qiagen 74903植物总RNA提取试剂盒RNeasy Plant Mini Kit Print
RNeasy Plant Mini Kit含QIAshredder离心柱和RNeasy离心柱，前者用于匀质化和过滤高粘度的植物或真菌的裂解物，后者使用硅胶膜技术纯化至多100 μg高品质RNA。纯化过程可在QIAcube全自动核酸纯化仪上自动化进行。该试剂盒也可配合TissueRuptor或TissueLyser体系使用，高效破碎并匀质化植物样本。
qiagen 74903植物总RNA提取试剂盒RNeasy Plant Mini Kit Print
erformance
RNeasy Plant Mini Kit适用于从各种植物和真菌样本中纯化总RNA，样本为10–100 mg的组织或100–1 x 107个细胞（参见”RNeasy Plant Mini Kit处理的样本”）。离心柱的结合能力达100 µg RNA。100 mg植物组织的常规产量是25–65 µg RNA，但样本不同的发育阶段和生长条件可能导致不同的RNA产量（参见”100 mg组织的产量”）。
RNeasy Plus Micro Kit能从多达5 x 105个细胞或5 mg组织中分离总RNA，RNeasy Plus Mini Kit能够从107个细胞或30 mg组织中分离总RNA。简洁的工作流程可在25分钟内纯化得到已去除基因组DNA的RNA。样本首先被裂解和匀质化。裂解液流过gDNA Eliminator柱，在流过gDNA Eliminator柱的溶液中加入乙醇，样本再上样到RNeasy离心柱。RNA结合到膜上，污染物被洗去。获得高品质RNA，RNeasy Plus Micro Kit的洗脱体积为14 µl，RNeasy Plus Mini Kit的洗脱体积为30 µl。
使用Buffer RLT Plus（RNeasy Plus Micro Kit提供）进行组织破碎和匀质化时，可能会产生过多泡沫。在破碎和匀浆前向Buffer RLT Plus中加入Reagent DX（单独提供）可充分减少泡沫产生。Reagent DX经仔细检测，表明配合此试剂盒使用不会影响RNA的纯度或下游应用。
qiagen 74136DNA微量提取试剂盒
For purification of up to 100 µg total RNA from cells/tissues using gDNA Eliminator columns
Unique gDNA Eliminator columns avoid the need for DNase
Efficient removal of genomic DNA
Highly reproducible yields of RNA in minutes
High-performance RNA for sensitive applications
The RNeasy Plus Mini Kit integrates fast, convenient purification of up to 100 µg RNA with effective elimination of genomic DNA. Cell or tissue lysates are spun through gDNA Eliminator spin columns to remove genomic DNA. Total RNA is then purified using RNeasy Mini spin columns. The kit can be automated on theQIAcube. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. For smaller samples, the RNeasy Plus Micro Kit (spin-column binding capacity of 45 µg RNA) is also available.
详细描述： The discs large (dlg) tumor suppressor gene was first identified in Drosophila through genetic analysis of germline mutations. Several mammalian homologs were subsequently identified and categorized into a protein family designated MAGUK (membrane-associa
RNeasy Plus Kits将快速方便的RNA纯化步骤和有效去除基因组DNA污染整合在一起。使用该试剂盒能够从多种培养细胞和易裂解组织中纯化获得高纯度、高产量RNA。RNeasy Plus Mini Kit 一次能够纯化获得100 µg总RNA（>200 nt），并且高效去除基因组DNA。RNeasy Plus Micro Kit则适用于样本量较少的情况，可分离获得45 µg总RNA。该试剂盒可在QIAcube全自动核酸纯化仪上实现自动操作，可用RNAlater RNA Stabilization Reagent或Allprotect Tissue Reagent方便的稳定组织样本。
qiagen货号74134 RNeasy Plus Mini Kit
订购信息：
Cat No./ID: 74134
RNeasy Plus Mini Kit (50)
For 50 minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Spin Columns, Collection Tubes, RNase-Free Water and Buffers
Cat No./ID: 74136
RNeasy Plus Mini Kit (250)
For 250 minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Spin Columns, Collection Tubes, RNase-Free Water and Buffers
使用RNeasy Mini Kit纯化的总RNA具有高质量，适用于多种下游操作。实验方案还包括部分纯化的RNA、体外转录本和酶反应RNA的回收。不提供溶壁酶、酵母裂解酶或玻璃珠（酵母样本需要）。因样本来源的发育阶段、种属和生长条件的不同，从样本中分离的RNA量也各异。由于RNA酶步骤富集的RNA种属>200 nt，因此RNA产量不包括5S rRNA、tRNA或其他低分子量RNA。
使用RNeasy Mini Kit从图示数目的HeLa细胞中分离的总RNA的RT-PCR。使用不含RNA酶的DNA酶酶切10 µl（1/5）洗脱液，并使用oligo-dT引物进行逆转录。使用2.5 µl（1/20）的cDNA混合物进行50 µl PCR。扩增452 bp的GAPDH片段。C-：阴性对照；C+：阳性对照；M：100 bp分子量标准。
Performance
RNeasy Mini Kit可从至多30 mg动物或人类组织样本，或从100–1 x 107个动物或人类细胞样本或酵母中纯化总RNA（参见”RT-PCR of RNA from as few as 100 cells”和”High-quality RNA from a variety of samples”）。
详细描述： The discs large (dlg) tumor suppressor gene was first identified in Drosophila through genetic analysis of germline mutations. Several mammalian homologs were subsequently identified and categorized into a protein family designated MAGUK (membrane-associa
PHAGOTEST® is a complete diagnostic kit for the investigation of the phagocytic function of granulocytes and monocytes [1, 2] in whole blood. This test kit allows the quantitative determination of leukocyte phagocytosis in heparinized whole blood. It contains fluorescein (FITC) labelled opsonized bacteria (E. coli-FITC) and necessary reagents. It measures the overall percentage of monocytes and granulocytes showing phagocytosis in general (ingestion of one or more bacteria per cell) and the individual cellular phagocytic activity (number of bacteria per cell). The evaluation of the results can be done either by flow cytometry or by fluorescence microscopy.
· Evaluation of single cells to detect heterogenous populations
评价检测单个细胞异质种群
· Whole blood assay: No isolation procedures and optimal culture medium
全血检测：没有隔离程序和培养基
· Physiological stimulans for phagocytes: Bacteria and fMLP
吞噬细胞生理：细菌和fMLP
· Dose response: Low and high stimulant
剂量反应:低和高的
· No electrostatic artifacts in polystyrol tubes compared to latex beads
没有静电工件比较乳胶珠子聚苯乙烯管内
· Standardized test procedure
标准化测试程序
· Exclusion of aggregation artifacts by DNA staining
通过DNA染色排除聚合工件
· Compatible with whole blood of mice and rats
兼容的小鼠和大鼠全血
· Fast assay: Whole assay time is 1.5 hours
快速测定:整个试验时间是1.5小时
100 analyses. Clinical Diagnostic for the Quantitative Analysis of Leukocyte Oxidative Burst in Human Whole Blood. Evaluation by flow cytometry.
100次分析。临床诊断人体全血白细胞氧化破裂的定量检测。通过流式细胞术进行评估。
Clinical Diagnostic for the Quantitative Analysis of Leukocyte Oxidative Burst in Human Whole Bloods
GLYCOTOPE公司PHAGOBURST白细胞氧化突发定量分析10-0200
SUMMARY and EXPLANATION
BURSTTEST (PHAGOBURST) allows the quantitative determination of leukocyte oxidative burst in heparinized whole blood. It contains unlabeled opsonized bacteria (E.coli), phorbol 12-myristate 13-acetate (PMA) and the chemotactic peptide N-formyl-Met-Leu-Phe (fMLP) as stimulants, Dihydrorhodamine (DHR) 123 as a fluorogenic substrate and necessary reagents. It determines the percentage of phagocytic cells which produce reactive oxidants (conversion of DHR 123 to R 123) and their enzymatic activity (amount of R 123 per cell).
The evaluation of these bioactivities should be performed by flow cytometry.
BURSTTEST(PHAGOBURST)允许定量测定白细胞氧化闯入肝素化全血。 它包含未标记的促进调理作用的细菌(大肠杆菌),佛波醇12十四烷酸乙酸13(PMA)和趋化作用的多肽n甲酰遇见亮氨酸板式换热器(fMLP)作为xing奋剂,二氢若丹明(DHR)123作为一个荧光衬底和必要的试剂。它决定了吞噬细胞产生活性氧化剂的比例(转换DHR 123 到 R 1233)及其酶活性(每个细胞R 123的数量)。
这些生物活性的测定应该有流式细胞术完成。
APPLICATIONS
The diagnostic kit is intended to investigate the altered oxidative burst activity found in various disorders and to evaluate the effects of drugs.
Reduced or missing burst activity is observed in inborne defects like the chronic granulomatous disease (CGD). CGD is a heterogenous group of inherited disorders that usually manifests itself during the first two years of life (3, 4). The disease is characterized clinically by repeated and life-threatening infections caused by bacterial and fungal organisms. These infections typically consist of pneumonia, lymphadenitis, or abscesses that involve lymph nodes, lungs, and liver. The NADPH oxidase is the enzyme system responsible for producing superoxide anion, which is quickly converted to hydrogen peroxide and hydroxyl radicals. Abnormalities in the constituent peptides of the NADPH oxidase enzyme system lead to the dysfunctions characteristic of CGD. Neutrophils from CGD patients fail to produce a significant oxidative burst following stimulation. Different forms of CGD are described (classical X-linked CGD and autosomal recessive patterns). BURSTTEST (PHAGOBURST?) is a rapid and sensitive method for the diagnosis of CGD and for the detection of X-linked carriers.
The oxidative burst of granulocytes is impaired in transplant patients and patients with AIDS (6). The spontaneous and fMLP-induced neutrophil respiratory burst was shown to be increased in neonates with laboratory signs of infection (7). Various immunomodulators (e.g., cytokines (GM-CSF, G-CSF, TNF) or drugs) seem to have effects on the oxidative burst. By using fMLP as a low stimulant one can investigate additive or priming effects (8) of test substances.
The diagnostic kit is also applicable on blood of mice, rats, rabbits, dogs, cattle and other species.
TEST PRINCIPLES Phagocytosis by polymorphonuclear neutrophils and monocytes constitutes an essential arm of host defense against bacterial or fungal infections. The phagocytic process can be separated into several major stages: chemotaxis (migration of phagocytes to inflammatory sites), attachment of particles to the cell surface of phagocytes, ingestion (phagocytosis) and intracellular killing by oxygen-dependent (oxidative burst) and oxygen-independent mechanisms (1, 2).
BURSTTEST (PHAGOBURST?) allows the quantitative determination of leukocyte oxidative burst. The BURSTEST kit contains unlabelled opsonized E.coli bacteria as particulate stimulus, the protein kinase C ligand phorbol 12-myristate 13-acetate (PMA) as high stimulus and the chemotactic peptide N-formyl-MetLeuPhe (fMLP) as low physiological stimulus, Dihydrorhodamine (DHR) 123 as a fluorogenic substrate (5) and necessary reagents. Heparinized whole blood is incubated with the various stimuli at 37°C, a sample without stimulus serves as negative background control. Upon stimulation, granulocytes and monocytes produce reactive oxygen metabolites (superoxide anion, hydrogen peroxide, hypochlorous acid) which destroy bacteria inside the phagosome. Formation of the reactive oxidants during the oxidative burst can be monitored by the addition and oxidation of DHR 123. The reaction is stopped by addition of LYSING SOLUTION, which removes erythrocytes and results in a partial fixation of leukocytes. After one washing step with WASHING SOLUTION, DNA STAINING SOLUTION is added to exclude aggregation artifacts of bacteria or cells. The percentage of cells having produced reactive oxygen radicals are then analyzed as well as their mean fluorescence intensity (enzymatic activity)
qiagen 73404 RNeasy Plus Universal Tissue Mini Kit
qiagen 73404 RNeasy Plus Universal Tissue Mini Kit
从各种类型组织中纯化总RNA，配有gDNA Eliminator Solution
适用于所有组织溶液类型
整合RNeasy和QIAzol技术，节省时间
不使用酶，快速去除基因组DNA
RNA纯度高，适用于下游应用
RNeasy Plus Universal Kits可快速、方便的纯化RNA，同时有效地去除基因组DNA。优化的实验方案可从各种组织类型，包括难裂解组织中纯化高品质RNA。该试剂盒含有QIAzol Lysis Reagent和RNeasy离心柱，前者用于裂解脂肪组织和其他组织类型，后者用于纯化高品质RNA。该试剂盒有两种规格：RNeasy Plus Universal Mini Kit可从至多50 mg组织中纯化RNA，RNeasy Plus Universal Midi Kit可从至多250 mg组织中纯化RNA。RNeasy Plus Universal Mini Kit可在QIAcube全自动核酸纯化仪上自动化进行纯化。
订购信息：
Cat No./ID: 73404
RNeasy Plus Universal Mini Kit (50)
Log in
For 50 RNA minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Solution, Collection Tubes, RNase-Free Water and Buffers
Cat No./ID: 73442
RNeasy Plus Universal Midi Kit (10)
Log in
For 10 RNA midipreps: RNeasy Midi Spin Columns, gDNA Eliminator Solution, Collection Tubes, RNase-Free Water and Buffers
invitrogen MPK1096 Neon Transfection System 10 µL Kit
描述
The Neon® Transfection System 10 µL Kit is designed specifically for use with the Neon® Transfection System. Use this kit for transfection volumes of 10 µL, containing 5 × 104–2 × 105adherent cells or 1 × 105–5 × 105 suspension cells. Cells that have been transfected using the included 10 µL Neon® Tips are ready to be washed and plated into a multi-well culture dish
invitrogen MPK1096 Neon Transfection System 10 µL Kit
规格
Cell Type:
Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells
详细描述： The discs large (dlg) tumor suppressor gene was first identified in Drosophila through genetic analysis of germline mutations. Several mammalian homologs were subsequently identified and categorized into a protein family designated MAGUK (membrane-associa
Anti-Mouse MIF Pre-coated 96 well Strip Microplate
Mouse MIF Detection Antibody
Mouse MIF Standard
Avidin-HRP
Assay Buffer B
Wash Buffer (20X)
Substrate Solution F
Stop Solution
Plate Sealers
biolegend 444107 mouse MIF elisa kit代理
Product Details
Application:
ELISA
Standard Range:
156 – 10,000 pg/mL
Sensitivity:
25.28 ± 1.68 pg/mL
Description:
Macrophage migration inhibitory factor (MIF), also known as glycosylation inhibiting factor (GIF), is a pituitary hormone and a macrophage- and T cell-derived cytokine that plays an important role in the endocrine and immune systems. MIF consists of 115 amino acids and has a molecular weight of 12.5 kD. Mouse MIF shares most of its identity with human (90%), rat (99%), and cattle (88%) MIFs, and a moderate percentage of its identity with MIFs from a wide variety of other species. It exists in monomeric, dimeric, and trimeric forms of identical protomers.
MIF is active as a pro-inflammatory regulator and upregulates multiple cytokines that are vital to combating infections in vivo, including tumor-necrosis factor (TNF), interferon-γ (IFN-γ), and interleukin-12 (IL-12). It is known to maintain cell survival and promote pro-inflammatory immune responses. MIF also activates the ERK1/ERK2 signaling pathway, which inhibits the immunosuppressive effects of glucocorticoids. MIF also plays a critical role in superantigen-induced toxic shock. It upregulates several factors that enhance the growth and invasiveness of gram-negative bacteria. MIF can enhance many inflammatory diseases, such as acute respiratory distress syndrome, arthritis, atopic dermatitis, allograft rejection, glomerulonephritis, inflammatory bowel diseases, asthma, and atherosclerosis. Known receptors for MIF include CXCR2, CXCR4, CD44, and CD74.
The LEGEND MAX™ Mouse MIF ELISA kit is a ready-to-use Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a rat monoclonal anti-mouse MIF antibody. The Detection Antibody is a biotinylated goat polyclonal anti-mouse MIF antibody. This assay is specifically designed to quantify mouse MIF from serum, plasma, cell culture supernatant, and cell lysate. This kit is analytically validated with prepared reagents.
