vincent and Texeraud, Emeric and Bertrand, Gerald and Renac, Virginie
Journal: HLA (2020)

Correction to: Malignant struma ovarii: next-generation sequencing of six cases revealed Nras, Braf, and Jak3 mutations.
Authors: Poli, Roberta and Scatolini, Maria and Grosso, Enrico and Maletta, Francesca and Gallo, Marco and Liscia, Daniele and Nelva, Anna and Cesario, Flora and Forte, Giuseppe and Metovic, Jasna and Volante, Marco and Arvat, Emanuela and Papotti, Mauro
Journal: Endocrine (2020)

肽底物DLDVPIPGRFDRRVpSVAAE（H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu-OH）

	货号	31700	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	1800
	Ex (nm)		Em (nm)
	分子量	2192.34	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：31700

产品名称：DLDVPIPGRFDRRVpSVAAE（H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu-OH）

规格：1mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：2192.34

溶剂：DMSO

产品介绍

DLDVPIPGRFDRRVpSVAAE（H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser（PO 3 H 2）-Val-Ala-Ala-Glu-OH）是非常好钙调神经磷酸酶的肽底物（蛋白质磷酸酶2B，PP2B）。该序列衍生自II型PKA调节亚基（RII），并在Ser残基上被磷酸化。钙调神经磷酸酶是一种钙依赖性丝氨酸/苏氨酸磷酸酶，参与多种信号传导途径。钙调磷酸酶在磷酸酶中是不同的，因为其活性需要钙，并且对阻断相关磷酸酶PP1A和PP2A的化合物的抑制作用不敏感。当前的常用技术通过测量释放的放射性磷酸盐或用孔雀石绿检测游离磷酸盐来定量钙调神经磷酸酶的活性。两种方法都涉及技术挑战并且具有不良特征。 AAT Bioquest开发了一些出色的磷酸盐检测法，可用于监测钙调神经磷酸酶对DLDVPIPGRFDRRVpSVAAE脱磷酸作用中磷酸盐的释放。 PhosphoWorks 比色磷酸盐测定试剂盒（21665）和PhosphoWorks 比色MESG磷酸盐测定试剂盒（21659）可用于磷酸盐的比色测量。为了获得更高的灵敏度，可以使用PhosphoWorks 荧光磷酸盐检测试剂盒（21660）对荧光粉的释放进行荧光定量。

图示

图1. DLDVPIPGRFDRRVpSVAAE（H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu -OH）是钙调神经磷酸酶（蛋白质磷酸酶2B，PP2B）的极佳肽底物。 AAT Bioquest开发了一些出色的磷酸盐测定法，可用于监测钙调神经磷酸酶对DLDVPIPGRFDRRVpSVAAE脱磷酸作用中磷酸盐的释放。 PhosphoWorks 比色磷酸盐测定试剂盒（21665）和PhosphoWorks 比色MESG磷酸盐测定试剂盒（21659）可用于磷酸盐的比色测量。为了获得更高的灵敏度，可以使用PhosphoWorks 荧光磷酸盐检测试剂盒（21660）对荧光的释放进行荧光定量。

参考文献

Genome-wide analysis of the specificity and mechanisms of replication infidelity driven by imbalanced dNTP pools.
Authors: Watt, Danielle L and Buckland, Robert J and Lujan, Scott A and Kunkel, Thomas A and Chabes, Andrei
Journal: Nucleic acids research (2016): 1669-80

Fluorescence polarization-based method with bisulfite conversion-specific one-label extension for quantification of single CpG dinucleotide methylation.
Authors: Li, Shufen and Wang, Zhongju and Zhou, Lin and Luo, Fu and Zhao, Cunyou
Journal: Genome (2015): 357-63

Hi-C in Budding Yeast.
Authors: Belton, Jon-Matthew and Dekker, Job
Journal: Cold Spring Harbor protocols (2015): 649-61

Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: assembly, annotation and molecular marker discovery.
Authors: Ahn, Yul-Kyun and Tripathi, Swati and Kim, Jeong-Ho and Cho, Young-Il and Lee, Hye-Eun and Kim, Do-Sun and Woo, Jong-Gyu and Cho, Myeong-Cheoul
Journal: Gene (2014): 494-9

Structures of an apo and a binary complex of an evolved archeal B family DNA polymerase capable of synthesising highly cy-dye labelled DNA.
Authors: Wynne, Samantha A and Pinheiro, Vitor B and Holliger, Philipp and Leslie, Andrew G W
Journal: PloS one (2013): e70892

肽底物Mca-YVADAPK(Dnp)-OH

	货号	13557	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	1800
	Ex (nm)	322	Em (nm)	381
	分子量	1145.15	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：13557

产品名称：Mca-YVADAPK(Dnp)-OH

规格：1 mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：1145.15

激发波长(nm):322

发射波长(nm):381

校正因子（280 nm）:0.3

溶剂：水

产品介绍

Mca-YVADAPK（Dnp）-OH是一种荧光肽底物，用于检测caspase-1 /白介素转换酶（ICE）和血管紧张素I转换酶2（ACE-2）。它的N端包含高荧光的7-甲氧基香豆素（MCA）荧光团，可通过荧光共振能量转移（暗FRET）有效地淬灭赖氨酸残基上的非荧光2,4-二硝基苯基。它可以用于测量caspase-1 /白介素转化酶（ICE）和血管紧张素I转化酶2（ACE-2）的活性，这些酶能够裂解荧光基团（MCA）和荧光素之间的酰胺键。淬灭剂组（DNP），导致荧光增强。

点击查看光谱

参考文献

ACE2 expression in adipose tissue is associated with COVID-19 cardio-metabolic risk factors and cell type composition.
Authors: El-Sayed Moustafa, Julia Sarah and Jackson, Anne U and Brotman, Sarah M and Guan, Li and Villicaňa, Sergio and Roberts, Amy L and Zito, Antonino and Bonnycastle, Lori and Erdos, Michael R and Narisu, Narisu and Stringham, Heather M and Welch, Ryan and Yan, Tingfen and Lakka, Timo and Parker, Stephen and Tuomilehto, Jaakko and Collins, Francis S and Pajukanta, Päivi and Boehnke, Michael and Koistinen, Heikki A and Laakso, Markku and Falchi, Mario and Bell, Jordana T and Scott, Laura J and Mohlke, Karen L and Small, Kerrin S
Journal: medRxiv : the preprint server for health sciences (2020)

ACE2 receptor polymorphism: Susceptibility to SARS-CoV-2, hypertension, multi-organ failure, and COVID-19 disease outcome.
Authors: Devaux, Christian A and Rolain, Jean-Marc and Raoult, Didier
Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (2020): 425-435

Analysis of the susceptibility of lung cancer patients to SARS-CoV-2 infection.
Authors: Kong, Qi and Xiang, Zhiguang and Wu, Yue and Gu, Yu and Guo, Jianguo and Geng, Fei
Journal: Molecular cancer (2020): 80

Animal models of mechanisms of SARS-CoV-2 infection and COVID-19 pathology.
Authors: Cleary, Simon J and Pitchford, Simon C and Amison, Richard T and Carrington, Robert and Robaina Cabrera, C Lorena and Magnen, Mélia and Looney, Mark R and Gray, Elaine and Page, Clive P
Journal: British journal of pharmacology (2020)

Autophagy as an emerging target for COVID-19: lessons from an old friend, chloroquine.
Authors: Bonam, Srinivasa Reddy and Muller, Sylviane and Bayry, Jagadeesh and Klionsky, Daniel J
Journal: Autophagy (2020): 1-7

Broad Host Range of SARS-CoV-2 Predicted by Comparative and Structural Analysis of ACE2 in Vertebrates.
Authors: Damas, Joana and Hughes, Graham M and Keough, Kathleen C and Painter, Corrie A and Persky, Nicole S and Corbo, Marco and Hiller, Michael and Koepfli, Klaus-Peter and Pfenning, Andreas R and Zhao, Huabin and Genereux, Diane P and Swofford, Ross and Pollard, Katherine S and Ryder, Oliver A and Nweeia, Martin T and Lindblad-Toh, Kerstin and Teeling, Emma C and Karlsson, Elinor K and Lewin, Harris A
Journal: bioRxiv : the preprint server for biology (2020)

Broad host range of SARS-CoV-2 predicted by comparative and structural analysis of ACE2 in vertebrates.
Authors: Damas, Joana and Hughes, Graham M and Keough, Kathleen C and Painter, Corrie A and Persky, Nicole S and Corbo, Marco and Hiller, Michael and Koepfli, Klaus-Peter and Pfenning, Andreas R and Zhao, Huabin and Genereux, Diane P and Swofford, Ross and Pollard, Katherine S and Ryder, Oliver A and Nweeia, Martin T and Lindblad-Toh, Kerstin and Teeling, Emma C and Karlsson, Elinor K and Lewin, Harris A
Journal: Proceedings of the National Academy of Sciences of the United States of America (2020): 22311-22322

Elevated FiO2 increases SARS-CoV-2 co-receptor expression in respiratory tract epithelium.
Authors: Myti, Despoina and Gunjak, Miša and Casado, Francisco and Khaghani Raziabad, Solmaz and Nardiello, Claudio and Vadász, István and Herold, Susanne and Pryhuber, Gloria and Seeger, Werner and Morty, Rory E
Journal: American journal of physiology. Lung cellular and molecular physiology (2020): L670-L674

Expression of SARS-CoV-2 Entry Molecules ACE2 and TMPRSS2 in the Gut of Patients With IBD.
Authors: Burgueño, Juan F and Reich, Adrian and Hazime, Hajar and Quintero, Maria A and Fernandez, Irina and Fritsch, Julia and Santander, Ana M and Brito, Nivis and Damas, Oriana M and Deshpande, Amar and Kerman, David H and Zhang, Lanyu and Gao, Zhen and Ban, Yuguang and Wang, Lily and Pignac-Kobinger, Judith and Abreu, Maria T
Journal: Inflammatory bowel diseases (2020): 797-808

Hypercytokinemia and Pathogen-Host Interaction in COVID-19.
Authors: Badawi, Alaa
Journal: Journal of inflammation research (2020): 255-261

DIG-HRP缀合物

	货号	11023	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	1800
	Ex (nm)		Em (nm)
	分子量		溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：11023

产品名称：DIG-HRP缀合物

规格：1 mg

储存条件：-15℃避光防潮

保质期：24个月

产品介绍

地高辛配基（DIG）缀合的辣根过氧化物酶（HRP）是用于ELISA和其他DIG缀合测定进行生物检测的出色工具。抗DIG抗体可选择性地与DIG结合，结合的HRP通过适当的HRP底物系统通过颜色，荧光或发光为检测提供酶活性。该产品主要用于抗DIG抗体的一致性测量，主要用于ELISA应用。

参考文献

Genosensors for differential detection of Zika virus.
Authors: Alzate, Daniel and Cajigas, Sebastian and Robledo, Sara and Muskus, Carlos and Orozco, Jahir
Journal: Talanta (2020): 120648

Responsive surface bioaffinity binding to construct flexible and sensitive electrochemical aptasensors.
Authors: Liu, Ying and Zhu, Zhencai and Wang, Chao and Gao, Rui and Yang, Xiaoyan and Liu, Shufeng
Journal: The Analyst (2019): 2130-2137

Designing a new biosensor “DNA ELISA” to detect Escherichia coli using genomic DNA and comparison of this method to PCR-ELISA.
Authors: Hashemi, Elaheh and Forouzandeh, Mehdi
Journal: Journal of enzyme inhibition and medicinal chemistry (2018): 722-725

Accurate Electrochemistry Analysis of Circulating Methylated DNA from Clinical Plasma Based on Paired-End Tagging and Amplifications.
Authors: Chen, Feng and Wang, Xuyao and Cao, Xiaowen and Zhao, Yongxi
Journal: Analytical chemistry (2017): 10468-10473

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification.
Authors: Jandura, Allison and Hu, Jack and Wilk, Ronit and Krause, Henry M
Journal: Journal of visualized experiments : JoVE (2017)

TFDP3 was expressed in coordination with E2F1 to inhibit E2F1-mediated apoptosis in prostate cancer.
Authors: Ma, Yueyun and Xin, Yijuan and Li, Rui and Wang, Zhe and Yue, Qiaohong and Xiao, Fengjing and Hao, Xiaoke
Journal: Gene (2014): 253-9

An enzyme substrate binding aptamer complex based time-resolved fluorescence sensor for the adenosine deaminase detection.
Authors: Zhang, Kai and Yang, Qianlu and Zhang, Jue and Fu, Lixin and Zhou, Yan and Wu, Bing and Xie, Minhao and Huang, Biao
Journal: Biosensors & bioelectronics (2013): 87-92

Radioactive in situ hybridization for detecting diverse gene expression patterns in tissue.
Authors: Chen, Chun-Chun and Wada, Kazuhiro and Jarvis, Erich D
Journal: Journal of visualized experiments : JoVE (2012)

A novel homogeneous Biotin-digoxigenin based assay for the detection of human anti-therapeutic antibodies in autoimmune serum.
Authors: Qiu, Zhihua Julia and Ying, Yong and Fox, Michael and Peng, Kun and Lewin-Koh, Sock-Cheng and Coleman, Daniel and Good, Jeremy and Lowe, John and Rahman, Amena and Yang, Jihong and Jiang, Jenny and Quarmby, Valerie and Song, An
Journal: Journal of immunological methods (2010): 101-11

Double-tagging polymerase chain reaction with a thiolated primer and electrochemical genosensing based on gold nanocomposite sensor for food safety.
Authors: Marques, Paulo R Brasil de Oliveira and Lermo, Anabel and Campoy, Susana and Yamanaka, Hideko and Barbé, Jordi and Alegret, Salvador and Pividori, M Isabel
Journal: Analytical chemistry (2009): 1332-9

ddCTP [2′,3′-Dideoxycytidine-5′-triphosphate]

	货号	17207	存储条件	在零下15度以下保存, 避免光照
	规格	1 umole	价格	2412
	Ex (nm)		Em (nm)
	分子量	451.16	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17207

产品名称：ddCTP [2′,3′-Dideoxycytidine-5′-triphosphate]

规格：1 umole

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：451.16

溶剂：水

产品介绍

Sanger测序，也称为链终止法，是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸（ddNTPs）的DNA测序技术。它是由Frederick Sanger及其同事于1977年开发的。尽管新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍，但Sanger测序仍具有99.99％的准确度，仍然是临床的“黄金标准”研究排序。 dd-CTP是执行Sanger测序的四个关键ddNTP组件之一。

参考文献

Involvement of DNA polymerase beta in DNA replication and mutagenic consequences.
Authors: Servant, Laurence and Bieth, Anne and Hayakawa, Hiroshi and Cazaux, Christophe and Hoffmann, Jean-Sébastien
Journal: Journal of molecular biology (2002): 1039-47

Fluorescence-based DHPLC for allelic quantification by single-nucleotide primer extension.
Authors: Kosaki, K and Yoshihashi, H and Ohashi, Y and Kosaki, R and Suzuki, T and Matsuo, N
Journal: Journal of biochemical and biophysical methods (2001): 111-9

Denaturation fingerprinting: two related mutation detection methods especially advantageous for high G + C regions.
Authors: Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S
Journal: BioTechniques (1998): 140-7

Synergistic inhibition of HIV-1 reverse transcriptase by combinations of chain-terminating nucleotides.
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31

A fidelity assay using “dideoxy” DNA sequencing: a measurement of sequence dependence and frequency of forming 5-bromouracil X guanine base mispairs.
Authors: Lasken, R S and Goodman, M F
Journal: Proceedings of the National Academy of Sciences of the United States of America (1985): 1301-5

ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate]

	货号	17208	存储条件	在零下15度以下保存, 避免光照
	规格	1 umole	价格	2412
	Ex (nm)		Em (nm)
	分子量	466.17	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17208

产品名称：ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate]

规格：1 umole

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：466.17

溶剂：水

产品介绍

Sanger测序，也称为链终止法，是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸（ddNTPs）的DNA测序技术。它是由Frederick Sanger及其同事于1977年开发的。尽管新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍，但Sanger测序的准确度仍为99.99％，仍然是临床的“黄金标准”研究排序。 dd-TTP是执行Sanger测序的四个关键ddNTP组件之一。

参考文献

Strong positional preference in the interaction of LNA oligonucleotides with DNA polymerase and proofreading exonuclease activities: implications for genotyping assays.
Authors: Di Giusto, Daniel A and King, Garry C
Journal: Nucleic acids research (2004): e32

Detection and genotyping of human papillomavirus DNA in cervical cancer tissues with fluorescence polarization.
Authors: Gao, Yan-E and Zhang, Ju and Wu, Jing and Chen, Zhong-Can and Yan, Xiao-Jun
Journal: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica (2003): 1029-34

Dye structure affects Taq DNA polymerase terminator selectivity.
Authors: Brandis, J W
Journal: Nucleic acids research (1999): 1912-8

Synergistic inhibition of HIV-1 reverse transcriptase by combinations of chain-terminating nucleotides.
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31

Characterization of the p68/p58 heterodimer of human immunodeficiency virus type 2 reverse transcriptase.
Authors: Fan, N and Rank, K B and Poppe, S M and Tarpley, W G and Sharma, S K
Journal: Biochemistry (1996): 1911-7

Mechanism of inhibition of DNA synthesis by 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil.
Authors: Suzutani, T and Machida, H and Honess, R W
Journal: Microbiology and immunology (1993): 511-3

Expression of enzymatically active reverse transcriptase of simian immunodeficiency virus in bacteria: sensitivity to nucleotide analogue inhibitors.
Authors: Prasad, V R and Myrick, K and Haseltine, W and Goff, S P
Journal: Virology (1990): 896-900

Wheat embryo DNA polymerase A reverse transcribes natural and synthetic RNA templates. Biochemical characterization and comparison with animal DNA polymerase gamma and retroviral reverse transcriptase.
Authors: Laquel, P and Sallafranque-Andreola, M and Tarrago-Litvak, L and Castroviejo, M and Litvak, S
Journal: Biochimica et biophysica acta (1990): 139-48

3′-Deoxy-3′-fluorothymidinetriphosphate: inhibitor and terminator of DNA synthesis catalysed by DNA polymerase beta, terminal deoxynucleotidyl transferase and DNA polymerase I.
Authors: Matthes, E and Lehmann, C and Drescher, B and Büttner, W and Langen, P
Journal: Biomedica biochimica acta (1985): K63-73

A fidelity assay using “dideoxy” DNA sequencing: a measurement of sequence dependence and frequency of forming 5-bromouracil X guanine base mispairs.
Authors: Lasken, R S and Goodman, M F
Journal: Proceedings of the National Academy of Sciences of the United States of America (1985): 1301-5

ddATP [2′,3′-Dideoxyadenosine-5′-triphosphate]

	货号	17209	存储条件	在零下15度以下保存, 避免光照
	规格	1 umole	价格	2412
	Ex (nm)		Em (nm)
	分子量	475.18	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17209

产品名称：ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate]

规格：1 umole

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：475.18

溶剂：水

产品介绍

Sanger测序，也称为链终止法，是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸（ddNTPs）的DNA测序技术。它是由Frederick Sanger及其同事于1977年开发的。尽管新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍，但Sanger测序的准确度仍为99.99％，仍然是临床的“黄金标准”研究排序。 dd-ATP是执行Sanger测序的四个关键ddNTP组件之一。

参考文献

Denaturation fingerprinting: two related mutation detection methods especially advantageous for high G + C regions.
Authors: Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S
Journal: BioTechniques (1998): 140-7

Slow rate of phosphodiester bond formation accounts for the strong bias that Taq DNA polymerase shows against 2′,3′-dideoxynucleotide terminators.
Authors: Brandis, J W and Edwards, S G and Johnson, K A
Journal: Biochemistry (1996): 2189-200

Recombinant human hepatitis B virus reverse transcriptase is active in the absence of the nucleocapsid or the viral replication origin, DR1.
Authors: Seifer, M and Standring, D N
Journal: Journal of virology (1993): 4513-20

The enzymatic termination of polydeoxynucleotides by 2′,3′-dideoxyadenosine triphosphate.
Authors: Toji, L and Cohen, S S
Journal: Proceedings of the National Academy of Sciences of the United States of America (1969): 871-7

ddGTP [2′,3′-Dideoxyguanosine-5′-triphosphate]

	货号	17210	存储条件	在零下15度以下保存, 避免光照
	规格	1 umole	价格	2412
	Ex (nm)		Em (nm)
	分子量	491.18	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17210

产品名称：ddGTP [2′,3′-Dideoxyguanosine-5′-triphosphate]

规格：1 umole

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：491.18

溶剂：水

产品介绍

Sanger测序，也称为链终止法，是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸（ddNTPs）的DNA测序技术。它是由Frederick Sanger及其同事于1977年开发的。尽管新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍，但Sanger测序仍具有99.99％的准确度，仍然是临床的“黄金标准”研究排序。 dd-GTP是执行Sanger测序的四个关键ddNTP组件之一。

参考文献

Detection and genotyping of human papillomavirus DNA in cervical cancer tissues with fluorescence polarization.
Authors: Gao, Yan-E and Zhang, Ju and Wu, Jing and Chen, Zhong-Can and Yan, Xiao-Jun
Journal: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica (2003): 1029-34

Microcapillary reactors using solid-phase DNA sequencing for direct sample introduction into slab gels.
Authors: Xu, Y and Bruch, R C and Soper, S A
Journal: BioTechniques (2000): 904-8, 910, 912

Isolation and characterization of a dideoxyguanosine triphosphate-resistant mutant of human immunodeficiency virus reverse transcriptase.
Authors: Prasad, V R and Lowy, I and de los Santos, T and Chiang, L and Goff, S P
Journal: Proceedings of the National Academy of Sciences of the United States of America (1991): 11363-7

Herpes simplex virus type 1 and human DNA polymerase interactions with 2′-deoxyguanosine 5′-triphosphate analogues. Kinetics of incorporation into DNA and induction of inhibition.
Authors: Reardon, J E
Journal: The Journal of biological chemistry (1989): 19039-44

荧光底物Beta-Ala-AMC

	货号	13202	存储条件	在零下15度以下保存, 避免光照
	规格	5 mg	价格	1176
	Ex (nm)	341	Em (nm)	441
	分子量	282.72	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：13202

产品名称：Beta-Ala-AMC

规格：5 mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：282.72

激发波长(nm):341

发射波长(nm):441

溶剂：DMSO

产品介绍

Beta-Ala-AMC是一种荧光底物，可用于检测胰腺弹性蛋白酶，芳基酰胺酶和β-丙氨酸氨基肽酶活性。它也可以用于检测具有β-丙氨酸氨基肽酶活性的铜绿假单胞菌。在沙雷氏菌，铜绿假单胞菌，荧光假单胞菌，洋葱伯克霍尔德菌，mendocina假单胞菌和拟南芥中检测到β-丙氨酸氨基肽酶活性。因此，β-Ala-AMC可用于检测食品和其他样品中的这些物种。

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