For the measurement of endo-1,4-?-D-xylanase in enzyme preparations,bread improver mixtures and animal feeds. Contains Xylazyme AX Tabletsand xylanase enzyme controls (A. niger and Trichoderma longibrachiatum).
INTRODUCTION:
Arabinoxylan is the major endosperm cell-wall polysaccharide of
wheat and rye and is found in significant proportions in most cereal
solutions and slurries of high viscosity, and in animal nutrition it
reduces the rate of nutrient absorption from the gut.
endo-Beta-D-Xylanase (xylanase) is added to feeds to catalyse
depolymerisation of this polysaccharide. It can be demonstrated
that endo-cleavage by xylanase of just one bond per thousand in the
arabinoxylan backbone can significantly remove viscosity properties.
Of the carbohydrase enzymes used as feed supplements, one of the
most difficult to measure has been xylanase. These problems are
attributed to several factors, including the low levels of enzyme
added to the feed, inactivation of enzyme during pelleting, binding
of the enzyme to feed components and the presence of specific
xylanase inhibitors.
The only biochemical methods which are sufficiently sensitive, specific
and robust to measure xylanase in feeds are viscometric assays and
those employing dyed xylan or arabinoxylan polysaccharides.
Viscometric assays are tedious, whereas assays employing dyed
xylan substrates are rapid, reproducible and simple to perform.
We recommend the use of either Xylazyme AX tablets or Azo-
Wheat Arabinoxylan (Azo-WAX). Xylazyme AX based assays
are about 5-fold more sensitive than assays employing Azo-WAX.
However, this latter substrate does have sufficient sensitivity in most
applications, and results are slightly more reproducible than with
Xylazyme AX.
It is generally accepted that xylanase enzymes which are best suited
to feed applications have optimal activity at pH 6.0. Consequently,
these enzymes are generally assayed at this pH in 100 mM sodium
phosphate buffer. In recovery experiments, however, we found that
sodium phosphate buffer extracts only a small proportion (< 20%)
of the amount of enzyme added to the feed. Thus a wide range of
alternative extractants and extraction conditions have been evaluated.
For feeds containing Trichoderma sp. xylanases, the best and most
consistent results have been obtained using 100 mM acetic acid
or 100 mM sodium acetate buffer (pH 4.7) at room temperature.
Optimal extraction of Humicola sp. xylanases was achieved with a
buffer containing 100 mM MES buffer (pH 6.0) and 1 % w/v sodium
dodecyl sulphate (SDS).
KITS:
Kits containing the required reagents to measure xylanase in animal
feeds are available from Megazyme. These kits contain:
1. Xylazyme AX test tablets (200 tablets).
2. A. niger control xylanase (~ 295 mU/mL at 40°C and pH 4.7)
in 50 % (v/v) glycerol (activity stated on vial).
3. T. longibrachiatum control xylanase (~ 386 mU/mL at 40°C and
pH 6.0) in 50% (v/v) glycerol (activity stated on vial).
爱尔兰Megazyme公司是全球知名的酶及酶法分析试剂盒的生产厂家，
其中大多数产品都被AOAC(Association of Official Analytical Chemists)、
AACC(American Association of Cereal Chemists)、ICC(InternationalAssociation for Cereal Science and Technology)、
EBC(European Brewing Convention)、RACI(Royal Australian Chemical Institute)等国际组织采用。
Xylazyme AX Tablets have been widely adopted in the fermentation andfeeds industries for the measurement of Xylanase.
Beta-葡聚糖检测盒Beta-Glucan (Mixed Linkage) Assay Kit
AACC Method 32-23
EBC Methods 3.11.1, 4.16.1 and 8.11.1
AOAC Method 995.16
ICC Standard No. 166
RACI Standard Method
极限糊精酶和支链淀FEN酶检测片剂（Limit-Dextrizyme）
RACI Standard Method
Beta-葡聚糖酶检测盒（Beta-Glucanase (Malt and Microbial) Assay Kit）
RACI Standard Method
Beta-淀FEN酶检测盒（Betamyl (Beta-Amylase Assay Kit)）
RACI Standard Method.
木聚糖酶检测底物（Azo-Wheat Arabinoxylan ）
Azo-Wheat Arabinoxylan, Azo-CM-Cellulose and Ceralpha methods for theassay of Xylanase, cellulase and ?-Amylase, respectively, have beenadopted by the UK silage industry.
(3) Dietary fiber determined gravimetrically following alcohol
precipitation
(4) Ash and residual protein determined on DF residues
and subtracted
Kit size: 100 / 200 assays Method: Hydrolysis / removal of non-dietary
fibre components Total assay time: ~ 100 min Detection limit: 0.5-100% of sample weight Application examples: Food ingredients, food products and other materials Method recognition: AOAC (Methods 985.29, 991.42, 991.43 and 993.19), AACC
(Methods 32-05.01, 32-06.01, 32-07.01 and 32-21.01) and
CODEX (Type I Method)
Total Dietary Fiber Assay Kit, for the measurement and analysis of total, soluble and insoluble dietary fiber according to AOAC and AACC approved methods. See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.
Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our booklet are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.
1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.
2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.
3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.
Two separate methods are described in the associated data booklet:
METHOD 1:
DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER
Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).
METHOD 2:
DETERMINATION OF TOTAL DIETARY FIBER
Based on AACC method 32-05.01 and AOAC Method 985.29.
Advantages
Very competitive price (cost per test)
All reagents stable for > 2 years
High purity / standardised enzymes employed
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
详细描述： Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb 兔单抗能检测内源性Thr183和Tyr185磷酸化的p46 、p54 SAPK/JNK蛋白水平。该抗体可能与磷酸化的p44/42或p38 MAPK有交叉反应。该单克隆抗体是采用合成的与人源SAPK/JNK蛋白Thr183/Tyr185周围残基相一致的磷酸化肽段免疫动物而获得。Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb detects endogenous levels of p46 and p54 SAPK/JNK only when phosphorylated at Thr183 and Tyr185. This antibody may cross-react with phosphorylated p44/42 or p38 MAP kinases.Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK.
