CHIRALPAK IF Semipreparative Column (20mm×250mm×5μm) 品牌:Daicel


CHIRALPAK IF Semipreparative Column (20mm×250mm×5μm)

品牌:Daicel
CAS No.:
储存条件:室温
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

304-98521

1 column 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

Buccutite PE-Cy5抗体标记试剂盒 标记100ug抗体 货号1322-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Buccutite PE-Cy5抗体标记试剂盒 标记100ug抗体

Buccutite PE-Cy5抗体标记试剂盒 标记100ug抗体

Buccutite PE-Cy5抗体标记试剂盒 标记100ug抗体    货号1322 货号 1322 存储条件 在2-8度冷藏保存, 避免光照
规格 2 Labelings 价格 6432
Ex (nm) 565 Em (nm) 666
分子量 溶剂
产品详细介绍

简要概述

Buccutite PE-Cy5抗体标记试剂盒提供了一种以微观方式标记抗体的最便捷方法。PE-Cy5是流式细胞术中流行的一种颜色。其主要吸收峰位于565 nm,发射峰位于674 nm。对于这种串联颜色,推荐使用682/33 nm和695/40 nm的滤光片组。 AAT Bioquest提供这种Buccutite 快速标记试剂盒,以促进PE-Cy5串联结合抗体和其他蛋白质,如链霉抗生物素蛋白和其他二级试剂。 Buccutite PE-Cy5缀合试剂盒提供了一种稳定且便捷的方法来将您的抗体与PE结合。该试剂盒包括活化的PE和反应缓冲液。缀合的抗体可用于WB,ELISA和IHC应用。该试剂盒足以进行2次标记反应,每次反应最多100μg抗体。考虑到PE的大尺寸(240kDa),标记反应中使用的抗体量必须始终小于RPE的量。任何新抗体试剂的最佳比例必须通过实验确定,但每100ug RPE 50-60ug IgG抗体通常可获得最佳结果。我们的试剂盒提供预活化的PE-Cy5,以促进PE-Cy5串联结合抗体和其他蛋白质,如链霉抗生物素蛋白和其他二级试剂。我们预活化的PE-Cy5串联准备结合,比常规繁琐的基于SMCC的结合化学提供更高的产量。此外,我们的预活化PE-Cy5串联通过其蛋白质丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原而再生的硫醇基团。 Buccutite PE-Cy5抗体标记试剂盒是美国AAT Bioquest研发的产品。

点击查看光谱

产品说明书

实验方案

操作步骤

1.运行Antibody-Buccutite MTA反应

1.1将抗体溶液直接加入到Buccutite MTA(组分B)的小瓶中,并通过反复移液几次将其充分混合或将小瓶涡旋几秒钟。

1.2将抗体-Buccutite MTA反应混合物在室温下保持30-60分钟。 注意:如果需要,抗体-Buccutite MTA反应混合物可以旋转或摇动更长时间。

 

2.制备抗体-PE-Cy5缀合物

2.1通过向Buccutite FOL活化的PE-Cy5(组分A)的小瓶中加入50μLddH2O制备Buccutite FOL活化的PE-Cy5溶液,通过反复移液几次充分混合或将小瓶涡旋几秒钟。

2.2将整瓶Buccutite FOL活化的PE-Cy5溶液混合到纯化的抗体-Buccutite MTA溶液(来自步骤纯化抗体-Buccutite MTA溶液)中,充分混合并在室温下旋转混合物1小时。

2.3抗体-PE-Cy5结合物现在可以使用了。 注意:立即使用时,抗体-PE-Cy5结合物需要用您选择的缓冲液稀释。 注意:对于长期储存,需要浓缩或冷冻干燥抗体-PE-Cy5结合物溶液。

 

3.抗体-PE-Cy5共轭物的储存

抗体缀合物应在载体抗体(例如0.1%牛血清白蛋白)存在下以> 0.5mg / mL储存。 当在2mM叠氮化钠存在下储存并避光时,抗体-PE-Cy5缀合物溶液可以在4℃下储存两个月而没有显着变化。 对于更长时间的储存,可以将抗体-PE-Cy5缀合物冻干并在≤-20℃下储存。

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

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Buccutite PE-Cy5抗体标记试剂盒 标记25ug抗体 Cat#1340
Buccutite APC-Cy7抗体标记试剂盒 标记100ug抗体 Cat#1321
Buccutite APC-Cy7抗体标记试剂盒 标记25ug抗体 Cat#1351

说明书
Buccutite PE-Cy5抗体标记试剂盒 标记100ug抗体.pdf

PI3 激酶 p110γ (D55D5) 兔单克隆抗体 PI3 Kinase p110γ (D55D5) Rabbit mAb

PI3 激酶 p110γ (D55D5) 兔单克隆抗体

PI3 Kinase p110γ (D55D5) Rabbit mAb

详细描述:
PI3 Kinase p110γ (D55D5) Rabbit mAb兔单抗可以检测出内源性总PI3K p110γ蛋白。单克隆抗体是采用人类PI3K p110γ蛋白周围残基相对应的合成肽段免疫动物生产的。PI3 Kinase p110-gamma (D55D5) Rabbit mAb detects endogenous levels of total PI3K p110-gamma protein.Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human PI3K p110-gamma.Species predicted to react based on 100% sequence homology: Rat。

应用范围:W IP; 反应种属:Human,Mouse; 灵敏度:Endogenous; MW (kDa):110; Isotype:Rabbit IgG; 标记:无标记。

货号 产品名称 品牌 购买
货号 名称 单位 购买
5405T PI3 激酶 p110γ (D55D5) 兔单克隆抗体 20 µl 商城订购
5405S PI3 激酶 p110γ (D55D5) 兔单克隆抗体 100µl 商城订购

Magazyme试剂盒beta-D-Xylosidase Selenomonas ruminantium E-BXSR

Mezazyme酶试剂盒中国代理商 欢迎访问Megazyme试剂盒官网了解更多信息
β-D-Xylosidase (Selenomonas ruminantium) beta-D-Xylosidase Selenomonas ruminantium E-BXSR beta-D-Xylosidase Selenomonas ruminantium E-BXSR

Product code: E-BXSR-1KU

Content:

€98.00

1,000 Units

Prices exclude VAT

  • Data Sheet
  • SDS
  • COAs
  • Content: 1,000 Units or 3,000 Units
    Shipping Temperature: Ambient
    Storage Temperature: 2-8oC
    Formulation: In 3.2 M ammonium sulphate
    Physical Form: Suspension
    Stability: Minimum 1 year at 4oC. Check vial for details.
    Enzyme Activity: β-Xylosidase
    EC Number: 3.2.1.37
    CAZy Family: GH43
    CAS Number: 9025-53-0
    Synonyms: xylan 1,4-beta-xylosidase; 4-beta-D-xylan xylohydrolase
    Source: Selenomonas ruminantium
    Molecular Weight: 61,900
    Concentration: E-BXSR-1KU: Supplied at ~ 500 U/mL.
    E-BXSR-3KU: Supplied at ~ 500 U/mL.
    Expression: Recombinant from Selenomonas ruminantium
    Specificity: Hydrolysis of (1,4)-β-D-xylans and xylo-oligosaccharides to remove successive D-xylose residues from non-reducing termini.
    Specific Activity: ~ 115 U/mg (40oC, pH 5.3 on pNP-β-D-xylanopyranoside)
    Unit Definition: One Unit of β-xylosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-xylopyranoside (5 mM) in sodium succinate buffer (50 mM), pH 5.3 at 40oC.
    Temperature Optima: 50oC
    pH Optima: 5
    Application examples: Applications in carbohydrate and biofuels research.

    High purity recombinant exo-1,4-β-D-Xylosidase (Selenomonas ruminantium) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

    Data booklets for each pack size are located in the Documents tab.

    We offer other purified CAZy enzymes for various applications.

    Related products
    Similar Products
    E-BXSEBP – β-Xylosidase (Bacillus pumilus)

    Related Carbohydrates
    O-XBI – Xylobiose O-XTR – Xylotriose O-XTE – Xylotetraose O-XPE – Xylopentaose O-XHE – Xylohexaose

    Colourimetric Substrates
    O-PNPX – 4-Nitrophenyl-β-D-xylopyranoside O-PNPX2-10MG – 4-Nitrophenyl-β-xylobioside O-PNPX3 – 4-Nitrophenyl-β-xylotrioside O-4MUX2-5MG – 4-Methylumbelliferyl-β-xylobioside O-ONPX2-5MG – 2-Nitrophenyl-β-xylobioside

    Associated Enzymes
    E-XYTR1 – endo-1,4-β-Xylanase M1 (Trichoderma viride) E-XYTR3 – endo-1,4-β-Xylanase M3
    (Trichoderma longibrachiatum) E-XYAN4 – endo-1,4-β-Xylanase M4 (Aspergillus niger) E-XYRU6 – endo-1,4-β-Xylanase (rumen microorganism) E-XYNBS – endo-1,4-β-Xylanase
    (Bacillus stearothermophilus T6) E-XYNACJ – endo-1,4-β-Xylanase (Cellvibrio japonicus) E-XYLNP – endo-1,4-β-Xylanase (Neocallimastix patriciarum) E-XYLATM – endo-1,4-β-Xylanase (Thermotoga maritima)

    Similar Products
    E-BXSEBP – β-Xylosidase (Bacillus pumilus)
    Related Carbohydrates
    O-XBI – Xylobiose O-XTR – Xylotriose O-XTE – Xylotetraose O-XPE – Xylopentaose O-XHE – Xylohexaose
    Colourimetric Substrates
    O-PNPX – 4-Nitrophenyl-β-D-xylopyranoside O-PNPX2-10MG – 4-Nitrophenyl-β-xylobioside O-PNPX3 – 4-Nitrophenyl-β-xylotrioside O-4MUX2-5MG – 4-Methylumbelliferyl-β-xylobioside O-ONPX2-5MG – 2-Nitrophenyl-β-xylobioside
    Associated Enzymes
    E-XYTR1 – endo-1,4-β-Xylanase M1 (Trichoderma viride) E-XYTR3 – endo-1,4-β-Xylanase M3
    (Trichoderma longibrachiatum) E-XYAN4 – endo-1,4-β-Xylanase M4 (Aspergillus niger) E-XYRU6 – endo-1,4-β-Xylanase (rumen microorganism) E-XYNBS – endo-1,4-β-Xylanase
    (Bacillus stearothermophilus T6) E-XYNACJ – endo-1,4-β-Xylanase (Cellvibrio japonicus) E-XYLNP – endo-1,4-β-Xylanase (Neocallimastix patriciarum) E-XYLATM – endo-1,4-β-Xylanase (Thermotoga maritima)

    beta-D-Xylosidase Selenomonas ruminantium E-BXSR

    Diluted McIlvaine Buffer Solution, pH 6.8 稀McIlvaine缓冲溶液, pH 6.8 品牌:Wako


    品牌:Wako
    CAS No.:
    储存条件:25℃以下
    纯度:
    产品编号

    (生产商编号)

    等级 规格 运输包装 零售价(RMB) 库存情况 参考值

    049-33527

    for Disintegration and Dissolution Test 10 L 咨询


    * 干冰运输、大包装及大批量的产品需酌情添加运输费用


    * 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

    产品描述相关资料下载相关产品浏览记录

    此缓冲溶液可用于医药的崩解和溶出度检测实验中。此试剂通过严格的控制,可以直接用作溶出介质,无需再配置缓冲溶液。
     此产品是根据日本厚生劳动省颁布的以下文件制备的
    1, 仿制药生物等效性实验指导原则,
    2, 处方药质量重新评价的初步实验。

    特点:

    1)采用低溶出类型的容器;

    2)使用的原材料经严格检验,附带证书;

    3)pH在规定值的±0.05以内(25℃);

    4)在标签上清晰表明使用期限。

    Buccutite PerCP抗体标记试剂盒 标记100ug抗体 货号1325-AAT Bioquest荧光染料

    上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

    Buccutite PerCP抗体标记试剂盒 标记100ug抗体

    Buccutite PerCP抗体标记试剂盒 标记100ug抗体

    Buccutite PerCP抗体标记试剂盒 标记100ug抗体    货号1325 货号 1325 存储条件 在2-8度冷藏保存, 避免光照
    规格 2 Labelings 价格 6432
    Ex (nm) 477 Em (nm) 678
    分子量 溶剂
    产品详细介绍

    简要概述

    Buccutite PerCP抗体标记试剂盒提供了一种以微观方式标记抗体的最便捷方法。PerCP(Peridinin-叶绿素 – 蛋白质复合物)从Dinophyceae sp中分离。它具有极高的消光系数,高量子效率和大的斯托克斯位移。氩激光器在488 nm处激发,其最大发射峰在677 nm处。 AAT Bioquest提供这种Buccutite 快速标记试剂盒,以促进PerCP与抗体和其他蛋白质如链霉抗生物素蛋白和其他二级试剂的结合。 Buccutite PerCP共轭试剂盒提供了一种强大而方便的方法,可将抗体与PerCP结合。该试剂盒包括预活化的PerCP和反应缓冲液。缀合的抗体可用于WB,ELISA和IHC应用。该试剂盒足以进行2次标记反应,每次反应最多100μg抗体。任何新抗体试剂的最佳比例必须通过实验确定。我们的试剂盒提供预活化的PerCP,以促进PerCP与抗体和其他蛋白质如链霉抗生物素蛋白和其他二级试剂的结合。我们的预活化PerCP已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。此外,我们的预活化PerCP通过其蛋白质丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原而再生的硫醇基团。Buccutite PerCP抗体标记试剂盒是美国AAT Bioquest研发的产品。

    点击查看光谱

    产品说明书

    实验方案

    操作步骤

    1.运行Antibody-Buccutite MTA反应

    1.1将抗体溶液直接加入到Buccutite MTA(组分B)的小瓶中,并通过反复移液几次将其充分混合或将小瓶涡旋几秒钟。

    1.2将抗体-Buccutite MTA反应混合物在室温下保持30-60分钟。 注意:如果需要,抗体-Buccutite MTA反应混合物可以旋转或摇动更长时间。

     

    2.制备抗体-PerCP缀合物

    2.1通过向Buccutite FOL活化的PerCP(组分A)的小瓶中加入50μLddH2O制备Buccutite FOL活化的PerCP溶液,通过反复移液几次充分混合或将小瓶涡旋几秒钟。

    2.2将整瓶Buccutite FOL活化的PerCP溶液混合到纯化的抗体-Buccutite MTA溶液(来自步骤纯化抗体-Buccutite MTA溶液)中,充分混合并在室温下旋转混合物1小时。

    2.3抗体-PerCP结合物现在可以使用了。 注意:立即使用时,抗体-PerCP结合物需要用您选择的缓冲液稀释。 注意:对于长期储存,需要浓缩或冷冻干燥抗体-PerCP结合物溶液。

     

    3.抗体-PerCP共轭物的储存

    抗体缀合物应在载体抗体(例如0.1%牛血清白蛋白)存在下以> 0.5mg / mL储存。 当在2mM叠氮化钠存在下储存并避光时,抗体-PerCP缀合物溶液可以在4℃下储存两个月而没有显着变化。 对于更长时间的储存,可以将抗体-PerCP缀合物冻干并在≤-20℃下储存。

     

    参考文献

    Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
    Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
    Journal: J Biol Chem (2006): 8573

    Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
    Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
    Journal: Photochem Photobiol Sci (2005): 1016

    Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
    Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
    Journal: Biophys J (2004): 2598

    Evaluation of Tolypothrix germplasm for phycobiliprotein content
    Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
    Journal: Folia Microbiol (Praha) (2003): 59

    Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
    Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
    Journal: Acta Biol Hung (2003): 113

    Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
    Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
    Journal: Mol Microbiol (2002): 749

    Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
    Authors: Ting CS, Rocap G, King J, Chisholm SW.
    Journal: Microbiology (2001): 3171

    Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
    Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
    Journal: FEBS Lett (2000): 9

    Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
    Authors: Triantafilou K, Triantafilou M, Wilson KM.
    Journal: Cytometry (2000): 226

    [Phycobiliprotein and fluorescence immunological assay]
    Authors: Wu P.
    Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

     

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    TRAP1/HSP75 抗体 TRAP1/HSP75 Antibody

    TRAP1/HSP75 抗体

    TRAP1/HSP75 Antibody

    详细描述:
    TRAP1/HSP75 Antibody recognizes endogenous levels of total TRAP1 protein.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu150 of human TRAP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    应用范围:W; 反应种属:Human; 灵敏度:Endogenous; MW (kDa):75; Isotype:Rabbit; 标记:无标记。

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    Magazyme试剂盒AZCL-Amylose I-AZAMY

    Mezazyme酶试剂盒中国代理商 欢迎访问Megazyme试剂盒官网了解更多信息
    AZCL-Amylose

    Product code: I-AZAMY

    €142.00

    5 g

    Prices exclude VAT

  • Assay Protocol
  • SDS
  • COAs
  • FAQs
  • Content: 5 g
    Shipping Temperature: Ambient
    Storage Temperature: Ambient
    Physical Form: Powder
    Stability: > 7 years under recommended storage conditions
    Substrate For (Enzyme): α-amylase
    Assay Format: Spectrophotometer (Semi-quantitative), Petri-dish (Qualitative)
    Detection Method: Absorbance
    Wavelength (nm): 590

    High purity dyed and crosslinked insoluble AZCL-Amylose for identification of enzyme activities in research, microbiological enzyme assays and in vitro diagnostic analysis.

    Dyed and crosslinked amylose. Substrate for the assay of α-amylase.

    View our complete list of insoluble chromogenic substrates.

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    Associated Enzymes
    E-ANAAM – α-Amylase (Aspergillus oryzae) E-BAASS – α-Amylase (Bacillus amyloliquefaciens) E-BLAAM-40ML – α-Amylase (Bacillus licheniformis) E-MAST – Malt Amylase Standard E-MASTP – Malt Amylase Standard (powder)

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    Associated Enzymes
    E-ANAAM – α-Amylase (Aspergillus oryzae) E-BAASS – α-Amylase (Bacillus amyloliquefaciens) E-BLAAM-40ML – α-Amylase (Bacillus licheniformis) E-MAST – Malt Amylase Standard E-MASTP – Malt Amylase Standard (powder)
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    AZCL-Amylose I-AZAMY

    Buccutite PE-Cy5抗体标记试剂盒 标记25ug抗体 货号1340-AAT Bioquest荧光染料

    上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

    Buccutite PE-Cy5抗体标记试剂盒 标记25ug抗体

    Buccutite PE-Cy5抗体标记试剂盒 标记25ug抗体

    Buccutite PE-Cy5抗体标记试剂盒 标记25ug抗体    货号1340 货号 1340 存储条件 在2-8度冷藏保存, 避免光照
    规格 2 Labelings 价格 3840
    Ex (nm) 565 Em (nm) 666
    分子量 溶剂
    产品详细介绍

    简要概述

    Buccutite PE-Cy5抗体标记试剂盒提供了一种以微观方式标记抗体的最便捷方法。PE-Cy5是流式细胞术中常用的颜色。其主要吸收峰位于565nm,发射峰位于674nm。对于这种串联颜色,建议使用682/33 nm和695/40 nm的滤光片组。 AAT Bioquest提供这种Buccutite 快速标记试剂盒,以促进PE-Cy5串联结合抗体和其他蛋白质,如链霉抗生物素蛋白和其他二级试剂。 Buccutite PE-Cy5共轭试剂盒为您的抗体与PE结合提供了一种强大而方便的方法。该试剂盒包括预活化的PE和反应缓冲液。整个过程只需要两次简单的混合,无需进一步纯化。缀合的抗体可用于WB,ELISA和IHC应用。该试剂盒足以进行2次标记反应,每次反应最多25μg抗体。考虑到PE的大尺寸(240kDa),标记反应中使用的抗体量必须始终小于PE的量。任何新抗体试剂的最佳比例必须通过实验确定,但每50μgPE的25μgIgG抗体通常可获得最佳结果。我们的试剂盒提供预活化的PE-Cy5,以促进PE-Cy5串联结合抗体和其他蛋白质,如链霉抗生物素蛋白和其他二级试剂。我们的预活化PE-Cy5串联已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。此外,我们的预活化PE-Cy5串联通过其蛋白质中丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原而再生的硫醇基团。Buccutite PE-Cy5抗体标记试剂盒是美国AAT Bioquest研发的产品。

    点击查看光谱

    产品说明书

    实验方案

    操作步骤

    1.运行Antibody-Buccutite MTA反应

    1.1将抗体溶液直接加入到Buccutite MTA(组分B)的小瓶中,并通过反复移液几次将其充分混合或将小瓶涡旋几秒钟。

    1.2将抗体-Buccutite MTA反应混合物在室温下保持30-60分钟。 注意:如果需要,抗体-Buccutite MTA反应混合物可以旋转或摇动更长时间。

     

    2.制备抗体-PE-Cy5缀合物

    2.1通过向Buccutite FOL活化的PE-Cy5(组分A)的小瓶中加入50μLddH2O制备Buccutite FOL活化的PE-Cy5溶液,通过反复移液几次充分混合或将小瓶涡旋几秒钟。

    2.2将整瓶Buccutite FOL活化的PE-Cy5溶液混合到纯化的抗体-Buccutite MTA溶液(来自步骤纯化抗体-Buccutite MTA溶液)中,充分混合并在室温下旋转混合物1小时。

    2.3抗体-PE-Cy5结合物现在可以使用了。 注意:立即使用时,抗体-PE-Cy5结合物需要用您选择的缓冲液稀释。 注意:对于长期储存,需要浓缩或冷冻干燥抗体-PE-Cy5结合物溶液。

     

    3.抗体-PE-Cy5共轭物的储存

    抗体缀合物应在载体抗体(例如0.1%牛血清白蛋白)存在下以> 0.5mg / mL储存。 当在2mM叠氮化钠存在下储存并避光时,抗体-PE-Cy5缀合物溶液可以在4℃下储存两个月而没有显着变化。 对于更长时间的储存,可以将抗体-PE-Cy5缀合物冻干并在≤-20℃下储存。

     

    参考文献

    Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
    Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
    Journal: J Biol Chem (2006): 8573

    Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
    Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
    Journal: Photochem Photobiol Sci (2005): 1016

    Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
    Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
    Journal: Biophys J (2004): 2598

    Evaluation of Tolypothrix germplasm for phycobiliprotein content
    Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
    Journal: Folia Microbiol (Praha) (2003): 59

    Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
    Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
    Journal: Acta Biol Hung (2003): 113

    Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
    Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
    Journal: Mol Microbiol (2002): 749

    Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
    Authors: Ting CS, Rocap G, King J, Chisholm SW.
    Journal: Microbiology (2001): 3171

    Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
    Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
    Journal: FEBS Lett (2000): 9

    Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
    Authors: Triantafilou K, Triantafilou M, Wilson KM.
    Journal: Cytometry (2000): 226

    [Phycobiliprotein and fluorescence immunological assay]
    Authors: Wu P.
    Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

     

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    DDB-2 (D4C4) 兔单克隆抗体

    DDB-2 (D4C4) Rabbit mAb

    详细描述:
    DDB2(D4C4)兔单抗识别内源性的DDB2总蛋白。DDB2(D4C4)兔单抗是通过合成与人DDB2蛋白Ala174邻近的氨基酸残基序列一致的肽段,免疫动物获得。Species predicted to react based on 100% sequence homology: Rat, Monkey.

    应用范围:W; 反应种属:Human,Mouse; 灵敏度:Endogenous; MW (kDa):43; Isotype:Rabbit IgG; 标记:无标记。

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    Magazyme试剂盒AZCL-Arabinan Debranched I-AZDAR

    Mezazyme酶试剂盒中国代理商 欢迎访问Megazyme试剂盒官网了解更多信息
    AZCL-Arabinan (Debranched)

    Product code: I-AZDAR

    €168.00

    3 g

    Prices exclude VAT

  • Assay Protocol
  • SDS
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  • Content: 3 g
    Shipping Temperature: Ambient
    Storage Temperature: Below -10oC
    Physical Form: Powder
    Stability: > 9 years under recommended storage conditions
    Substrate For (Enzyme): endo-Arabinanase
    Assay Format: Spectrophotometer (Semi-quantitative), Petri-dish (Qualitative)
    Detection Method: Absorbance
    Wavelength (nm): 590

    High purity dyed and crosslinked insoluble AZCL-Arabinan (Debranched) for identification of enzyme activities in research, microbiological enzyme assays and in vitro diagnostic analysis. 

    Substrate for the assay of endo-1,5-α-L-arabinanase.

    Display our complete list of enzyme substrate products.

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    OGDH 抗体 OGDH Antibody

    OGDH 抗体

    OGDH Antibody

    详细描述:
    OGDH Antibody recognizes endogenous levels of total OGDH protein.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala110 of mouse OGDH protein. Antibodies are purified by protein A and peptide affinity chromatography.

    应用范围:W IP; 反应种属:Human,Mouse,Rat,Monkey; 灵敏度:Endogenous; MW (kDa):110; Isotype:Rabbit; 标记:无标记。

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