详细描述： The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as prolifer
Effectene Transfection Reagent is an innovative non-liposomal lipid formulation that is used in conjunction with a special DNA-condensing enhancer and optimized buffer to achieve high transfection efficiencies. The enhancer first condenses the DNA molecules and Effectene Reagent subsequently coats them with cationic lipids （see figure “Model of Effectene Principle”）, providing a particularly efficient way of transferring DNA into eukaryotic cells. Effectene Reagent offers significant advantages over many liposome reagents and other transfection methods （1, 2）.
Procedure
The Effectene procedure has two steps. DNA is first mixed with Enhancer and a buffer that provides optimal salt conditions for efficient DNA condensation. This step requires just 2–5 minutes. Effectene Reagent is then added and the mixture is incubated for 5–10 minutes to allow Effectene–DNA complexes to form. The complexes are mixed with growth medium （which can contain serum and antibiotics）, and added directly to the cells. The cells are then incubated until harvested and analyzed for gene expression （see flowchart “Effectene Transfection Reagent”）.
Applications
Effectene Reagent is suitable for transient and stable transfection of a broad range of cell types （see figures “High Transfection Efficiencies Using Effectene Reagent” and “Transfection of Oligonucleotides Using Effectene Reagent”）. Because transfection can be performed in the presence of serum and requires low amounts of DNA （see figure “Serum and DNA Quantity vs. Transfection Efficiency”）, cytotoxicity is minimal. This makes Effectene Reagent particularly effective for primary cells （see figure “Effectene Reagent with Primary Cells”）. A searchable list of cell lines and primary cells successfully transfected using Effectene Reagent, as well as customer-developed transfection protocols, is available at the Transfection Tools web site — www.qiagen.com/TransfectionTools. View cell lines successfully transfected with this product.
Effectene Transfection Reagent effectene转染试剂
High-throughput transfection
The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using Effectene Reagent requires low amounts of DNA and minimal handling, and in addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. Effectene Reagent provides outstanding transfection efficiencies, excellent reproducibility, and minimal cytotoxicity in high-throughput transfection, and is available in bulk quantities.
Cited References
1. Ausubel, F.M., et al. eds. （1991） Current Protocols in Molecular Biology, Wiley Interscience, New York. 2. Sambrook, J., Fritsch, E.F., and Maniatis, T., eds. （1989） Molecular cloning — a laboratory manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
Images
Model of Effectene Principle High Transfection Efficiencies Using Effectene Reagent Transfection of Oligonucleotides Using Effectene Reagent Effectene Transfection Procedure Serum and DNA Quantity vs. Transfection Efficiency Effectene Reagent with Primary Cells
*Reconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer （e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0） or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM. The sugar solution can be diluted further when appropriate or the sugar may be reconstituted with a smaller volume of water or buffer, if the sugar is soluble, to increase the final concentration. This sugar is suitable for diluting labeled lectins to demonstrate lectin specificity by inhibiting binding or for eluting glycoconjugates from agarose-bound lectins.
Model of Effectene Principle 
High Transfection Efficiencies Using Effectene Reagent 
Transfection of Oligonucleotides Using Effectene Reagent 
Effectene Transfection Procedure 
Serum and DNA Quantity vs. Transfection Efficiency 
Effectene Reagent with Primary Cells
