|
品牌：FUJIFILM Wako
CAS No.： 储存条件：室温 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
232-02661 |
– | 1 column(W) | – | 7,410.00 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。
NEBuffer 1.1: 75%
NEBuffer 2.1: 100%
NEBuffer 3.1: 100%
CutSmart Buffer: 100%
重组酶、省时酶。
NEBuffer 3.1，37℃。
20,000 和 100,000 units/ml。
对 dam、dcm 和哺乳动物 CpG 甲基化均不敏感。
甘油浓度 ＞ 5% 条件下可能出现星号活性。
lumiprobe荧光染料官网代理商 水溶性荧光染料代理商 脂溶性荧光染料代理商
| Cat. # | Quantity | Price | Lead time | Buy this product | ||
|---|---|---|---|---|---|---|
| 13420 | 1 mg | 110.00$
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| 43420 | 25 mg | 410.00$
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| 53420 | 50 mg | 695.00$
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| 63420 | 100 mg | 1190.00$
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BDP TR is a bright and photostable borondipyrromethene dye, which is especially suitable for microscopy applications. The dye has a long excited state lifetime, and is therefore is useful for fluorescence polarization assays. Its large two-photon cross section makes this dye useful for two-photon spectroscopy.
This NHS ester is an amine-reactive form of the dye.
Download in
ChemDraw format
Water soluble far red fluorescent dye sulfo-Cyanine5.5, free non-activated carboxylic acid. Useful for the calibration, and as a non-reactive control.
Hampton Research蛋白结晶试剂盒
Products > Custom Shop Crystallization Reagents > StockOptions pH > Individual StockOptions pH Reagents
HR2-941-**
** Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-941-01
Click to Zoom In
HR2-941-01
StockOptions pH 01 185ml
1.0 M Citric acid pH 2.2
$181.00
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HR2-941-02
StockOptions pH 02 185ml
1.0 M Citric acid pH 2.4
$181.00
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HR2-941-03
StockOptions pH 03 185ml
1.0 M Citric acid pH 2.6
$181.00
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HR2-941-04
StockOptions pH 04 185ml
1.0 M Citric acid pH 2.8
$181.00
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HR2-941-05
StockOptions pH 05 185ml
1.0 M Citric acid pH 3.0
$181.00
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HR2-941-06
StockOptions pH 06 185ml
1.0 M Citric acid pH 3.2
$181.00
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HR2-941-07
StockOptions pH 07 185ml
1.0 M Citric acid pH 3.4
$181.00
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HR2-941-08
StockOptions pH 08 185ml
1.0 M Citric acid pH 3.6
$181.00
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HR2-941-09
StockOptions pH 09 185ml
1.0 M Citric acid pH 3.8
$181.00
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HR2-941-10
StockOptions pH 10 185ml
1.0 M Sodium acetate trihydrate pH 4.0
$185.00
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HR2-941-11
StockOptions pH 11 185ml
1.0 M Sodium acetate trihydrate pH 4.2
$185.00
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HR2-941-12
StockOptions pH 12 185ml
1.0 M Sodium acetate trihydrate pH 4.4
$185.00
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HR2-941-13
StockOptions pH 13 185ml
1.0 M Sodium acetate trihydrate pH 4.6
$185.00
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HR2-941-14
StockOptions pH 14 185ml
1.0 M Sodium acetate trihydrate pH 4.8
$185.00
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HR2-941-15
StockOptions pH 15 185ml
1.0 M Sodium citrate tribasic dihydrate pH 5.0
$205.00
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HR2-941-16
StockOptions pH 16 185ml
1.0 M Sodium citrate tribasic dihydrate pH 5.2
$205.00
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HR2-941-17
StockOptions pH 17 185ml
1.0 M Sodium citrate tribasic dihydrate pH 5.4
$205.00
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HR2-941-18
StockOptions pH 18 185ml
1.0 M Sodium citrate tribasic dihydrate pH 5.6
$205.00
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HR2-941-19
StockOptions pH 19 185ml
1.0 M Sodium citrate tribasic dihydrate pH 5.8
$205.00
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HR2-941-20
StockOptions pH 20 185ml
1.0 M Sodium cacodylate trihydrate pH 6.0
$266.00
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HR2-941-21
StockOptions pH 21 185ml
1.0 M Sodium cacodylate trihydrate pH 6.2
$266.00
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HR2-941-22
StockOptions pH 22 185ml
1.0 M Sodium cacodylate trihydrate pH 6.4
$266.00
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HR2-941-23
StockOptions pH 23 185ml
1.0 M Sodium cacodylate trihydrate pH 6.6
$266.00
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HR2-941-24
StockOptions pH 24 185ml
1.0 M Sodium cacodylate trihydrate pH 6.8
$266.00
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HR2-941-25
StockOptions pH 25 185ml
1.0 M HEPES sodium pH 7.0
$231.00
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HR2-941-26
StockOptions pH 26 185ml
1.0 M HEPES sodium pH 7.2
$231.00
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HR2-941-27
StockOptions pH 27 185ml
1.0 M HEPES sodium pH 7.4
$231.00
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HR2-941-28
StockOptions pH 28 185ml
1.0 M HEPES sodium pH 7.6
$231.00
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HR2-941-29
StockOptions pH 29 185ml
1.0 M HEPES sodium pH 7.8
$231.00
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HR2-941-30
StockOptions pH 30 185ml
1.0 M TRIS hydrochloride pH 8.0
$176.00
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HR2-941-31
StockOptions pH 31 185ml
1.0 M TRIS hydrochloride pH 8.2
$176.00
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HR2-941-32
StockOptions pH 32 185ml
1.0 M TRIS hydrochloride pH 8.4
$176.00
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HR2-941-33
StockOptions pH 33 185ml
1.0 M TRIS hydrochloride pH 8.6
$176.00
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HR2-941-34
StockOptions pH 34 185ml
1.0 M TRIS hydrochloride pH 8.8
$176.00
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HR2-941-35
StockOptions pH 35 185ml
0.5 M CAPSO pH 9.0
$231.00
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HR2-941-36
StockOptions pH 36 185ml
0.5 M CAPSO pH 9.2
$231.00
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HR2-941-37
StockOptions pH 37 185ml
0.5 M CAPSO pH 9.4
$231.00
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HR2-941-38
StockOptions pH 38 185ml
0.5 M CAPSO pH 9.6
$231.00
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HR2-941-39
StockOptions pH 39 185ml
0.5 M CAPSO pH 9.8
$231.00
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HR2-941-40
StockOptions pH 40 185ml
0.5 M CAPS pH 10.0
$266.00
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HR2-941-41
StockOptions pH 41 185ml
0.5 M CAPS pH 10.2
$266.00
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HR2-941-42
StockOptions pH 42 185ml
0.5 M CAPS pH 10.4
$266.00
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HR2-941-43
StockOptions pH 43 185ml
0.5 M CAPS pH 10.6
$266.00
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HR2-941-44
StockOptions pH 44 185ml
0.5 M CAPS pH 10.8
$266.00
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HR2-941-45
StockOptions pH 45 185ml
0.5 M CAPS pH 11.0
$266.00
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HR2-941-** Individual StockOptions pH Reagents SDS 
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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磷酸化lamban (D9W8M) 兔单克隆抗体
Phospholamban (D9W8M) Rabbit mAb
| 货号 | 产品名称 | 品牌 | 购买 |
|---|
| 货号 | 名称 | 单位 | 购买 |
|---|---|---|---|
| 14562S | 磷酸化lamban (D9W8M) 兔单克隆抗体 | 100µl | 咨询客服 |
|
品牌：FUJIFILM Wako
CAS No.： 储存条件：室温 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
233-56703 |
– | 1 column(W) | – | 咨询 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
| 货号 | 13815 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 200 Tests | 价格 | 3840 |
| Ex (nm) | 575 | Em (nm) | |
| 分子量 | 溶剂 | ||
| 产品详细介绍 | |||
简要概述
Amplite 比色法L-乳酸检测试剂盒是美国AAT Bioquest生产的用于检测乳酸的试剂盒，乳酸是手性的并且具有两种光学异构体：L-乳酸和D-乳酸。在代谢和运动过程中，乳酸盐通过乳酸脱氢酶（LDH）不断从丙酮酸产生。 监测乳酸水平是评估组织需氧量和利用率之间平衡的好方法，在研究细胞和动物生理学时非常有用。D-乳酸不被哺乳动物代谢，其从体内消除主要取决于肾脏排泄。D-和L-乳酸存在于许多发酵乳制品中，例如酸奶和奶酪，以及腌制蔬菜，腌制肉类和鱼类。D-和L-乳酸含量是食品的质量指标，如鸡蛋，牛奶，果汁和葡萄酒。血液中异常高浓度的D-乳酸通常是胃肠道中细菌过度生长的反映。
AAT Bioquest的Amplite 乳酸盐检测试剂盒（L＃乳酸检测Cat＃13814和13815，D-lactate检测Cat＃13810和13811）提供荧光和吸光度检测方法，用于检测L-乳酸盐或D-乳酸盐。生物样品，如血清，血浆，尿液，以及细胞培养样品。在酶偶联测定中，乳酸与NADH成比例相关，其通过生色NADH传感器特异性监测。通过吸光度酶标仪在~575nm或~55075nm / A605nm的吸光度比下可以容易地读取信号，以提高测定灵敏度。使用此Colorimetric Amplite L-乳酸测定试剂盒，我们能够在100μL反应体积中检测到低至4μM的L-乳酸。它应用广泛，可以很容易地适用于需要测量L-乳酸的各种应用。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Amplite 比色法L-乳酸检测试剂盒。
适用仪器
| 光吸收酶标仪 | |
| 吸收： | 570/605nm |
| 推荐孔板： | 透明底板 |
产品说明书
96孔板测定示例
概述
制备L-乳酸测定混合物（50μL）
加入L-乳酸标准品或测试样品（50μL）
在室温下孵育30分钟~2小时
监测吸光度比率在A575nm / A605nm处增加
注意：在开始实验之前，在室温下解冻每个试剂盒组分中的一个。
操作方法
1.准备NAD储备溶液（100X）：
向NAD小瓶（组分C）中加入100μLH2O，制成100×NAD储备溶液。
2.准备L-乳酸盐储备液：
将200μLH2 O或1×PBS缓冲液加入L-乳酸盐标准品（组分D）的小瓶中以制备100mM D-乳酸盐标准溶液。
注意：未使用的L-lacate标准储备溶液应分成单次使用的等分试样并储存在-20℃。
3.准备L-乳酸标准品（0至1 mM）的连续稀释液：
3.1将10μLL-乳酸盐储备液（来自步骤2）加入990L PBS缓冲液中，以产生1mM L-乳酸盐标准溶液。
注意：稀释的L-乳酸标准溶液不稳定，应在4小时内使用。
3.2取200μL的1mM L-乳酸标准溶液进行1：3连续稀释，得到L-Lactate标准品的300,100,30,10,3,1和0μM连续稀释液。
3.3如表1和2中所述，将L-乳酸盐标准品和含L-乳酸盐的测试样品的系列稀释液加入白色透明底96孔微孔板中。
4.制备L-乳酸测定混合物：
4.1将5 mL测定缓冲液（组分B）加入一瓶酶探针（组分A）中。
4.2将50μLNAD储备溶液（100X，来自步骤1）加入到组分A的瓶子中（来自步骤4.1），并充分混合。
注意：这种L-乳酸盐测定混合物足以用于一个96孔板。 未使用的L-乳酸盐测定混合物应分成单次使用的等分试样并储存在-20℃。
5.运行L-乳酸测定：
5.1将50μLL-乳酸盐测定混合物（来自步骤4.2）添加至L-乳酸盐标准品，空白对照和测试样品（参见步骤3.3）的每个孔中以使得总L-乳酸盐测定体积为100μL/孔。
注意：对于384孔板，在每个孔中加入25μL样品和25μLL-乳酸盐测定混合物。
5.2在室温下孵育反应30分钟至2小时，避光。
5.3用A575nm / A605nm的吸光度读板仪检测吸光度比率的增加。
参考文献
Exploring the Probiotic and Compound Feed Fermentative Applications of Lactobacillus plantarum SK1305 Isolated from Korean Green Chili Pickled Pepper
Authors: Kai-Min Niu, Damini Kothari, Sang-Buem Cho, Sung-Gu Han, In-Geun Song, Sam-Churl Kim, Soo-Ki Kim
Journal: Probiotics and Antimicrobial Proteins (2018): 1–12
Hypoxia induces lactate secretion and glycolytic efflux by downregulating mitochondrial pyruvate carrier levels in human umbilical vein endothelial cells
Authors: Dong Wang, Qingjie Wang, Gaoliang Yan, Yong Qiao, Boqian Zhu, Bo Liu, Chengchun Tang
Journal: Molecular medicine reports (2018)
Arenobufagin inhibits prostate cancer epithelial-mesenchymal transition and metastasis by down-regulating β-catenin
Authors: Liping Chen, Weiqian Mai, Minfeng Chen, Jianyang Hu, Zhenjian Zhuo, Xueping Lei, Lijuan Deng, Junshan Liu, Nan Yao, Maohua Huang
Journal: Pharmacological Research (2017)
Fibroblast Activation Protein Alpha-Activated Tripeptide Bufadienolide Anti-tumor Prodrug with Reduced Cardiotoxicity
Authors: Li-Juan Deng, Long-Hai Wang, Cheng-Kang Peng, Yi-Bin Li, Mao-Hua Huang, Min-Feng Chen, Xue-Ping Lei, Ming Qi, Yun Cen, Wen-Cai Ye
Journal: Journal of Medicinal Chemistry (2017)
Neuronal Culture Microenvironments Determine Preferences in Bioenergetic Pathway Use
Authors: Juliane Sünwoldt, Bert Bosche, Andreas Meisel, Philipp Mergenthaler
Journal: Frontiers in Molecular Neuroscience (2017): 305
The use of KnockOut serum replacement (KSR) in three dimentional rat testicular cells co-culture model: An improved male reproductive toxicity testing system
Authors: Xiaofang Zhang, Lei Wang, Xiaodong Zhang, Lijun Ren, Wenjing Shi, Yijun Tian, Jiangbo Zhu, Tianbao Zhang
Journal: Food and Chemical Toxicology (2017)
High glucose and interleukin 1β-induced apoptosis in human umbilical vein endothelial cells involves in down-regulation of monocarboxylate transporter 4
Authors: Dong Wang, Qingjie Wang, Gaoliang Yan, Yong Qiao, Ling Sun, Boqian Zhu, Chengchun Tang, Yuchun Gu
Journal: Biochemical and biophysical research communications (2015): 607–614
Intracellular ATP decrease mediates NLRP3 inflammasome activation upon nigericin and crystal stimulation
Authors: Johji Nomura, Alexander So, Mizuho Tamura, Nathalie Busso
Journal: The Journal of Immunology (2015): 5718–5724
相关产品
| 产品名称 | 货号 |
| Amplite 比色法D-乳酸检测试剂盒 | Cat#13811 |
| Amplite 比色法L-丙氨酸检测试剂盒 | Cat#13826 |
| Amplite 荧光法L-乳酸检测试剂盒 | Cat#13814 |
truXTRAC 血液cfDNA提取试剂盒（磁珠法）
truXTRAC cfDNA Kit – Magnetic Bead
产品概述：
Covaris truXTRAC® cfDNA提取试剂盒配合Covaris自动聚焦超声技术（Adaptive Focused Acoustics,AFA) ，相比传统方法，主动解交联提取cfDNA，cfDNA提取的得率、质量和复杂度都得到显著提高，更少的血液量即可满足下游检测分析需求，获取更多疾病相关信息。
truXTRAC® cfDNA提取试剂盒配合AFA技术主动提取：
ü主动解交联组蛋白、蛋白质、细胞外碎片与cfDNA的共价交联复合物，显著提高cfDNA产量和质量，相较传统方法得率提高70%；
ü获得信息更丰富，文库复杂度更高，除提取单核小体凋亡cfDNA外，还可获取血液中少量存在的核小体二聚体、三聚体cfDNA和非凋亡长片段cfDNA；
ü1-4ml，更少的血液量即可满足下游应用需求；
ü无需Carrier RNA，避免对下游检测的干扰；
ü室温操作，无需真空抽滤，仅需45min即可完成；
ü标准化流程，非接触，无污染，重复性高，易于高通量拓展。
技术指标
适用平台：Covaris 平台；
适用样本：血液，样品1-4ml
说明：应用Covaris自动聚焦声学 AFA（Adaptive Focused Acoustics™）技术，需配套Covaris 仪器使用，试剂盒已包含AFA管子。
| 货号 | 产品名称 | 品牌 | 购买 |
|---|
| 货号 | 名称 | 单位 | 购买 |
|---|---|---|---|
| 520221 | truXTRAC 血液cfDNA提取试剂盒（磁珠法） | 24次 | 咨询客服 |
|
品牌：FUJIFILM Wako
CAS No.： 储存条件：室温 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
234-55513 |
– | 1 column(W) | – | 咨询 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Salts > Potassium acetate
Potassium acetate
Synonyms: K(acac)
C2H3KO2
CH3COOK
Mr 98.14
CAS Number [127-08-2]
EC Number 204-822-2
Merck 14,7605
RTECS AJ3325000
MDL Number MFCD00012458
Beilstein Registry Number 3595449
Purity ≥ 99.0%
Measured pH range: 7.9 – 9.9 at 25°C
Measured Conductivity Range: 132.3 – 151.2 mS/cm at 25°C
Measured Refractive Index Range: 1.38123 – 1.38216 at 20°C
Insoluble Matter ≤0.005%
Ca ≤0.005%
Cl ≤0.003%
Fe ≤5 ppm (parts per million)
Mg ≤0.002%
Na ≤0.03%
Pb ≤5 ppm (parts per million)
PO4 ≤0.001%
SO4 ≤0.002%
Click to Zoom In
HR2-671
5.0 M Potassium acetate
200 mL
$107.00
cart quote
HR2-671 Potassium acetate SDS 
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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| Website by Skyhound Internet
CutSmart Buffer: 100%
CutSmart、重组酶、省时酶。
10,000 units/ml。
lumiprobe荧光染料官网代理商 水溶性荧光染料代理商 脂溶性荧光染料代理商
| Cat. # | Quantity | Price | Lead time | Buy this product | ||
|---|---|---|---|---|---|---|
| 1D420 | 1 mg | 110.00$
|
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| 2D420 | 5 mg | 210.00$
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| 4D420 | 25 mg | 410.00$
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| 5D420 | 50 mg | 695.00$
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| 6D420 | 100 mg | 1190.00$
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BDP TR is a borondipyrromethene dye which has absorption and emission wavelengths close to ROX dye. The fluorophore is bright, moderately hydrophobic, and has low molecular weight. An extra linker based on aminocaproic acid (C6) provide better separation between the dye, and the biomolecule.
The NHS ester function can be used for the conjugation with proteins, peptides, and other molecules possessing amine group.
Download in
ChemDraw format
Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
磷酸化EGF 受体 (Tyr1068) (D7A5) XP® 兔单克隆抗体 (PE 偶联)
Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb (PE Conjugate)
| 货号 | 产品名称 | 品牌 | 购买 |
|---|
| 货号 | 名称 | 单位 | 购买 |
|---|---|---|---|
| 14565S | 磷酸化EGF 受体 (Tyr1068) (D7A5) XP® 兔单克隆抗体 (PE 偶联) | 100µl | 咨询客服 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
| 货号 | 13820 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 200 Tests | 价格 | 3840 |
| Ex (nm) | 571 | Em (nm) | 585 |
| 分子量 | 溶剂 | ||
| 产品详细介绍 | |||
简要概述
Amplite 荧光法丙酮酸检测试剂盒是美国AAT Bioquest生产的用于检测丙酮酸的试剂盒，丙酮酸是细胞内代谢途径中的重要化合物。它来源于称为糖酵解的葡萄糖代谢。一分子葡萄糖分解成两个丙酮酸分子，通过两种方式之一提供活细胞能量。当氧气存在时（有氧呼吸），丙酮酸被丙酮酸脱氢酶转化为乙酰-CoA，丙酮酸脱氢酶进入柠檬酸循环（也称为三羧酸循环）产生ATP。当氧气不足时，丙酮酸盐会厌氧分解，在动物体内产生乳酸盐，在植物和微生物体内产生乙醇。丙酮酸盐的异常水平或乳酸盐与丙酮酸盐的浓度比可能与肝脏疾病或代谢紊乱有关，并且是患者临床和其他实验室研究中的一种诊断测量。 AAT Bioquest的Amplite 比色丙酮酸测定试剂盒提供了一种灵敏的比色测定法，用于定量样品中的丙酮酸。它利用释放过氧化氢的酶偶联反应，可通过吸光度酶标仪在575 nm处通过丙酮酸盐传感器检测。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Amplite 荧光法丙酮酸检测试剂盒。
适用仪器
| 荧光酶标仪 | |
| 激发： | 540nm |
| 发射： | 590nm |
| cutoff： | 570nm |
| 推荐孔板： | 黑色孔板 |
产品说明书
样品实验方案
简要概述
1.准备测试样品（50 µL）以及连续稀释的丙酮酸标准液（50 µL）
2.加入等体积的丙酮酸工作溶液（50 µL）
3.在室温下孵育30分钟至1小时
4.监测Ex / Em = 540/590 nm的荧光强度
溶液制备
1.储备溶液
所有未使用的储备溶液应分为一次性使用的等分试样，并在制备后储存在-20°C下。 避免重复冻融循环。
1.1 Quest Fluor 丙酮酸传感器储备溶液（200X）：
将55 µL DMSO（组分E）添加到Quest Fluor 丙酮酸传感器（组分A）中，制成200X Quest Fluor 丙酮酸传感器储备溶液。
1.2 丙酮酸标准溶液（1 mM）：
将10 µL 100 mM丙酮酸盐（组分D）添加到990 µL PBS（pH 7）中，得到1 mM丙酮酸盐溶液。
2.标准溶液
丙酮酸标准
将100 µL的1 mM丙酮酸标准溶液加入900 µL PBS中制成100 µM丙酮酸溶液（PS7）。 然后执行1：3连续稀释以获得连续稀释的丙酮酸标准品（PS6-PS1）。
3.工作溶液
3.1 将5 mL测定缓冲液（组分C）加入一瓶酶混合1瓶（组分B1）中； 混合均匀。
3.2 将100μLddH2O加入一个酶混合物2小瓶（组分B2）中； 混合均匀。
3.3 将整个小瓶（100μL）的Enzyme Mix 2和25 uL的200X Quest Fluor 丙酮酸传感器储备溶液转移到Enzyme Mix 1瓶中。 混合均匀。 注意：丙酮酸工作溶液不稳定，应立即使用，避免光照。
样品操作及实验分析
表1.黑色96孔微孔板中丙酮酸标准品和测试样品的布局。 PS =丙酮酸标准溶液（PS1-PS7，0.1至100 µM）； BL =空白对照； TS =测试样品。
| BL | BL | TS | TS |
| PS1 | PS1 | … | … |
| PS2 | PS2 | … | … |
| PS3 | PS3 | ||
| PS4 | PS4 | ||
| PS5 | PS5 | ||
| PS6 | PS6 | ||
| PS7 | PS7 |
表2.每个孔的试剂组成
| 孔 | 容积 | 试剂 |
| PS1-PS7 | 50ul | 连续稀释（0.1至100 µM） |
| BL | 50ul | PBS |
| TS | 50ul | 样品 |
1.根据表1和表2提供的布局，将丙酮酸标准液（PS），空白对照（BL）和测试样品（TS）制备成黑色的96孔微孔板。对于384孔板，请使用25 µL 每孔试剂，而不是50 µL。
2.在丙酮标准液，空白对照和测试样品的每个孔中加入50 µL丙酮酸工作溶液，使丙酮酸测定总体积为100 µL /孔。 对于384孔板，请向每个孔中添加25 µL工作溶液，总体积为50 µL /孔。 在6.5至7.0的pH值下运行丙酮酸盐测定。
3.将反应混合物在室温下孵育30分钟至1小时。
4.使用荧光板读数器在Ex / Em = 540/590 nm（截止：570 nm）下监测荧光的增加。
参考文献
A replaceable dual-enzyme capillary microreactor using magnetic beads and its application for simultaneous detection of acetaldehyde and pyruvate
Authors: Shi J, Zhao W, Chen Y, Guo L, Yang L.
Journal: Electrophoresis (2012): 2145
Detection of inflammatory arthritis by using hyperpolarized 13C-pyruvate with MR imaging and spectroscopy
Authors: MacKenzie JD, Yen YF, Mayer D, Tropp JS, Hurd RE, Spielman DM.
Journal: Radiology (2011): 414
Amplex UltraRed enhances the sensitivity of fluorimetric pyruvate detection
Authors: Zhu A, Romero R, Petty HR.
Journal: Anal Biochem (2010): 123
Comparison of high-performance liquid chromatography with fluorescence detection versus enzymatic assay to measure blood pyruvate in clinical practice
Authors: Wolff F, El Khattabi C, Bourdon F, Willems D.
Journal: Clin Biochem (2009): 1099
Faecal tumour pyruvate kinase M2: not a good marker for the detection of colorectal adenomas
Authors: Shastri YM, Stein JM.
Journal: Br J Cancer (2008): 1366; author reply 1367
Hyperpolarized 13C lactate, pyruvate, and alanine: noninvasive biomarkers for prostate cancer detection and grading
Authors: Albers MJ, Bok R, Chen AP, Cunningham CH, Zierhut ML, Zhang VY, Kohler SJ, Tropp J, Hurd RE, Yen YF, Nelson SJ, Vigneron DB, Kurhanewicz J.
Journal: Cancer Res (2008): 8607
Sensitivity and specificity of faecal tumour M2 pyruvate kinase for detection of colorectal adenomas in a large screening study
Authors: Haug U, Hundt S, Brenner H.
Journal: Br J Cancer (2008): 133
Detection of pyruvate dehydrogenase E1 alpha-subunit deficiencies in females by immunohistochemical demonstration of mosaicism in cultured fibroblasts
Authors: Lib MY, Brown RM, Brown GK, Marusich MF, Capaldi RA.
Journal: J Histochem Cytochem (2002): 877
Tumor M2-pyruvate kinase in lung cancer patients: immunohistochemical detection and disease monitoring
Authors: Schneider J, Neu K, Grimm H, Velcovsky HG, Weisse G, Eigenbrodt E.
Journal: Anticancer Res (2002): 311
Measurement of pyruvate in blood by high-performance liquid chromatography with fluorescence detection
Authors: Wulkan RW, Verwers R, Neele M, Mantel MJ.
Journal: Ann Clin Biochem (2001): 554
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Hampton Research蛋白结晶试剂盒
Products > Custom Shop Crystallization Reagents > StockOptions PIPES > Individual StockOptions PIPES Reagents
HR2-257-**
** = Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-257-01
PIPES titrated to indicated pH using NaOH
PIPES
Synonyms: 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)
C8H18N2O6S2
Mr 302.37
CAS Number [5625-37-6]
EC Number 227-057-6
Beilstein Registry 817713
Purity ≥ 99%
pKa 6.8
Useful pH range of PIPES buffer 6.1-7.5
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HR2-257-01
StockOptions PIPES 01 185ml
1.0 M PIPES pH 6.1
$198.00
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HR2-257-02
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HR2-257-** Individual StockOptions PIPES Reagents SDS 1. The expression, purification and crystallization of the epsilon subunit of the F1 portion of the ATPase of Escherichia coli. Codd, R; Cox, GB; Guss, JM; Solomon, RG; Webb, D. J Mol Biol 228, 306- 309, 1992.
2. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
3. Microscale Fluorescent Thermal Stability Assay for Membrane Proteins Structure, Volume 16, Issue 3, 11 March 2008, Pages 351-359 Alexander I. Alexandrov, Mauro Mileni, Ellen Y.T. Chien, Michael A. Hanson, Raymond C. Stevens
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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| Website by Skyhound Internet
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品牌：FUJIFILM Wako
CAS No.： 储存条件：室温 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
238-51611 |
– | 1 column(D) | – | 咨询 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Salts > Potassium bromide
Potassium bromide
Synonym: None
KBr Mr 119.00
CAS Number [7758-02-3]
EC Number 231-830-3
Merck 14,7618
RTECS TS7650000
MDL Number MFCD00011358
PubChem Substance ID 24854613
Purity ≥99.0%
Measured pH range: 5.6 – 9.1 at 25°C
Measured Conductivity Range: 430.8 – 440.4 mS/cm at 25°C
Measured Refractive Index Range: 1.38427 – 1.38495 at 20°C
Total Impurities ≤0.005% (insolubles)
Ba ≤0.002%
BrO3 ≤0.001%
Ca ≤0.002%
Cl ≤0.2%
Fe ≤5 ppm
IO3 ≤0.001%
I ≤0.001%
Mg ≤0.001%
Na ≤0.02%
SO4 ≤0.005%
Heavy metals (as Pb) ≤5 ppm
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HR2-779
4.0 M Potassium bromide
100 mL
$92.00
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HR2-779 Potassium bromide SDS 1. Isolation of Ferritin Crystal from Rabbit Liver and Spleen. NORIKO MURAOKA, SHINJI NAKAJIMA, TAKESHI KATO, and TOSHIO SHIMADA. The Journal of Biochemistry Volume 60, Number 5 Pp. 489-495 (1966).
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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truXTRAC FFPE DNA和RNA提取试剂盒（磁珠纯化）-130ul小提
truXTRAC FFPE total NA Kit – Magnetic Bead
| 货号 | 产品名称 | 品牌 | 购买 |
|---|
| 货号 | 名称 | 单位 | 购买 |
|---|---|---|---|
| 520246 | truXTRAC FFPE DNA和RNA提取试剂盒（磁珠纯化）-130ul小提 | 25次/ kit | 咨询客服 |
NEBuffer 1.1: <10%
NEBuffer 2.1: <10%
NEBuffer 3.1: 100%
CutSmart Buffer: <10%
重组酶、省时酶。
NEBuffer 3.1，37℃。
热失活：65℃ 20 分钟。
20,000 和 100,000 units/ml。
对哺乳动物基因组 DNA CpG 甲基化敏感。
当切割超螺旋形式的 pBR322 和 pUC质粒时，完全消化需要 10 倍量的 Sall 酶。
|
品牌：FUJIFILM Wako
CAS No.： 储存条件：室温 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
237-01511 |
– | 3EA | – | 咨询 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。
