BD溶血素FACS Lysing Solution红细胞裂解液

1) 全血制备时用于溶解红细胞
(2) 固定外表位
(3) 辅助通透剂

BD溶血素FACS Lysing Solution红细胞裂解液

 FACS溶血素使用前用无离子水1：10稀释，勿用PBS或其它缓冲液。而且FACS溶血素必须与FACS通透液一起使用，即使是培养细胞的检测。

BD FACS™ lysing solution is intended for lysing red blood cells following direct immunofluorescence staining of human peripheral blood cells with monoclonal antibodies prior to flow cytometric analysis.

BD FACS lysing solution is appropriate for use with reagents such as BD Tritest™ or BD Simultest™ reagents and a suitably equipped flow cytometer. It may be used in both lyse/wash and lyse/no-wash procedures.

BD溶血素/流逝细胞分析用溶血素100ml，BD溶血素/流逝细胞分析用溶血素100ml，

D FACS lysing solution (10X) is stable until the expiration date shown on the bottle label when stored as directed. Do not use this reagent if discoloration occurs or a precipitate forms.

FACS溶血素（BDIS Catalog No.349202）用于PBMCs，培养细胞与全血制备，主要有3个作用： 
(1) 全血制备时用于溶解红细胞
(2) 固定外表位
(3) 辅助通透剂
     FACS溶血素使用前用无离子水1：10稀释，勿用PBS或其它缓冲液。而且FACS溶血素必须与FACS通透液一起使用，即使是培养细胞的检测。

BD FACS™ lysing solution is intended for lysing red blood cells following direct immunofluorescence staining of human peripheral blood cells with monoclonal antibodies prior to flow cytometric analysis.

BD FACS lysing solution is appropriate for use with reagents such as BD Tritest™ or BD Simultest™ reagents and a suitably equipped flow cytometer. It may be used in both lyse/wash and lyse/no-wash procedures.

miltenyi 美天旎MACS Tissue Storage Solution

miltenyi 美天旎MACS Tissue Storage Solution

• Convenient: store tissues up to 48 hours
• Efficient: no background effects like cell activation or apoptosis
• Flexible: tested for variety of tissue types

The MACS® Tissue Storage Solution has been developed to allow an optimized storage of fresh organ and tissue samples without showing background effects like cell activation or apoptosis induction. It has been tested and validated on a variety of human and murine tissues including tumors, skin, heart, spleen, brain, and skeletal muscle.

miltenyi美天旎Human IL-2 IS细胞因子，miltenyi美天旎Human IL-2 IS细胞因子，

IL-2 IS stands for interleukin 2 “Improved Sequence”, also termed aldesleukin, and is a variant of IL-2 with a serine to cysteine substitution. Human IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays, and possesses the same properties as naturally occurring IL-2.

Background information

IL-2, a potent lymphoid cell growth factor, is a typical four α-helix bundle cytokine. It is produced by activated T cells, especially the CD4+ T helper cell population. It plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes proliferation and differentiation of T cells, NK cells and B cells and is involved in the elimination of self-reactive T cells. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β- chain, and common γ-chain. The latter two are also used for IL-15 signaling.

Biological activity

Proliferation of CTLL-2 cells (NIBSC 86/504)

research grade: ≥ 3×106 IU/mg

premium grade: ≥ 5×106 IU/mg

Quality description

Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

miltenyi 美天旎磁珠CD25 MicroBeads II, human  – lyophilized

品牌：    Miltenyi(美天旎)
货号：    130-097-044
CAS号：    详见说明书
供应商：    Miltenyi(美天旎)
保质期：    详见说明书
保存条件：    详见说明书
规格：    EA

miltenyi 美天旎磁珠CD25 MicroBeads II, human  – lyophilized

CD25 MicroBeads II were developed for positive selection or depletion of CD25+ cells.

miltenyi 美天旎磁珠CD25 MicroBeads II, human  – lyophilized

Background information

CD25, the low-affinity interleukin-2 receptor alpha chain (IL-2Rα), is expressed on activated T and B cells and monocytes. CD25 is also present on a subset of non-activated CD4+ T cells, which has regulatory function.

The CD25 MicroBeads II recognize epitope A of the CD25 molecule.

For control staining of cells separated with CD25 MicroBeads II, it is recommended to use MACS® CD25 Antibodies, which bind to epitope B of the CD25 antigen.

For isolation of CD25+ cell subsets, the CD25 MicroBeads II can be combined with MACS® Cell Isolation Kits or MultiSort Kits. The CD4+CD25+ Regulatory T Cell Isolation Kit is recommended for most efficient separation of CD4+CD25+ regulatory T cells.

Columns

For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.

miltenyi美天旎human IL-15蛋白,人白介15细胞生长因子，miltenyi美天旎human IL-15蛋白,人白介15细胞生长因子，

igure 1

Human IL-15 activity assay. The biological activity of Human IL-15 is determined by proliferation assay using CTLL2 cells.

Figure 2

SDS-PAGE of Human IL-15, premium grade under reduced (R) and non-reduced (NR) conditions.

Figure 3

Mass spectrometry analysis (ESI-MS) of Human IL-15, premium grade. The peak corresponds to the calculated molecular mass of 1277

订购货号：130-095-765

THERMO超敏发光液34095（化学发光ECL底物）

描述

Thermo Scientific SuperSignal West Femto zui高灵敏度底物是一款具有超高灵敏度的增强型化学发光(ECL)底物，可通过辣根过氧化物酶(HRP)实现低飞克级的免疫印迹检测。

THERMO超敏发光液34095（化学发光ECL底物）

SuperSignal West Femtozui高灵敏度底物的特点：

• ECL —用于辣根过氧化物酶(HRP)的增强型化学发光底物
• 灵敏—使用适当的一抗和二抗时，可在硝化纤维膜或PVDF膜上检测丰度为低飞克（中zeptomole（10^21）)级的蛋白条带
• 定量—所得信号的可定量检测范围跨越两个数量级
• 明亮信号—通过胶片或成像系统进行曝光，易于捕获图像
• 长信号持续时间—优化条件下，可检测的光信号输出长达8小时
• 稳定试剂—试剂盒组分能够在4°C条件下稳定放置一年，在室温下可稳定放置6个月
• 价格经济— 配方经过优化，可适用于浓度极低的抗体检测
• 1ng至0.2 µg/mL一抗（以1 µg/mL储存液稀释1:5,000至1:100,000倍）
• 2ng至10 ng/mL二抗（以1 µg/mL储存液稀释1:100,000至1:500,000倍）

当SuperSignal West Femto底物与优化的抗体浓度和封闭缓冲液配合使用时，可检测到常规ECL底物无法检测的低丰度靶标蛋白。

pierce超高敏化学发光底物SuperSignal West Femto，pierce超高敏化学发光底物SuperSignal West Femto，

SuperSignal West Femtozui高灵敏度底物的特点：

• ECL —用于辣根过氧化物酶(HRP)的增强型化学发光底物
• 灵敏—使用适当的一抗和二抗时，可在硝化纤维膜或PVDF膜上检测丰度为低飞克（中zeptomole（10^21）)级的蛋白条带
• 定量—所得信号的可定量检测范围跨越两个数量级
• 明亮信号—通过胶片或成像系统进行曝光，易于捕获图像
• 长信号持续时间—优化条件下，可检测的光信号输出长达8小时
• 稳定试剂—试剂盒组分能够在4°C条件下稳定放置一年，在室温下可稳定放置6个月
• 价格经济— 配方经过优化，可适用于浓度极低的抗体检测
• 1ng至0.2 µg/mL一抗（以1 µg/mL储存液稀释1:5,000至1:100,000倍）
• 2ng至10 ng/mL二抗（以1 µg/mL储存液稀释1:100,000至1:500,000倍）

当SuperSignal West Femto底物与优化的抗体浓度和封闭缓冲液配合使用时，可检测到常规ECL底物无法检测的低丰度靶标蛋白。

miltenyi美天旎CD34分选磁珠human Diamond CD34 Isolation Kit，miltenyi美天旎CD34分选磁珠human Diamond CD34 Isolation Kit，t

The Diamond CD34 Isolation Kit has been specifically developed for the isolation of highly pure Lin–CD34+ hematopoietic stem cells from peripheral blood1, bone marrow2,3 or cord blood.

订购信息：

miltenyi美天旎MACS Supplement B27 PLUS（NeuroBrew-21）

miltenyi美天旎MACS Supplement B27 PLUS（NeuroBrew-21）

MACS® NeuroBrew®-21 (formerly MACS Supplement B27 PLUS) are serum-free neural supplements designed for use in combination with MACS Neuro Medium for the in vitropropagation of mature neural cells of the central and peripheral nervous system, or neural stem cells of primary or ES/iPS cell origin.

MACS® NeuroBrew-21 is a re-optimized formulation of the B27 components designed to improve performance.1 It has been developed for the in vitro propagation of neural cells of the central and peripheral nervous system.

MACS NeuroBrew-21 has been designed for

• optimal growth and long term viability of neural cells of the central and peripheral nervous system
• the growth of nearly pure populations of neural cells without the need of an astrocyte feeder layer

MACS NeuroBrew-21 w/o Vitamin A is identical to MACS NeuroBrew-21, but without the addition of Vitamin A or other retinoid related components. Vitamin A induces the differentiation of neural stem cells into mature neural cells. MACS NeuroBrew-21 w/o Vitamin A is suitable for use with studies of neural stem cells, neural development and neural cell differentiation.

MACS NeuroBrew-21 w/o Vitamin A has been designed for use with

• primary neural stem or progenitor cells
• primary neurospheres
• ES/iPS cell derived neural stem cells and neurosphere

订购信息：

miltenyi美天旎Whole Blood Column Kit

The Whole Blood Column Kit is suited for the separation of leukocyte subsets directly from anticoagulated human whole blood or bone marrow. The kit contains Whole Blood Columns and Whole Blood Column Elution Buffer for convenient positive selection of cells labeled with Whole Blood MicroBeads.

Whole Blood Columns are designed for use with:

• MidiMACS™ Separator
• QuadroMACS™ Separator
• VarioMACS™ Separator, in combination with an LS Column Adapter
• SuperMACS™ II Separator
• MultiMACS Cell24 Separator Plus, in combination with the Single Column Adapter

miltenyi美天旎Whole Blood Column Kit

订购信息：

miltenyi美天旎磁珠分选gentleMACS 25 C Tubes

miltenyi美天旎磁珠分选gentleMACS 25 C Tubes

millipore悬挂式24孔板细胞小室PET（8 µm），millipore悬挂式24孔板细胞小室PET（8 µm），millipore悬挂式24孔板细胞小室PET（8 µm）

Polyethylene Terephthalate hanging cell culture insert with pore size of 8.0 µm used in a 24-well plate for cell attachment, cell culture, cell differentiation & ICC.

货号    描述（悬挂式）
PIHT12R48    Millicell Hanging Cell Culture 24 well PET 0.4um 48/pk
PIRP12R48    Millicell Hanging Cell Culture 24 well PET 1um 48/pk
PISP12R48    Millicell Hanging Cell Culture 24 well PET 3um 48/pk
PIMP12R48    Millicell Hanging Cell Culutre 24 well PET 5um 48/pk
PIEP12R48    Millicell Hanging Cell Culture 24 well PET 8um 48/pk
PIHT15R48    Millicell Hanging Cell Culutre 12 well PET 0.4um 48/pk
PIRP15R48    Millicell Hanging Cell Culture 12 well PET 1um 48/pk
PISP15R48    Millicell Hanging Cell Culture 12 well PET 3um 48/pk
PIMP15R48    Millicell Hanging Cell Culture 12 well PET 5um 48/pk
PIEP15R48    Millicell Hanging Cell Culture 12 well PET 8um 48/pk
PIHT30R48    Millicell Hanging Cell Culture 6 well PET 0.4um 48/pk
PIRP30R48    Millicell Hanging Cell Culture 6 well PET 1um 48/pk
PISP30R48    Millicell Hanging Cell Culture 6 well PET 3um 48/pk
PIMP30R48    Millicell Hanging Cell Culture 6 well PET 5um 48/pk
PIEP30R48    Millicell Hanging Cell Culture 6 well PET 8um 48/pk
      
货号    描述（插入式）
PICM03050    Milli Stand Insrt 6 well CM 0.4um 50/pk
PIHP03050    Milli Stand Insrt 6 well PCF 0.4um 50/pk
PIHA03050    Milli Stand Insrt 6 well HA 0.45um 50/pk
PIHP01250    Milli Stand Insrt 24 well PCF0.4um 50/pk
PITP01250    Milli Stand Insrt 24 well PCF 3um 50/pk
PI8P01250    Milli Stand Insrt 24well PCF 8.0um 50/pk
PIXP01250    Milli Stand Insrt 24 well PCF 12um 50/pk
PICM01250    Milli Stand Insrt 24 well CM 0.4um 50/pk
PIHA01250    Milli Stand Insrt 24 well HA 0.45um 50/pk
PICM0RG50    Milli Stand Insrt 0.4um Organotypic 50/pk

millipore MCEP24H48悬挂式细胞培养皿,millipore MCEP24H48悬挂式细胞培养皿,

millipore Millicell插入式细胞培养皿,Transwell细胞小室0.4um

描述
产品目录编号    PIHP01250
商名    
Millicell
描述    Millicell 插入式细胞培养皿, 12 mm, 聚碳酸酯, 0.4 µm
产品信息
Device Configuration    24-well plate
过滤器代码    PCF
zui高工作温度    50 °C
孔数    1

millipore Millicell插入式细胞培养皿,Transwell细胞小室0.4um

应用
应用    zui大的化合物运输和可重现药物吸收效果，经处理用于组织培养，不需要ECM*
主要应用    
Cell Attachment
Cell Culture
生物信息
媒质    Isopore®
无菌性    无菌
Stem Cell Type    
Mouse Embryonic Stem Cells
Human Embryonic Stem Cells
Mesenchymal Stem Cells
Neural Stem Cells
Hematopoietic Stem Cells
Epithelial Cells
Pancreatic Stem Cells
Cardiac Stem Cells
Induced Pluripotent Stem Cells

Millipore 的Millicell系列，提供型号zui为广泛的插入式细胞培养小室：
既有即用式、单个独立无菌包装，也有预装载在培养板上的包装；既有悬挂式，也有站立式培养小室。适用于细胞趋化、侵袭实验，跨内皮层细胞爬行实验，血管生成，神经突触生长，细胞共培养，组织块培养，以及药物运转、渗透等研究。
Millicell插入式培养皿适用于 24孔、12孔或6孔细胞培养板。插入式培养皿易于采用扫描电镜和透射电镜观察，同时它们又和细胞和/或荧光染色兼容。
插入式培养皿的形式
Millicell悬挂式插入培养皿
用于共培养和通透性分析
独特的设计使得进入下孔比其他悬挂式培养皿更容易，污染几率更少 
提供5种不同孔径和3种不同直径的产品，包括光学透明的1 µm孔径产品便于更好的使用显微镜观察 

Millicell器官型插入培养皿
用于高细胞存活和移植体3D结构的高级研究
较低的高度，适于放入标准有盖培养皿中
Biopore™ 膜（PTFE）提供了高的细胞存活率（zui长可至40天）和极佳的跨膜氧气运输 
膜是光学透明的，同时对长期器官型移植物培养进行了优化

Millicell站立式插入培养皿
有助于更好的细胞生长及为细胞研究提供额外的机会
提供Biopore（PTFE）膜，MF膜（混合纤维素）和聚碳酸酯膜

BD Falcon流式管/流式细胞管,圆底试管5ml

BD流式细胞管，流式管

流式细胞仪的流式管，或称流式细胞管,流式细胞用管.

产品品牌: BD Falcon?

产品盖子: 双位置锁扣帽

产品材质: 聚苯乙烯管体, 聚乙烯管盖

RCF级：1400RCF

产品规格: 125/包,8包/箱

BD Falcon流式管/流式细胞管,圆底试管5ml

产品特点：

1、耐用的双位置易于使用，厚壁和独特结构可提供良好密封性

2、可用于特殊流式细胞仪
3、提供医学型包装材料进行的产品独立包装确保无菌

订购信息：

D流式管/试管管